Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	113,959	T→C	G172G (GGT→GGC)	guaC →	GMP reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	113,959	0	T	C	100.0%	158.6 / NA	45	G172G (GGT→GGC)	guaC	GMP reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/21); total (24/21)

ACGTGGCGAATGGTTATTCCGAACACTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGTGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCG  >  NC_000913/113829‑114092

aCGTGGCGAATGGTTATTCCGAACACTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGa                                                                                                                                  >  1:856647/1‑136 (MQ=255)

aCGTGGCGAATGGTTATTCCGAACACTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGa                                                                                                                                  <  2:856647/136‑1 (MQ=255)

  gTGGCGAATGGTTATTCCGAACACTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGt                                                                                                                            <  1:129231/140‑1 (MQ=255)

     gCGAATGGTTATTCCAAACACTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTaa                                                                                                                         >  1:713295/1‑140 (MQ=255)

               aTTCCGAACACTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCAtt                                                                                                               <  2:564026/140‑1 (MQ=255)

               aTTCCGAACACTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCAtt                                                                                                               >  1:441677/1‑140 (MQ=255)

                    gAACACTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGccc                                                                                                          <  1:39283/140‑1 (MQ=255)

                     aaCACTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCa                                                                                                         >  2:603942/1‑140 (MQ=255)

                        acTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGt                                                                                                      <  2:319817/140‑1 (MQ=255)

                          ttCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTc                                                                                                    >  1:552926/1‑140 (MQ=255)

                            cGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTg                                                                                                  <  1:603942/140‑1 (MQ=255)

                                   ttCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTa                                                                                            <  2:282788/139‑1 (MQ=255)

                                   ttCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTa                                                                                            >  1:282788/1‑139 (MQ=255)

                                   ttCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACCGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTAc                                                                                           >  2:1624/1‑140 (MQ=255)

                                       ttGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACCGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACt                                                                                       <  1:1624/140‑1 (MQ=255)

                                        tGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTc                                                                                      >  2:202695/1‑140 (MQ=255)

                                                  cgTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAAc                                                                            >  2:586876/1‑140 (MQ=255)

                                                   gTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACa                                                                           <  1:390957/140‑1 (MQ=255)

                                                           tGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCgg                                                                   >  2:92786/1‑140 (MQ=255)

                                                               cGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCaa                                                                               >  1:83353/1‑124 (MQ=255)

                                                               cGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCaa                                                                               <  2:83353/124‑1 (MQ=255)

                                                                 aCCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGTAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATcc                                                             >  2:736341/1‑140 (MQ=255)

                                                                            ttGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTg                                                  <  1:202695/140‑1 (MQ=255)

                                                                            ttGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTg                                                  <  2:261711/140‑1 (MQ=255)

                                                                                  tGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTaa                                            >  2:33034/1‑140 (MQ=255)

                                                                                        cGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAAt                                      >  1:415656/1‑140 (MQ=255)

                                                                                         gTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAAtg                                     >  1:832717/1‑140 (MQ=255)

                                                                                         gTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAAtg                                     >  1:879371/1‑140 (MQ=255)

                                                                                             tGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGcc                                 >  2:454425/1‑140 (MQ=255)

                                                                                              gACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCg                                >  2:322670/1‑140 (MQ=255)

                                                                                              gACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCg                                <  2:415656/140‑1 (MQ=255)

                                                                                              gACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCg                                <  1:322670/140‑1 (MQ=255)

                                                                                                cTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGAt                              <  1:33034/140‑1 (MQ=255)

                                                                                                   gTGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGAtgct                           >  1:203008/1‑140 (MQ=255)

                                                                                                    tGAAATGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGAtgctg                          >  2:76928/1‑140 (MQ=255)

                                                                                                      aaaTGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTgcg                        <  1:92786/140‑1 (MQ=255)

                                                                                                       aaTGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTgcgc                       <  2:203008/140‑1 (MQ=255)

                                                                                                       aaTGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTgcgc                       <  2:832717/140‑1 (MQ=255)

                                                                                                        aTGTGTGAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCa                      <  1:76928/140‑1 (MQ=255)

                                                                                                            gtgAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGt                  <  2:713295/140‑1 (MQ=255)

                                                                                                             tgAGGAGCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTc                 >  2:77089/1‑140 (MQ=255)

                                                                                                                 gagCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTggg             >  1:433375/1‑140 (MQ=255)

                                                                                                                   gCTTATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCg           <  1:736341/140‑1 (MQ=255)

                                                                                                                     ttATCCTCTCAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGa         >  2:636685/1‑140 (MQ=255)

                                                                                                                            ctcAGGCGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGCGCCGATGCTGCGCACGGTCTGGGCGGAATGATCg  >  1:534236/1‑140 (MQ=255)

ACGTGGCGAATGGTTATTCCGAACACTTCGTGCAGTTCGTTGCGAAAGCGCGTGAAGCGTGGCCGACCAAAACCATTTGTGCTGGTAACGTAGTGACTGGTGAAATGTGTGAGGAGCTTATCCTCTCAGGTGCCGATATCGTTAAAGTTGGCATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCG  >  NC_000913/113829‑114092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A