Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	113,734	A→G	K97K (AAA→AAG)	guaC →	GMP reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	113,734	0	A	G	100.0%	167.1 / NA	47	K97K (AAA→AAG)	guaC	GMP reductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/21); total (26/21)

ATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTCCGAACACTTCGTG  >  NC_000913/113601‑113860

atatGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCAtgtg                                                                                                                          <  2:654276/140‑1 (MQ=255)

 tatGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGa                                                                                                                         <  1:444764/140‑1 (MQ=255)

 tatGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGa                                                                                                                         <  1:450514/140‑1 (MQ=255)

     gACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGt                                                                                                                     <  1:603739/140‑1 (MQ=255)

          cGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTa                                                                                                                >  2:231190/1‑140 (MQ=255)

            tAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCAtgtg                                                                                                                          <  2:533126/128‑1 (MQ=255)

            tAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCAtgtg                                                                                                                          >  1:533126/1‑128 (MQ=255)

              ggCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCgg                                                                                                            <  1:578580/140‑1 (MQ=255)

               gCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGt                                                                                                           <  1:441677/140‑1 (MQ=255)

                  caTTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACg                                                                                                        >  2:174747/1‑140 (MQ=255)

                   aTTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGt                                                                                                       <  1:787630/140‑1 (MQ=255)

                    ttttCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTc                                                                                                      >  1:537241/1‑140 (MQ=255)

                         tATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATg                                                                                                 <  2:619730/140‑1 (MQ=255)

                                  tGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCg                                                                                        >  1:216832/1‑140 (MQ=255)

                                  tGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCg                                                                                        >  1:839695/1‑140 (MQ=255)

                                   gcgcTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGa                                                                                       <  2:502740/140‑1 (MQ=255)

                                                   atatTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGAtt                                                                       >  2:851792/1‑140 (MQ=255)

                                                     atTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATtct                                                                     <  1:851792/140‑1 (MQ=255)

                                                                  tgCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGAc                                                                 <  1:399188/131‑1 (MQ=255)

                                                                  tgCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGAc                                                                 >  2:399188/1‑131 (MQ=255)

                                                                  tgCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCg                                                        >  2:748440/1‑140 (MQ=255)

                                                                  tgCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCg                                                        >  2:852063/1‑140 (MQ=255)

                                                                         acacTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTaa                                                 >  1:592133/1‑140 (MQ=255)

                                                                            cTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCTGGCATTAAACt                                              >  2:335311/1‑140 (MQ=255)

                                                                            cTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACt                                              >  1:829050/1‑140 (MQ=255)

                                                                            cTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACt                                              >  2:676411/1‑140 (MQ=255)

                                                                            cTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACt                                              >  2:204741/1‑140 (MQ=255)

                                                                              aTTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTc                                            <  2:839695/140‑1 (MQ=255)

                                                                              aTTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTc                                            >  2:391427/1‑140 (MQ=255)

                                                                               ttCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCg                                           >  2:866981/1‑140 (MQ=255)

                                                                                tCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGt                                          <  1:204741/140‑1 (MQ=255)

                                                                                 cTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGtt                                         <  1:391427/140‑1 (MQ=255)

                                                                                     cGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTa                                     >  1:144560/1‑140 (MQ=255)

                                                                                       aaGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTAtt                                   >  1:199907/1‑140 (MQ=255)

                                                                                         gagTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGa                                 >  2:485798/1‑140 (MQ=255)

                                                                                            tGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGt                              >  2:414519/1‑140 (MQ=255)

                                                                                              gCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTgg                            >  2:529573/1‑140 (MQ=255)

                                                                                              gCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTgg                            >  2:656704/1‑140 (MQ=255)

                                                                                                 aGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGa                         <  1:676411/140‑1 (MQ=255)

                                                                                                     tttATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATgg                     <  2:289482/140‑1 (MQ=255)

                                                                                                     tttATCAACAATTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATgg                     >  1:440498/1‑140 (MQ=255)

                                                                                                              aaTTCTTCCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTCCGa            <  1:866981/140‑1 (MQ=255)

                                                                                                                ttcttcCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTCCGAac          <  1:852063/140‑1 (MQ=255)

                                                                                                                   ttcCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTCCGAACACt       <  1:540691/140‑1 (MQ=255)

                                                                                                                   ttcCGCTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTCCGAACACt       <  1:231190/140‑1 (MQ=255)

                                                                                                                        cTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAAGAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTCCGAACACTTCGTg  >  2:785583/1‑140 (MQ=255)

                                                                                                                        cTGATGTGCTGAAGCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTCCGAACACTTCGTg  >  1:286235/1‑140 (MQ=255)

ATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTCCGAACACTTCGTG  >  NC_000913/113601‑113860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A