Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,439,105	T→C	N53S (AAC→AGC)	ytfJ ←	protein YtfJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,439,105	0	T	C	99.0%	374.2 / ‑4.9	104	N53S (AAC→AGC)	ytfJ	protein YtfJ
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (67/36); total (67/37)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 104 total aligned reads displayed.

GGTGGTGGTCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGC  >  NC_000913/4438969‑4439241

ggtggtggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTcc                                                                                                                                       >  2:319867/1‑140 (MQ=255)

ggtggtggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTcc                                                                                                                                       >  2:288761/1‑140 (MQ=255)

ggtggtggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTcc                                                                                                                                       >  2:879327/1‑140 (MQ=255)

  tggtggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCTACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAg                                                                                                                                     >  1:127592/1‑140 (MQ=255)

  tggtggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAg                                                                                                                                     >  2:1093781/1‑140 (MQ=255)

    gtggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGt                                                                                                                                   >  2:52969/1‑140 (MQ=255)

    gtggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGt                                                                                                                                   >  2:1091815/1‑140 (MQ=255)

     tggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGtt                                                                                                                                  >  1:251436/1‑140 (MQ=255)

          tGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTa                                                                                                                             >  2:695148/1‑140 (MQ=255)

           ggTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAg                                                                                                                            <  1:727388/140‑1 (MQ=255)

            gTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGc                                                                                                                           <  1:1091815/140‑1 (MQ=255)

            gTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGc                                                                                                                           >  2:432142/1‑140 (MQ=255)

                 gATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAAc                                                                                                                      >  2:822308/1‑140 (MQ=255)

                   tCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTg                                                                                                                    <  1:1174995/140‑1 (MQ=255)

                     gTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGAt                                                                                                                  <  2:922535/140‑1 (MQ=255)

                      tGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTaaa                                                                                                                       <  2:257592/134‑1 (MQ=255)

                      tGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATc                                                                                                                 >  2:1013689/1‑140 (MQ=255)

                      tGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATc                                                                                                                 >  2:479945/1‑140 (MQ=255)

                      tGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATc                                                                                                                 >  2:615576/1‑140 (MQ=255)

                      tGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATc                                                                                                                 >  1:1173260/1‑140 (MQ=255)

                      tGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATc                                                                                                                 >  2:1359192/1‑140 (MQ=255)

                       gCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCt                                                                                                                <  1:83652/140‑1 (MQ=255)

                       gCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGGCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCt                                                                                                                >  2:1066760/1‑140 (MQ=255)

                        cGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCtt                                                                                                               <  2:1173260/140‑1 (MQ=255)

                            aaCTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATc                                                                                                           <  2:438910/140‑1 (MQ=255)

                              cTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCaa                                                                                                         >  1:54567/1‑140 (MQ=255)

                                tCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGc                                                                                                       >  1:1503462/1‑140 (MQ=255)

                                 cGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCa                                                                                                      >  2:315775/1‑140 (MQ=255)

                                 cGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCa                                                                                                      <  1:315775/140‑1 (MQ=255)

                                  gccgATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCAc                                                                                                     <  2:1049045/137‑1 (MQ=255)

                                   cTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCAcc                                                                                                    >  2:493804/1‑140 (MQ=255)

                                     gATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCaa                                                                                                  >  1:1488814/1‑140 (MQ=255)

                                        ttAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCCATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTc                                                                                               <  2:1488814/140‑1 (MQ=255)

                                         tAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCg                                                                                              <  1:432142/140‑1 (MQ=255)

                                           aTCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGcc                                                                                            <  1:52969/140‑1 (MQ=255)

                                           aTCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGcc                                                                                            <  2:1503462/140‑1 (MQ=255)

                                              gCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCg                                                                                         <  1:493804/140‑1 (MQ=255)

                                                ttCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGAt                                                                                       >  2:1653177/1‑140 (MQ=255)

                                                ttCAATCAGCATCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGAt                                                                                       >  1:312506/1‑140 (MQ=255)

                                                         cGTCGCGTTTTTCTCTTTTACAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGc                                                                              >  1:1142656/1‑140 (MQ=255)

                                                          gTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGcc                                                                             >  2:1469982/1‑140 (MQ=255)

                                                          gTCGCGTTTTTCTCTTTTGCAGAAGCGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGcc                                                                             >  2:58513/1‑140 (MQ=255)

                                                            cgcgTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAAGTCGCCCCGATCGGTAATGCCag                                                                           >  1:1210703/1‑139 (MQ=255)

                                                                tttttCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATcgg                                                                       <  1:1013689/140‑1 (MQ=255)

                                                                   ttCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACACGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATcggcgg                                                                    <  2:373691/140‑1 (MQ=255)

                                                                      tctTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCAc                                                                 <  2:560421/140‑1 (MQ=255)

                                                                           tGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCt                                                            >  1:413650/1‑140 (MQ=255)

                                                                              agagGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGAc                                                         <  1:279409/140‑1 (MQ=255)

                                                                                  gTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGCTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGt                                                     >  1:225263/1‑140 (MQ=255)

                                                                                   tGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTCGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGtt                                                    <  1:1321287/140‑1 (MQ=255)

                                                                                     cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGtt                                                    >  2:1465/1‑138 (MQ=255)

                                                                                     cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGtt                                                    <  1:1465/138‑1 (MQ=255)

                                                                                      gACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCg                                                 <  1:479945/140‑1 (MQ=255)

                                                                                      gACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCg                                                 >  2:70279/1‑140 (MQ=255)

                                                                                            cAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGa                                           >  1:814771/1‑140 (MQ=255)

                                                                                            cAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGa                                           >  1:771188/1‑140 (MQ=255)

                                                                                                atGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGAtgtg                                       <  1:1093781/140‑1 (MQ=255)

                                                                                                   ttGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCgg                                    <  2:225263/140‑1 (MQ=255)

                                                                                                    tGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAGTCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGa                                   <  2:1210703/140‑1 (MQ=255)

                                                                                                    tGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGa                                   >  2:655592/1‑140 (MQ=255)

                                                                                                    tGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGa                                   <  1:615576/140‑1 (MQ=255)

                                                                                                    tGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGa                                   >  1:45458/1‑140 (MQ=255)

                                                                                                     gCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGaa                                  >  2:1608440/1‑140 (MQ=255)

                                                                                                      cAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAg                                 >  2:726590/1‑140 (MQ=255)

                                                                                                      cAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAg                                 >  2:1508000/1‑140 (MQ=255)

                                                                                                      cAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAg                                 >  2:454661/1‑140 (MQ=255)

                                                                                                      cAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAg                                 >  1:923998/1‑140 (MQ=255)

                                                                                                      cAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAg                                 >  2:1339031/1‑140 (MQ=255)

                                                                                                      cAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAg                                 >  1:1226217/1‑140 (MQ=255)

                                                                                                      cAGTACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAg                                 >  1:648164/1‑140 (MQ=255)

                                                                                                         tACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTg                                            >  1:1689036/1‑126 (MQ=255)

                                                                                                         tACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTg                                            <  2:1689036/126‑1 (MQ=255)

                                                                                                         tACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCca                              >  1:611582/1‑140 (MQ=255)

                                                                                                         tACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCca                              >  2:386295/1‑140 (MQ=255)

                                                                                                         tACTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCca                              >  1:682136/1‑140 (MQ=255)

                                                                                                           cTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatc                            >  1:1328833/1‑140 (MQ=255)

                                                                                                           cTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatc                            <  1:1339031/140‑1 (MQ=255)

                                                                                                           cTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatc                            >  1:410642/1‑140 (MQ=255)

                                                                                                           cTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatc                            >  2:924580/1‑140 (MQ=255)

                                                                                                           cTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatc                            >  2:1211848/1‑140 (MQ=255)

                                                                                                           cTCGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTATAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatc                            >  1:243747/1‑140 (MQ=255)

                                                                                                             cGCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatcat                          >  2:1062276/1‑140 (MQ=255)

                                                                                                              gCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCatcatc                         >  2:705482/1‑140 (MQ=255)

                                                                                                              gCACTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCatcatc                         >  1:1590042/1‑140 (MQ=255)

                                                                                                                 cTTTTCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGc                      <  1:655592/140‑1 (MQ=255)

                                                                                                                   tttCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCgg                       >  1:370956/1‑137 (MQ=255)

                                                                                                                   tttCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCgg                       <  2:370956/137‑1 (MQ=255)

                                                                                                                   tttCCCACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCaa                    >  1:1478179/1‑140 (MQ=255)

                                                                                                                      cccACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAAca                  <  2:1323789/139‑1 (MQ=255)

                                                                                                                      cccACTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAAca                  >  1:1323789/1‑139 (MQ=255)

                                                                                                                          cTAACTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAgcagg             <  1:879327/140‑1 (MQ=255)

                                                                                                                              cTGCGTGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGcaggcagg         >  1:618113/1‑140 (MQ=255)

                                                                                                                              cTGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGTCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGcaggcagg         >  1:1381566/1‑140 (MQ=255)

                                                                                                                               tGCGCGCTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGt        <  1:1653177/140‑1 (MQ=255)

                                                                                                                                 cgcgcTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAgcaggcag          <  2:1518447/136‑1 (MQ=255)

                                                                                                                                 cgcgcTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAgcaggcag          >  1:1518447/1‑136 (MQ=255)

                                                                                                                                 cgcgcTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGa      >  1:42664/1‑140 (MQ=255)

                                                                                                                                   cgcTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAAca                  >  2:677184/1‑126 (MQ=255)

                                                                                                                                   cgcTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAAca                  <  1:677184/126‑1 (MQ=255)

                                                                                                                                     cTGCTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGc  <  2:1328833/140‑1 (MQ=255)

GGTGGTGGTCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGC  >  NC_000913/4438969‑4439241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A