Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,330,134 A→G T259A (ACC→GCC)  yjcZ → uncharacterized protein YjcZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,330,1340AG95.7% 303.4 / 2.3 92T259A (ACC→GCC) yjcZuncharacterized protein YjcZ
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (53/35);  total (55/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

CGGCACCGACCACGCGTCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGACCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAATGAGTCCTAAACGAA  >  NC_000913/4329997‑4330270
                                                                                                                                         |                                                                                                                                        
cGGCACCGACCACGCGTCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGGGCATGCTGATTTTTTAAGGTGGCTGGGAAAGGGGACCACGGCAGATAAACAACCCGTTTTCTGGTCAGTGGcc                                                                                                                                        >  2:766039/1‑140 (MQ=255)
cGGCACCGACCACGCGTCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGcc                                                                                                                                        >  2:1195308/1‑140 (MQ=255)
cGGCACCGACCACGCGTCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGcc                                                                                                                                        >  1:1153280/1‑140 (MQ=255)
 ggCACCGACCACGCGTCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGccc                                                                                                                                       <  2:865899/140‑1 (MQ=255)
  gCACCGACCACGCGTCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCa                                                                                                                                      >  2:1009644/1‑140 (MQ=255)
     ccGACCACGCGTCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACg                                                                                                                                   <  2:1153280/140‑1 (MQ=255)
     ccGACCACGCGTCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACg                                                                                                                                   <  1:128716/140‑1 (MQ=255)
      cGACCACGCGTCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTGGCTGGTCAGTGGCCCAACGc                                                                                                                                  <  1:239755/140‑1 (MQ=255)
                tCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTg                                                                                                                        <  1:490405/140‑1 (MQ=255)
                    tATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTcgc                                                                                                                    <  2:604183/140‑1 (MQ=255)
                     aTTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTcgcg                                                                                                                   <  1:613984/140‑1 (MQ=255)
                     aTTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTcgcg                                                                                                                   <  1:663107/140‑1 (MQ=255)
                          cATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTggg                                                                                                              <  1:1195308/140‑1 (MQ=255)
                          cATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTggg                                                                                                              <  2:86778/140‑1 (MQ=255)
                           aTCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTgggg                                                                                                             >  1:1157653/1‑140 (MQ=255)
                            tCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGACCCAACGCTGGCAAACTGTCGCGCTGGGGa                                                                                                            >  1:805608/1‑140 (MQ=255)
                              aGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATg                                                                                                          >  2:152529/1‑140 (MQ=255)
                              aGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATg                                                                                                          >  2:674254/1‑140 (MQ=255)
                              aGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATg                                                                                                          >  1:352124/1‑140 (MQ=255)
                              aGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATg                                                                                                          >  1:1394789/1‑140 (MQ=255)
                              aGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCGGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAATCTGTCGCGCTGGGGATg                                                                                                          >  2:1258516/1‑140 (MQ=255)
                                tGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGcc                                                                                                        >  1:667254/1‑140 (MQ=255)
                                tGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGcc                                                                                                        >  1:659162/1‑140 (MQ=255)
                                tGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGcc                                                                                                        >  2:718477/1‑140 (MQ=255)
                                tGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGcc                                                                                                        >  2:590351/1‑140 (MQ=255)
                                tGGTTTGACTCTTCGACCGTTCCGACGGGCGGGGAGCATGCAGATTTTTTAAGTTGGCTGGGAAAGTCGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCACCGCTGGCAAACTGGCGCGCTGGGGATGcc                                                                                                        >  2:312166/1‑140 (MQ=255)
                                 ggTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCa                                                                                                       <  2:21656/140‑1 (MQ=255)
                                 ggTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCa                                                                                                       <  1:536350/140‑1 (MQ=255)
                                 ggTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCa                                                                                                       <  1:674254/140‑1 (MQ=255)
                                  gTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCAc                                                                                                      >  2:376266/1‑140 (MQ=255)
                                  gTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTATCGCGCTGGGGATGCCAc                                                                                                      >  1:629064/1‑140 (MQ=255)
                                   tttGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACg                                                                                                     >  1:646884/1‑140 (MQ=255)
                                      gCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGAct                                                                                                  <  1:718477/140‑1 (MQ=255)
                                      gCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGAct                                                                                                  <  1:559412/140‑1 (MQ=255)
                                      gCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGAct                                                                                                  >  2:488497/1‑140 (MQ=255)
                                            tCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTc                                                                                            >  1:1179525/1‑140 (MQ=255)
                                               aCCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGAct                                                                                                  <  1:1407764/131‑1 (MQ=255)
                                               aCCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGAct                                                                                                  >  2:1407764/1‑131 (MQ=255)
                                                ccGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTcagcg                                                                                        >  1:1000725/1‑140 (MQ=255)
                                                   ttCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTcagcgcag                                                                                     >  2:111374/1‑140 (MQ=255)
                                                     ccGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTcagcgcagcg                                                                                   >  1:475949/1‑140 (MQ=255)
                                                      cGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGt                                                                                  <  1:1226709/140‑1 (MQ=255)
                                                        aCGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCt                                                                                >  2:831882/1‑140 (MQ=255)
                                                          gggcgggGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTgg                                                                              >  1:1265462/1‑140 (MQ=255)
                                                           ggcgggGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGc                                                                             <  1:488497/140‑1 (MQ=255)
                                                            gcgggGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCg                                                                            <  2:352124/140‑1 (MQ=255)
                                                                ggAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCggggg                                                                        <  2:667254/140‑1 (MQ=255)
                                                                  aGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGACCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCa                                                                      <  2:805608/140‑1 (MQ=255)
                                                                      tGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGc                                                                  >  1:617329/1‑140 (MQ=255)
                                                                      tGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGc                                                                  >  1:1029493/1‑140 (MQ=255)
                                                                      tGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGc                                                                  >  2:181837/1‑140 (MQ=255)
                                                                      tGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGc                                                                  >  2:658605/1‑140 (MQ=255)
                                                                         tGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCg                                                               <  1:376266/140‑1 (MQ=255)
                                                                          gATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGa                                                              >  1:1028624/1‑140 (MQ=255)
                                                                                ttAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAAt                                                        <  2:1029493/140‑1 (MQ=255)
                                                                                   aGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAAtctt                                                     <  1:1009644/140‑1 (MQ=255)
                                                                                   aGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAAtctt                                                     <  1:1351384/140‑1 (MQ=255)
                                                                                       ggCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTctct                                                 >  2:1054079/1‑140 (MQ=255)
                                                                                       ggCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTctct                                                 >  2:42129/1‑140 (MQ=255)
                                                                                       ggCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTctct                                                 >  2:660084/1‑140 (MQ=255)
                                                                                        gCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTg                                                >  1:679495/1‑140 (MQ=255)
                                                                                         cTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGt                                               >  1:72674/1‑140 (MQ=255)
                                                                                                  tGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGc                                      >  2:369515/1‑140 (MQ=255)
                                                                                                  tGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGc                                      >  1:1359911/1‑140 (MQ=255)
                                                                                                  tGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGACCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGc                                      >  1:977243/1‑140 (MQ=255)
                                                                                                   ggACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCGAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCg                                     <  1:572768/140‑1 (MQ=255)
                                                                                                   ggACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCg                                     <  1:111374/140‑1 (MQ=255)
                                                                                                        aCGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAaat                                <  2:646884/140‑1 (MQ=255)
                                                                                                         cGGCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAaata                               >  2:185592/1‑140 (MQ=255)
                                                                                                          ggCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAaataa                              <  1:1054079/140‑1 (MQ=255)
                                                                                                           gCAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAaataat                             >  1:692206/1‑140 (MQ=255)
                                                                                                            cAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATc                            <  2:142504/140‑1 (MQ=255)
                                                                                                            cAGATAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATc                            >  2:92524/1‑140 (MQ=255)
                                                                                                               aTAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGt                         <  2:1359911/140‑1 (MQ=255)
                                                                                                                tAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCAGGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGtt                        >  2:1260849/1‑140 (MQ=255)
                                                                                                                tAAACAACCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGtt                        <  1:590351/140‑1 (MQ=255)
                                                                                                                  aacaacCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTAc                      >  1:129894/1‑140 (MQ=255)
                                                                                                                      acCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAaataa                              >  2:673403/1‑128 (MQ=255)
                                                                                                                      acCCGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAaataa                              <  1:673403/128‑1 (MQ=255)
                                                                                                                        ccGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAg                          >  2:356895/1‑130 (MQ=255)
                                                                                                                        ccGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAg                          <  1:356895/130‑1 (MQ=255)
                                                                                                                        ccGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAAt                >  1:1230228/1‑140 (MQ=255)
                                                                                                                        ccGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAAt                <  1:181837/140‑1 (MQ=255)
                                                                                                                        ccGTTTGCTGGTCAGTGACCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAAt                <  2:977243/140‑1 (MQ=255)
                                                                                                                         cGTTTGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAATg               >  1:880494/1‑140 (MQ=255)
                                                                                                                           tttGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAATGAg             >  1:189110/1‑140 (MQ=255)
                                                                                                                             tGCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAATGAGTc           >  2:1101172/1‑140 (MQ=255)
                                                                                                                              gCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATCATCAGTTACATCAATGAGTcc          <  2:1000725/140‑1 (MQ=255)
                                                                                                                              gCTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAATGAGTcc          >  2:1056554/1‑140 (MQ=255)
                                                                                                                               cTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAATGAGTCCt         >  2:486205/1‑140 (MQ=255)
                                                                                                                               cTGGTCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAATGAGTCCt         >  1:202933/1‑140 (MQ=255)
                                                                                                                                  gTCAGTGGCCCAACGCTGGCAAACTGTCGCGTTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAATGAGTCCTaaa      >  2:216676/1‑140 (MQ=255)
                                                                                                                                   tCAGTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGTGTAAATAATCAGTTACATCAATGAGTCCTAAAc     >  2:128322/1‑140 (MQ=255)
                                                                                                                                      gTGGCCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAATGAGTCCTAAACGaa  >  1:1008858/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CGGCACCGACCACGCGTCACTATTACCATCAGTGGTTTGCCTCTTCGACCGTTCCGACGGGCGGGGAGCATGCTGATTTTTTAAGTTGGCTGGGAAAGTGGACCACGGCAGATAAACAACCCGTTTGCTGGTCAGTGACCCAACGCTGGCAAACTGTCGCGCTGGGGATGCCACGACTCTGTTCAGCGCAGCGTCTGGCGGGGGCAATGCTCGAGGAAATCTTCTCTGTAAATTTGGCGTAAATAATCAGTTACATCAATGAGTCCTAAACGAA  >  NC_000913/4329997‑4330270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A