Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,591	A→G	noncoding (9/76 nt)	glyY →	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,591	0	A	G	98.4%	216.6 / NA	63	noncoding (9/76 nt)	glyY	tRNA‑Gly
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (35/27); minor base T (1/0); total (36/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACT  >  NC_000913/4392454‑4392730

aaaGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGc                                                                                                                                           <  1:381273/140‑1 (MQ=255)

    cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAg                                                                                                                                       >  1:1053904/1‑140 (MQ=255)

    cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAg                                                                                                                                       >  2:1079422/1‑140 (MQ=255)

    cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAg                                                                                                                                       >  1:197102/1‑140 (MQ=255)

     acaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGt                                                                                                                                      <  1:790335/140‑1 (MQ=255)

        gACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTgg                                                                                                                                   <  2:1389874/140‑1 (MQ=255)

                   cGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGAc                                                                                                                        <  2:919331/140‑1 (MQ=255)

                   cGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGAc                                                                                                                        <  2:667898/140‑1 (MQ=255)

                     ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCAGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCt                                                                                                                      >  2:450612/1‑140 (MQ=255)

                            cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                                               >  1:7613/1‑140 (MQ=255)

                            cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTACTGTAAGGCACAGACCACCCAAGCGGGAAATGCTCAGTTGGTAGAGCACGAGCTTGCCAAg                                                                                                               >  2:480365/1‑140 (MQ=255)

                                gTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                                           >  1:224852/1‑140 (MQ=255)

                                 ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                                          >  2:244301/1‑140 (MQ=255)

                                      agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcg                                                                                                     >  1:88959/1‑140 (MQ=255)

                                         gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTGGAGCACGAGCTTGCCAAGGTCGGGGTCGCGa                                                                                                  >  2:986840/1‑140 (MQ=255)

                                         gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                                                                                                  >  2:1053491/1‑140 (MQ=255)

                                         gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                                                                                                  >  2:513472/1‑140 (MQ=255)

                                          cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGcc                                                                                                                  <  2:858706/123‑1 (MQ=255)

                                          cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGcc                                                                                                                  >  1:858706/1‑123 (MQ=255)

                                          cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAg                                                                                                 >  1:1400174/1‑140 (MQ=255)

                                          cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAg                                                                                                 >  2:152201/1‑140 (MQ=255)

                                          cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAg                                                                                                 >  2:277091/1‑140 (MQ=255)

                                           aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGt                                                                                                >  1:1071949/1‑140 (MQ=255)

                                               ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                            <  2:1400174/140‑1 (MQ=255)

                                                  tGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtc                                                                                         >  2:938735/1‑140 (MQ=255)

                                                   gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                                        >  1:934785/1‑140 (MQ=255)

                                                   gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                                        <  1:269667/140‑1 (MQ=255)

                                                   gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                                        >  2:845486/1‑140 (MQ=255)

                                                         gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                                  >  1:780020/1‑140 (MQ=255)

                                                         gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                                  >  1:525936/1‑140 (MQ=255)

                                                         gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                                  >  1:1337801/1‑140 (MQ=255)

                                                             ggggCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGGTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGc                                                                              >  2:269667/1‑140 (MQ=255)

                                                                         cGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAttcttc                                                                  >  1:900039/1‑140 (MQ=255)

                                                                         cGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAttcttc                                                                  <  2:1071949/140‑1 (MQ=255)

                                                                          gAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATtcttct                                                                 <  1:7016/140‑1 (MQ=255)

                                                                          gAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATtcttct                                                                 <  1:1079422/140‑1 (MQ=255)

                                                                          gAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATtcttct                                                                 <  2:197102/140‑1 (MQ=255)

                                                                            gTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctct                                                               <  2:1399088/140‑1 (MQ=255)

                                                                                   ttCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATaaa                                                        <  2:900039/140‑1 (MQ=255)

                                                                                              aaaTTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGc                                             <  1:766746/140‑1 (MQ=255)

                                                                                               aaTTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCg                                            >  1:106094/1‑140 (MQ=255)

                                                                                                  ttGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcg                                         <  2:1337801/140‑1 (MQ=255)

                                                                                                  ttGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcg                                         <  1:152201/140‑1 (MQ=255)

                                                                                                  ttGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcg                                         <  2:7613/140‑1 (MQ=255)

                                                                                                  ttGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcg                                         <  2:525936/140‑1 (MQ=255)

                                                                                                    gAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcgcg                                       >  1:263968/1‑140 (MQ=255)

                                                                                                    gAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcgcg                                       <  1:244301/140‑1 (MQ=255)

                                                                                                     aaaGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGa                                      <  1:1063741/140‑1 (MQ=255)

                                                                                                     aaaGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGa                                      <  1:277091/140‑1 (MQ=255)

                                                                                                     aaaGTGCTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGa                                      <  2:1343362/140‑1 (MQ=255)

                                                                                                           cTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGtt                                >  2:1110867/1‑140 (MQ=255)

                                                                                                           cTGTAAGGCACAGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGtt                                >  1:951599/1‑140 (MQ=255)

                                                                                                                   cacaGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTGAATAAAATATCTACAGCGACGCGATGCGTTATTGCTgg                        >  2:1184184/1‑140 (MQ=255)

                                                                                                                   cacaGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTgg                        >  2:829574/1‑140 (MQ=255)

                                                                                                                     caGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGtt                      >  2:541133/1‑140 (MQ=255)

                                                                                                                      aGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGttt                     >  2:897493/1‑140 (MQ=255)

                                                                                                                      aGACCACCCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGttt                     >  1:1179483/1‑140 (MQ=255)

                                                                                                                         ccaccCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttg                  <  1:480365/140‑1 (MQ=255)

                                                                                                                          caccCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgt                 <  1:450612/140‑1 (MQ=255)

                                                                                                                          caccCAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgt                 >  1:217958/1‑140 (MQ=255)

                                                                                                                            cccAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgttg               >  1:124279/1‑140 (MQ=255)

                                                                                                                            cccAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgttg               >  2:1117943/1‑140 (MQ=255)

                                                                                                                            cccAAGCGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGCTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgttg               >  2:998817/1‑140 (MQ=255)

                                                                                                                                  cGGGAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTg         >  1:1135256/1‑140 (MQ=255)

                                                                                                                                     gAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACa      <  2:780020/140‑1 (MQ=255)

                                                                                                                                     gAATGGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACa      <  1:845486/140‑1 (MQ=255)

                                                                                                                                         aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAAct  <  2:534554/140‑1 (MQ=255)

AAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACT  >  NC_000913/4392454‑4392730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A