Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,303,881	A→G	F77L (TTC→CTC)	mdtN ←	putative multidrug efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,303,881	0	A	G	100.0%	258.0 / NA	72	F77L (TTC→CTC)	mdtN	putative multidrug efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (38/34); total (38/34)

GCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGATGGCGCACTGTCTACGCG  >  NC_000913/4303748‑4304016

gCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCaa                                                                                                                                   >  1:1282783/1‑140 (MQ=255)

gCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCaa                                                                                                                                   >  2:151925/1‑140 (MQ=255)

    tAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCg                                                                                                                               <  1:1320383/140‑1 (MQ=255)

    tAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCg                                                                                                                               <  2:521101/140‑1 (MQ=255)

      aCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGcc                                                                                                                             <  1:1312559/140‑1 (MQ=255)

      aCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGcc                                                                                                                             >  1:27972/1‑140 (MQ=255)

          aGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGt                                                                                                                         >  1:90095/1‑140 (MQ=255)

           gTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGtt                                                                                                                        >  1:62324/1‑140 (MQ=255)

            tCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGttt                                                                                                                       >  2:940863/1‑140 (MQ=255)

            tCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGttt                                                                                                                       >  1:1348483/1‑140 (MQ=255)

            tCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCCCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGttt                                                                                                                       >  2:858660/1‑140 (MQ=255)

              ggCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGa                                                                                                                     <  2:90095/140‑1 (MQ=255)

                cACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGCACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACt                                                                                                                   >  2:1104775/1‑140 (MQ=255)

                    aaaCTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCt                                                                                                               >  2:992842/1‑140 (MQ=255)

                          ttGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGt                                                                                                         >  2:452733/1‑140 (MQ=255)

                               cgTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTg                                                                                                    >  2:654503/1‑140 (MQ=255)

                               cgTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTg                                                                                                    >  2:90485/1‑140 (MQ=255)

                                 tCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGAc                                                                                                  >  1:824794/1‑140 (MQ=255)

                                  cAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGAcc                                                                                                 <  1:1400257/140‑1 (MQ=255)

                                  cAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGAcc                                                                                                 >  1:359575/1‑140 (MQ=255)

                                  cAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGAcc                                                                                                 <  2:1216177/140‑1 (MQ=255)

                                  cAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGAcc                                                                                                 >  1:1216177/1‑140 (MQ=255)

                                    acgctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGAcc                                                                                                 <  2:801643/138‑1 (MQ=255)

                                    acgctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGAcc                                                                                                 >  1:801643/1‑138 (MQ=255)

                                    acgctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGc                                                                                               <  2:429791/140‑1 (MQ=255)

                                    acgctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGc                                                                                               <  2:307806/140‑1 (MQ=255)

                                    acgctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGc                                                                                               <  2:27972/140‑1 (MQ=255)

                                    acgctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGc                                                                                               <  1:992842/140‑1 (MQ=255)

                                            ctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTa                                                                                       >  2:1182963/1‑140 (MQ=255)

                                            ctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTa                                                                                       >  2:711837/1‑140 (MQ=255)

                                               ggTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACaa                                                                                    >  2:202740/1‑140 (MQ=255)

                                                 tGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATg                                                                                  >  2:1074137/1‑140 (MQ=255)

                                                    gCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCgg                                                                               >  1:899971/1‑140 (MQ=255)

                                                    gCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCgg                                                                               >  1:1048567/1‑140 (MQ=255)

                                                         aTTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCt                                                                          >  2:694829/1‑140 (MQ=255)

                                                         aTTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCt                                                                          >  1:22943/1‑140 (MQ=255)

                                                          tttGCTTATGCAGCGCCGCGAGGGAGCCTTAAGCTTTCGCGAGATTGGTTTCGTACGGGCGCGGGTCGATGCGGGGCAGAAAATCGCCCTGTTTGCCTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTg                                                                         <  2:62324/140‑1 (MQ=255)

                                                          tttGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTg                                                                         <  2:824794/140‑1 (MQ=255)

                                                           ttGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGa                                                                        <  1:694829/140‑1 (MQ=255)

                                                               ttATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTc                                                                    >  2:337117/1‑140 (MQ=255)

                                                               ttATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTCCCGGGTTGCCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTc                                                                    <  2:1364553/140‑1 (MQ=255)

                                                                  tCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCTATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCgg                                                                 >  2:58628/1‑140 (MQ=255)

                                                                  tCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCgg                                                                 >  1:326102/1‑140 (MQ=255)

                                                                     ggcgcCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGGGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTAc                                                              <  2:667485/139‑1 (MQ=255)

                                                                     aGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTAc                                                              <  1:1210788/140‑1 (MQ=255)

                                                                     aGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTAc                                                              <  1:1074137/140‑1 (MQ=255)

                                                                       cgccgcGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTAc                                                              >  1:213643/1‑138 (MQ=255)

                                                                       cgccgcGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTAc                                                              <  2:213643/138‑1 (MQ=255)

                                                                       cgccgcGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACca                                                            >  2:58891/1‑140 (MQ=255)

                                                                       cgccgcGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACca                                                            <  1:831828/140‑1 (MQ=255)

                                                                         ccgcGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACcaca                                                          <  2:1296939/140‑1 (MQ=255)

                                                                            cgAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCTATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCa                                                       <  1:58628/140‑1 (MQ=255)

                                                                            cgAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCa                                                       <  1:151925/140‑1 (MQ=255)

                                                                            cgAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCa                                                       >  1:225921/1‑140 (MQ=255)

                                                                            cgAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCa                                                       >  1:984033/1‑140 (MQ=255)

                                                                            cgAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCa                                                       >  2:849448/1‑140 (MQ=255)

                                                                             gagggaggCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCaa                                                      <  1:202740/140‑1 (MQ=255)

                                                                               gggaggCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATg                                                    <  1:621286/140‑1 (MQ=255)

                                                                                                gCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTa                                   <  1:90485/140‑1 (MQ=255)

                                                                                                 ccAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTaa                                  <  1:243361/140‑1 (MQ=255)

                                                                                                   aGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGc                                <  2:1048567/140‑1 (MQ=255)

                                                                                                   aGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGc                                <  1:858660/140‑1 (MQ=255)

                                                                                                    gtttGGCTTCGTACGGGGGCGGGTCGATGCGGAGCAGCAACGCGCCCTGTTTGATTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGATGACTTCCGGTGCCACATCGATGGTATCTGCTGGCGCGGAAGCg                               <  2:265150/138‑1 (MQ=255)

                                                                                                     aTTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAATTCGCCCTGTTTGACTGCCTGGTTGGCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGt                              <  2:1282783/140‑1 (MQ=255)

                                                                                                     aTTGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGt                              <  1:1182963/140‑1 (MQ=255)

                                                                                                       tGGCTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCa                            <  1:849448/140‑1 (MQ=255)

                                                                                                          cTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTa                         >  1:761208/1‑140 (MQ=255)

                                                                                                          cTTCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTa                         >  1:61172/1‑140 (MQ=255)

                                                                                                            tCGTACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGt                       <  1:940863/140‑1 (MQ=255)

                                                                                                               tACGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGa                    >  1:1414440/1‑140 (MQ=255)

                                                                                                                aCGGGCGCGGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGAt                   <  1:452733/140‑1 (MQ=255)

                                                                                                                      gcgGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGATGGCGCa             <  2:326102/140‑1 (MQ=255)

                                                                                                                       cgGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTCCAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGATGGCGCAc            <  2:761208/140‑1 (MQ=255)

                                                                                                                       cgGGTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGATGGCGCAc            >  2:1139617/1‑140 (MQ=255)

                                                                                                                        gggTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGATGGCGCACt           >  2:1359283/1‑140 (MQ=255)

                                                                                                                        gggTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGATGGCGCACt           <  2:1348483/140‑1 (MQ=255)

                                                                                                                         ggTCGATGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGATGGCGCACTg          >  1:1086865/1‑140 (MQ=255)

                                                                                                                               tGCGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGATGGCGCACTGTCTAcg    <  2:61172/140‑1 (MQ=255)

                                                                                                                                 cGGAGCAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGATGGCGCACTGTCTAcgcg  <  1:1277124/140‑1 (MQ=255)

GCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTTGATGGCGCACTGTCTACGCG  >  NC_000913/4303748‑4304016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A