Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	118,308	A→G	V113A (GTG→GCG)	nadC ←	quinolinate phosphoribosyltransferase (decarboxylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	118,308	0	A	G	100.0%	197.6 / NA	56	V113A (GTG→GCG)	nadC	quinolinate phosphoribosyltransferase (decarboxylating)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/25); total (31/25)

GTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAA  >  NC_000913/118177‑118443

gTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGAt                                                                                                                                 <  1:606643/140‑1 (MQ=255)

         tGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCa                                                                                                                        >  2:971284/1‑140 (MQ=255)

                          tGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGgattg                                                                                                       >  2:1128874/1‑140 (MQ=255)

                          tGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGgattg                                                                                                       >  1:383470/1‑140 (MQ=255)

                            cGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGgattgat                                                                                                     >  2:498014/1‑140 (MQ=255)

                            cGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGgattgat                                                                                                     >  2:40406/1‑140 (MQ=255)

                            cGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGgattgat                                                                                                     >  2:249408/1‑140 (MQ=255)

                            cGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGgattgat                                                                                                     >  2:108539/1‑140 (MQ=255)

                            cGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGgattgat                                                                                                     >  1:556503/1‑140 (MQ=255)

                                 tGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCa                                                                                                <  1:971284/140‑1 (MQ=255)

                                      ccTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATtgat                                                                                           <  2:258437/140‑1 (MQ=255)

                                        ttCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATtgatga                                                                                         >  1:469819/1‑140 (MQ=255)

                                         tCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGattgattg                                                                                                   <  1:833572/129‑1 (MQ=255)

                                         tCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGattgattg                                                                                                   >  2:833572/1‑129 (MQ=255)

                                          ccAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACa                                                                                       <  1:1043035/140‑1 (MQ=255)

                                           cAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGTGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACAt                                                                                      <  1:637290/140‑1 (MQ=255)

                                           cAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACAt                                                                                      <  1:1128874/140‑1 (MQ=255)

                                           cAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACAt                                                                                      <  1:78850/140‑1 (MQ=255)

                                              cAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGc                                                                                   >  2:457070/1‑140 (MQ=255)

                                                 ttCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTgg                                                                                                  <  1:629538/122‑1 (MQ=255)

                                                 ttCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTgg                                                                                                  >  2:629538/1‑122 (MQ=255)

                                                 ttCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGt                                                                                <  1:465896/140‑1 (MQ=255)

                                                   cGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCg                                                                                    <  1:621258/134‑1 (MQ=255)

                                                   cGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCg                                                                                    >  2:621258/1‑134 (MQ=255)

                                                      aaTAGTGGCGTCCCTTCCTGGCACCTCCTGAAAGGGTTTGCACAAAATTAAGCCCAGGGCGTCCGCCCGTTACCGGCGCGCGGGAT‑GGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCca                                                                          <  2:232909/139‑1 (MQ=255)

                                                       aTAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCca                                                                          <  1:457070/140‑1 (MQ=255)

                                                           tGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATg                                                                      <  2:469819/140‑1 (MQ=255)

                                                                tACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGa                                                                 <  2:522638/140‑1 (MQ=255)

                                                                 aCCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGAt                                                                <  1:249408/140‑1 (MQ=255)

                                                                     tACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATg                                                            >  2:85570/1‑140 (MQ=255)

                                                                     tACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATg                                                            >  2:936907/1‑140 (MQ=255)

                                                                      aCTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATgg                                                           >  1:517997/1‑140 (MQ=255)

                                                                        tGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTg                                                         <  2:538086/140‑1 (MQ=255)

                                                                             aCTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATg                                                                      <  1:764303/122‑1 (MQ=255)

                                                                             aCTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATg                                                                      >  2:764303/1‑122 (MQ=255)

                                                                             aCTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtc                                                    >  2:976521/1‑140 (MQ=255)

                                                                             aCTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtc                                                    >  2:735818/1‑140 (MQ=255)

                                                                                    aaaGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCt                                             >  2:122388/1‑140 (MQ=255)

                                                                                      aGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGc                                           <  2:127949/140‑1 (MQ=255)

                                                                                      aGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGc                                           <  1:122388/140‑1 (MQ=255)

                                                                                         gTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCt                                             >  1:439197/1‑135 (MQ=255)

                                                                                         gTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCt                                             <  2:439197/135‑1 (MQ=255)

                                                                                         gTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAg                                        >  1:1079322/1‑140 (MQ=255)

                                                                                         gTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAg                                        >  2:113197/1‑140 (MQ=255)

                                                                                            tGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTg                                     >  1:78427/1‑140 (MQ=255)

                                                                                                caAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                                 <  2:1079322/140‑1 (MQ=255)

                                                                                                  aaaTTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCTCATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATaaa                               <  1:136202/140‑1 (MQ=255)

                                                                                                  aaaTTAAGCGCAGTGCGTTCGCCCG‑TAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAac                              >  1:240234/1‑140 (MQ=255)

                                                                                                   aaTTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAac                              >  1:66711/1‑140 (MQ=255)

                                                                                                    aTTAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAaca                             >  2:633399/1‑140 (MQ=255)

                                                                                                     ttAAGCGCAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAacac                            <  1:976521/140‑1 (MQ=255)

                                                                                                          cgcAGTGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCtt                       >  1:227372/1‑140 (MQ=255)

                                                                                                               tGCGTTCGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAAcc                  >  2:114730/1‑140 (MQ=255)

                                                                                                                     cGCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGt            >  1:581756/1‑140 (MQ=255)

                                                                                                                      gCCCGTTAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGtt           <  1:114730/140‑1 (MQ=255)

                                                                                                                           ttAACAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCg      >  2:937016/1‑140 (MQ=255)

                                                                                                                               cAGCGCGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCaaa  <  2:581756/140‑1 (MQ=255)

GTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGCGTTTGCCGCAAA  >  NC_000913/118177‑118443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A