Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,436,066	C→T	G167E (GGA→GAA)	cpdB ←	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,436,066	0	C	T	96.7%	417.6 / 4.7	120	coding (500/1944 nt)	cpdB	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (53/63); minor base . (2/2); total (55/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 120 total aligned reads displayed.

CATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGAC  >  NC_000913/4435930‑4436201

cATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGt                                                                                                                                      <  2:512303/140‑1 (MQ=255)

   ttCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCtt                                                                                                                                   >  1:19070/1‑140 (MQ=255)

         cACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGa                                                                                                                             <  2:89070/140‑1 (MQ=255)

         cACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGa                                                                                                                             <  1:567892/140‑1 (MQ=255)

         cACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGa                                                                                                                             <  1:1323119/140‑1 (MQ=255)

          aCGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGAc                                                                                                                            <  2:649177/140‑1 (MQ=255)

           cGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGAcc                                                                                                                           <  2:584682/140‑1 (MQ=255)

             tATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCAc                                                                                                                         >  2:156804/1‑140 (MQ=255)

             tATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCAc                                                                                                                         >  2:503468/1‑140 (MQ=255)

              aTTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACt                                                                                                                        <  1:351228/140‑1 (MQ=255)

                 tGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCg                                                                                                                     <  1:1034579/140‑1 (MQ=255)

                 tGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCg                                                                                                                     <  2:1116402/140‑1 (MQ=255)

                   cgcACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGgt                                                                                                                   <  2:254927/140‑1 (MQ=255)

                   cgcACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGgt                                                                                                                   <  1:563174/140‑1 (MQ=255)

                   cgcACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGgt                                                                                                                   <  2:871455/140‑1 (MQ=255)

                     cACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGgtgt                                                                                                                 >  1:1289960/1‑140 (MQ=255)

                      aCGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTc                                                                                                                >  1:819727/1‑140 (MQ=255)

                      aCGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTc                                                                                                                <  1:1193458/140‑1 (MQ=255)

                        ggTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGAc                                                                                                                            <  1:1406367/126‑1 (MQ=255)

                        ggTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGAc                                                                                                                            >  2:1406367/1‑126 (MQ=255)

                        ggTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCtt                                                                                                              >  1:1380033/1‑140 (MQ=255)

                        ggTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCtt                                                                                                              >  1:628378/1‑140 (MQ=255)

                        ggTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCtt                                                                                                              <  2:834556/140‑1 (MQ=255)

                        ggTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCtt                                                                                                              >  1:120295/1‑140 (MQ=255)

                          tttCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTtta                                                                                                            <  2:883075/140‑1 (MQ=255)

                                 aaTATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAAt                                                                                                     <  2:120295/140‑1 (MQ=255)

                                  atatCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATa                                                                                                    <  1:1002082/140‑1 (MQ=255)

                                  atatCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATa                                                                                                    <  2:344070/140‑1 (MQ=255)

                                  atatCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATa                                                                                                    <  1:156804/140‑1 (MQ=255)

                                  atatCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGATGTCTTTAATTAAATa                                                                                                    <  1:437112/140‑1 (MQ=255)

                                   tatCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATAc                                                                                                   >  1:709971/1‑140 (MQ=255)

                                    atCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCtt                                                                                                              <  1:217914/128‑1 (MQ=255)

                                    atCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCtt                                                                                                              >  2:217914/1‑128 (MQ=255)

                                    atCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACg                                                                                                  >  2:509833/1‑140 (MQ=255)

                                    atCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACg                                                                                                  >  2:305749/1‑140 (MQ=255)

                                    atCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACg                                                                                                  >  2:804955/1‑140 (MQ=255)

                                        ttCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATa                                                                                                    >  2:81900/1‑134 (MQ=255)

                                        ttCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGAAGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGgtgt                                                                                              >  2:1262850/1‑140 (MQ=255)

                                         tCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTa                                                                                             <  1:1250409/140‑1 (MQ=255)

                                               tCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCATGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACAtt                                                                                       >  2:914593/1‑140 (MQ=255)

                                                 aCTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTgg                                                                                     <  1:424462/140‑1 (MQ=255)

                                                 aCTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTgg                                                                                     <  1:1262850/140‑1 (MQ=255)

                                                      cccGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGtt                                                                                <  1:509833/140‑1 (MQ=255)

                                                      cccGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGtt                                                                                <  2:709971/140‑1 (MQ=255)

                                                               aTTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCGCTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTgg                                                                       >  1:720052/1‑140 (MQ=255)

                                                               aTTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTgg                                                                       >  1:993056/1‑140 (MQ=255)

                                                               aTTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTgg                                                                       <  2:123323/140‑1 (MQ=255)

                                                                    cTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCa                                                                  >  2:359799/1‑140 (MQ=255)

                                                                    cTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCa                                                                  <  2:628378/140‑1 (MQ=255)

                                                                    cTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGACTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCa                                                                  >  2:983252/1‑140 (MQ=255)

                                                                      ttATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAAt                                                                <  2:713658/140‑1 (MQ=255)

                                                                         tCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGAc                                                             >  2:644908/1‑140 (MQ=255)

                                                                          cccAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACg                                                            <  2:813063/140‑1 (MQ=255)

                                                                           ccAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGt                                                           <  1:804955/140‑1 (MQ=255)

                                                                            cAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGtt                                                          <  2:966018/140‑1 (MQ=255)

                                                                            cAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGtt                                                          <  2:566309/140‑1 (MQ=255)

                                                                              gCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCaa                                                                 <  1:136102/131‑1 (MQ=255)

                                                                              gCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCaa                                                                 >  2:136102/1‑131 (MQ=255)

                                                                              gCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTgg                                                        >  1:894452/1‑140 (MQ=255)

                                                                                 cATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACg                                                            >  2:1251397/1‑133 (MQ=255)

                                                                                 cATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACg                                                            <  1:1251397/133‑1 (MQ=255)

                                                                                  aTGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGGCCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCAtt                                                    <  2:220742/140‑1 (MQ=255)

                                                                                   tGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAAt                                                                >  2:1270960/1‑127 (MQ=255)

                                                                                   tGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAAt                                                                <  1:1270960/127‑1 (MQ=255)

                                                                                   tGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCAttt                                                   >  2:1036736/1‑140 (MQ=255)

                                                                                    gATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTa                                                  >  2:1328875/1‑140 (MQ=255)

                                                                                    gATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTa                                                  >  2:600112/1‑140 (MQ=255)

                                                                                        tgtgGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATa                                              <  1:644908/140‑1 (MQ=255)

                                                                                          tggtggCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAg                                            >  2:133164/1‑140 (MQ=255)

                                                                                          tggtggCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTT‑CGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAgg                                           >  1:867555/1‑140 (MQ=255)

                                                                                           ggtggCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAgg                                           <  1:133164/140‑1 (MQ=255)

                                                                                              ggCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGaa                                        >  2:159703/1‑140 (MQ=255)

                                                                                               gCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAAt                                       >  1:562532/1‑140 (MQ=255)

                                                                                                       ccAATATAGCCAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTcc                               <  1:159703/140‑1 (MQ=255)

                                                                                                                ccAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAgcgc                      >  2:218565/1‑140 (MQ=255)

                                                                                                                ccAATCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAgcgc                      >  1:820136/1‑140 (MQ=255)

                                                                                                                  aaTCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACGACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAt                    >  1:1357873/1‑140 (MQ=255)

                                                                                                                  aaTCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAt                    >  1:461014/1‑140 (MQ=255)

                                                                                                                    tCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCa                          >  2:161562/1‑132 (MQ=255)

                                                                                                                    tCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAttt                  >  2:1172572/1‑140 (MQ=255)

                                                                                                                    tCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAttt                  >  2:1407081/1‑140 (MQ=255)

                                                                                                                    tCTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAttt                  >  1:829557/1‑140 (MQ=255)

                                                                                                                    tCTTCAGCGTCTGTTTTTTT‑CGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAtttt                 <  2:413763/140‑1 (MQ=255)

                                                                                                                    tCTTCAGCGTCTGTTTTTTT‑CGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAtttt                 <  2:867555/140‑1 (MQ=255)

                                                                                                                     cTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAtttt                 >  2:342773/1‑140 (MQ=255)

                                                                                                                     cTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAtttt                 <  1:342773/140‑1 (MQ=255)

                                                                                                                     cTTCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAtttt                 >  2:1005889/1‑140 (MQ=255)

                                                                                                                     cTTCAGCGTCTGTCTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCAtttt                 <  2:829557/140‑1 (MQ=255)

                                                                                                                       tCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTc               <  1:1328875/140‑1 (MQ=255)

                                                                                                                       tCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTc               <  1:590608/140‑1 (MQ=255)

                                                                                                                       tCAGCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTc               <  1:600112/140‑1 (MQ=255)

                                                                                                                          gCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAgg            <  2:894452/140‑1 (MQ=255)

                                                                                                                          gCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAgg            <  2:1362242/140‑1 (MQ=255)

                                                                                                                          gCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAgg            <  2:1305869/140‑1 (MQ=255)

                                                                                                                          gCGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAgg            <  1:1005889/140‑1 (MQ=255)

                                                                                                                           cGTCTGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGt           <  1:503468/140‑1 (MQ=255)

                                                                                                                               tGTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATc       <  2:461014/140‑1 (MQ=255)

                                                                                                                                gTTTTTTTTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATcc      <  1:1407081/140‑1 (MQ=255)

                                                                                                                                    ttttTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGAc  <  1:307971/140‑1 (MQ=255)

                                                                                                                                    ttttTCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGAc  <  2:619211/140‑1 (MQ=255)

CATTTCAGGCACGTATTTGCGCACGGTTTCGGTAATATCATTCACCGTCACTTTCCCGGATAAATTAGCTTTATCCCAGCCCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGAC  >  NC_000913/4435930‑4436201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A