Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,484,244 T→C T13A (ACC→GCC)  holC ← DNA polymerase III subunit chi

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,484,2440TC100.0% 275.7 / NA 75T13A (ACC→GCC) holCDNA polymerase III subunit chi
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/50);  total (25/50)

CCCACAGGGCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGTGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG  >  NC_000913/4484109‑4484378
                                                                                                                                       |                                                                                                                                      
cccACAGGGCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCa                                                                                                                                    <  2:99487/140‑1 (MQ=255)
cccACAGGGCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCa                                                                                                                                    <  2:75562/140‑1 (MQ=255)
cccACAGGGCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCa                                                                                                                                    <  1:759994/140‑1 (MQ=255)
  cacaGGGCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCAtt                                                                                                                                  <  2:709532/140‑1 (MQ=255)
  cacaGGGCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCAtt                                                                                                                                  <  1:556280/140‑1 (MQ=255)
       ggCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCa                                                                                                                             <  2:519728/140‑1 (MQ=255)
       ggCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCa                                                                                                                             <  2:1239529/140‑1 (MQ=255)
       ggCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCa                                                                                                                             <  2:122326/140‑1 (MQ=255)
       ggCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCa                                                                                                                             <  1:1170825/140‑1 (MQ=255)
         cTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGa                                                                                                                           >  1:926905/1‑140 (MQ=255)
         cTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGa                                                                                                                           <  1:179779/140‑1 (MQ=255)
         cTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGa                                                                                                                           >  1:17516/1‑140 (MQ=255)
           tCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAg                                                                                                                         >  1:104011/1‑140 (MQ=255)
           tCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAg                                                                                                                         >  2:890228/1‑140 (MQ=255)
               ccAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAg                                                                                                                     >  1:1078781/1‑140 (MQ=255)
                 aGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTc                                                                                                                               >  1:797281/1‑128 (MQ=255)
                 aGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTc                                                                                                                               <  2:797281/128‑1 (MQ=255)
                   ccGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACg                                                                                                                 <  2:459545/140‑1 (MQ=255)
                   ccGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACg                                                                                                                 >  1:459545/1‑140 (MQ=255)
                            cTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGtttt                                                                                                        <  1:1261820/140‑1 (MQ=255)
                             tGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGttttt                                                                                                       <  1:281287/140‑1 (MQ=255)
                                    cATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAAt                                                                                                <  1:873647/140‑1 (MQ=255)
                                    cATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAAt                                                                                                <  1:146177/140‑1 (MQ=255)
                                     aTCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATg                                                                                               >  2:74893/1‑140 (MQ=255)
                                       cTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATggg                                                                                             <  1:729921/140‑1 (MQ=255)
                                        ttCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATgggg                                                                                            <  1:890228/140‑1 (MQ=255)
                                         tCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGc                                                                                           <  2:879749/140‑1 (MQ=255)
                                          cacaGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGct                                                                                          >  1:640230/1‑140 (MQ=255)
                                                 gATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTtgtg                                                                                   <  1:1151107/140‑1 (MQ=255)
                                                 gATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTtgtg                                                                                   <  2:17516/140‑1 (MQ=255)
                                                 gATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTtgtg                                                                                   <  2:266323/140‑1 (MQ=255)
                                                 gATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTtgtg                                                                                   <  2:411489/140‑1 (MQ=255)
                                                    gAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTgtggtg                                                                                >  1:573112/1‑140 (MQ=255)
                                                         cgcgCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTGATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGAttt                                                                           >  2:1238402/1‑140 (MQ=255)
                                                         cgcgCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGAttt                                                                           >  2:1072925/1‑140 (MQ=255)
                                                              ttACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATa                                                                      <  1:210320/140‑1 (MQ=255)
                                                              ttACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATa                                                                      <  2:467231/140‑1 (MQ=255)
                                                                aCCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACg                                                                    >  1:873395/1‑140 (MQ=255)
                                                                 ccGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGc                                                                   <  1:662855/140‑1 (MQ=255)
                                                                 ccGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGc                                                                   <  1:452891/140‑1 (MQ=255)
                                                                   gCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCAt                                                                 <  1:1337703/140‑1 (MQ=255)
                                                                   gCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCAt                                                                 <  1:83334/140‑1 (MQ=255)
                                                                    cTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCa                                                                  >  2:723345/1‑138 (MQ=255)
                                                                    cTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCa                                                                  <  1:723345/138‑1 (MQ=255)
                                                                      gcgcCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAg                                                              >  1:1380355/1‑140 (MQ=255)
                                                                      gcgcCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAg                                                              <  2:284955/140‑1 (MQ=255)
                                                                      gcgcCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAg                                                              >  1:1164693/1‑140 (MQ=255)
                                                                                  tGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCa                                                  <  1:1162669/140‑1 (MQ=255)
                                                                                    cGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAgc                                                >  1:1125822/1‑140 (MQ=255)
                                                                                      gCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAgcgc                                              <  2:646214/140‑1 (MQ=255)
                                                                                         aTTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTg                                           <  2:1139089/140‑1 (MQ=255)
                                                                                               cacacCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGa                                     <  2:926905/140‑1 (MQ=255)
                                                                                               cacacCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGa                                     <  1:1007101/140‑1 (MQ=255)
                                                                                                acacCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGAc                                    <  2:104011/140‑1 (MQ=255)
                                                                                                 cacCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACg                                   <  2:1391508/140‑1 (MQ=255)
                                                                                                 cacCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACg                                   <  1:1381926/140‑1 (MQ=255)
                                                                                                    cAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCtg                                >  1:828837/1‑140 (MQ=255)
                                                                                                    cAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCtg                                <  2:573112/140‑1 (MQ=255)
                                                                                                     aGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCtgt                               <  1:1072925/140‑1 (MQ=255)
                                                                                                     aGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCtgt                               >  1:412727/1‑140 (MQ=255)
                                                                                                      gTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCtgt                               >  2:289489/1‑139 (MQ=255)
                                                                                                      gTTGCTCAACGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCtgt                               <  1:289489/139‑1 (MQ=255)
                                                                                                              aCGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTa                      >  2:795743/1‑140 (MQ=255)
                                                                                                              aCGGCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTa                      >  1:525201/1‑140 (MQ=255)
                                                                                                                ggCGCTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATc                    <  2:1110071/140‑1 (MQ=255)
                                                                                                                 gcgcTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCa                   <  2:1380355/140‑1 (MQ=255)
                                                                                                                  cgcTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAg                  >  2:22953/1‑140 (MQ=255)
                                                                                                                  cgcTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAg                  >  1:773125/1‑140 (MQ=255)
                                                                                                                  cgcTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAg                  >  1:1389060/1‑140 (MQ=255)
                                                                                                                   gcTTAAGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTAATCAgg                 <  2:873395/140‑1 (MQ=255)
                                                                                                                        aGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTAc            <  2:1125822/140‑1 (MQ=255)
                                                                                                                        aGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTAc            <  1:22953/140‑1 (MQ=255)
                                                                                                                        aGCCATCGACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGGTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTAc            <  2:773125/140‑1 (MQ=255)
                                                                                                                               gACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCa     <  2:718464/140‑1 (MQ=255)
                                                                                                                               gACGGTGGCGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCa     <  1:903873/140‑1 (MQ=255)
                                                                                                                                  ggtggcGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTg  <  1:795743/140‑1 (MQ=255)
                                                                                                                                  ggtggcGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTg  <  1:74893/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CCCACAGGGCTTCATCCAGCCGGTAAGCCTGCTTTTCATCTTCACAGGCGATGAGCACGCGCTTACCGCTGCGCCAACGTTCTGCGGCAATTTCACACACCAGTTGCTCAACGGCGCTTAAGCCATCGACGGTGGTGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTG  >  NC_000913/4484109‑4484378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A