Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,529,416	T→G	pseudogene (1527/2049 nt)	rhsE →	RhsE protein in rhs element

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,529,416	0	T	G	98.6%	238.2 / ‑4.2	69	pseudogene (1527/2049 nt)	rhsE	RhsE protein in rhs element
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (31/37); total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

TGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTATGCCGTATCTGGTGTACCTGGATTGA  >  NC_000913/1529281‑1529552

tGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGt                                                                                                                                      >  2:389830/1‑140 (MQ=255)

         cccGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGtg                                                                                                                             <  1:101186/140‑1 (MQ=255)

         cccGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGtg                                                                                                                             >  1:279094/1‑140 (MQ=255)

           cGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGtgga                                                                                                                           <  1:458798/140‑1 (MQ=255)

           cGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGtgga                                                                                                                           <  1:482078/140‑1 (MQ=255)

            gCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGtggat                                                                                                                          <  1:280749/140‑1 (MQ=255)

             cATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGtggatg                                                                                                                         <  2:279094/140‑1 (MQ=255)

             cATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGt                                                                                                                              >  2:108645/1‑135 (MQ=255)

             cATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGt                                                                                                                              <  1:108645/135‑1 (MQ=255)

              aTCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCA‑CCCAGACCCGATGGGGTTGAGAGGtggatgga                                                                                                                       >  1:234057/1‑140 (MQ=255)

               tCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGtggatgga                                                                                                                       <  2:104570/140‑1 (MQ=255)

               tCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGtggatgga                                                                                                                       <  2:237249/140‑1 (MQ=255)

                 aCCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAat                                                                                                                     <  1:614091/140‑1 (MQ=255)

                     gCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGtgga                                                                                                                           >  1:83694/1‑130 (MQ=255)

                     gCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGtgga                                                                                                                           <  2:83694/130‑1 (MQ=255)

                        ccAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATc                                                                                                              <  2:127249/140‑1 (MQ=255)

                          aGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAg                                                                                                            >  2:560105/1‑140 (MQ=255)

                           gCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGt                                                                                                           >  2:585406/1‑140 (MQ=255)

                           gCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGCGGATGGAATATGTATCAGt                                                                                                           >  2:536643/1‑140 (MQ=255)

                            ccGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAat                                                                                                                     <  2:873182/129‑1 (MQ=255)

                            ccGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAat                                                                                                                     >  1:873182/1‑129 (MQ=255)

                             cGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTAt                                                                                                         <  2:475103/140‑1 (MQ=255)

                                    tCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCA‑CCCAGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGaa                                                                                                 <  2:234057/140‑1 (MQ=255)

                                     cTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGaa                                                                                                 <  1:375009/140‑1 (MQ=255)

                                     cTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGaa                                                                                                 <  2:576905/140‑1 (MQ=255)

                                       gCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATc                                                                                               <  2:249222/140‑1 (MQ=255)

                                         cAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATccc                                                                                             <  1:585406/140‑1 (MQ=255)

                                         cAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATccc                                                                                             >  2:618173/1‑140 (MQ=255)

                                            ggCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATa                                                                                          >  2:747128/1‑140 (MQ=255)

                                            ggCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATa                                                                                          >  2:566592/1‑140 (MQ=255)

                                             gcagcagcaTGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATAc                                                                                         <  2:134358/140‑1 (MQ=255)

                                             gcagcagcaTGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATAc                                                                                         <  1:104333/140‑1 (MQ=255)

                                              cagcagcaTGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACa                                                                                        >  1:21446/1‑140 (MQ=255)

                                                        atgaGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGAc                                                                              <  1:560105/140‑1 (MQ=255)

                                                           aggagTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCa                                                                           <  1:536643/140‑1 (MQ=255)

                                                             gagTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAAt                                                                         <  1:389830/140‑1 (MQ=255)

                                                              agTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATg                                                                        >  1:212180/1‑140 (MQ=255)

                                                                 cGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATgggg                                                                     <  1:790276/140‑1 (MQ=255)

                                                                 cGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATgggg                                                                     >  2:675854/1‑140 (MQ=255)

                                                                 cGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATgggg                                                                     <  1:618173/140‑1 (MQ=255)

                                                                  ggggCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGa                                                                               >  1:787266/1‑129 (MQ=255)

                                                                  ggggCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGa                                                                               <  2:787266/129‑1 (MQ=255)

                                                                      cTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGa                                                                <  1:742484/140‑1 (MQ=255)

                                                                        gTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCGCCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATg                                                              <  1:61587/140‑1 (MQ=255)

                                                                        gTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATg                                                              <  2:212180/140‑1 (MQ=255)

                                                                        gTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATg                                                              <  1:420496/140‑1 (MQ=255)

                                                                             ataACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGAtt                                                         <  1:866557/140‑1 (MQ=255)

                                                                              taACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTg                                                        >  2:736727/1‑140 (MQ=255)

                                                                              taACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTg                                                        >  2:6119/1‑140 (MQ=255)

                                                                                   gTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATg                                                              >  2:251754/1‑129 (MQ=255)

                                                                                   gTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATg                                                              <  1:251754/129‑1 (MQ=255)

                                                                                   gTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAat                                                   >  2:35879/1‑140 (MQ=255)

                                                                                   gTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAat                                                   >  1:684461/1‑140 (MQ=255)

                                                                                   gTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAat                                                   >  2:501691/1‑140 (MQ=255)

                                                                                     cACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAatat                                                 >  2:713845/1‑140 (MQ=255)

                                                                                     cACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAatat                                                 >  1:646774/1‑140 (MQ=255)

                                                                                      aCCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATg                                                >  1:152719/1‑140 (MQ=255)

                                                                                      aCCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATg                                                >  1:724830/1‑140 (MQ=255)

                                                                                            aCTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCa                                          <  2:677865/140‑1 (MQ=255)

                                                                                              tACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAgg                                        <  1:300808/140‑1 (MQ=255)

                                                                                                     cGTTGCAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTa                                 >  1:474626/1‑140 (MQ=255)

                                                                                                          cAGGGGCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTa                            >  1:524158/1‑140 (MQ=255)

                                                                                                            ggggCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTATg                          >  1:264853/1‑140 (MQ=255)

                                                                                                             gggCGGTATATCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTATGc                         <  1:713845/140‑1 (MQ=255)

                                                                                                                     tatCACCCCGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTATGCCGTATCTg                 <  1:501691/140‑1 (MQ=255)

                                                                                                                           cccGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTATGCCGTATCTGGTGTAc           <  2:160048/140‑1 (MQ=255)

                                                                                                                             cGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTATGCCGTATCTGGTGTACCt         >  2:59817/1‑140 (MQ=255)

                                                                                                                             cGGACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTATGCCGTATCTGGTGTACCt         >  1:185836/1‑140 (MQ=255)

                                                                                                                               gACCCGATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTATGCCGTATCTGGTGTACCTgg       >  2:836024/1‑140 (MQ=255)

                                                                                                                                    gATGGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTATGCCGTATCTGGTGTACCTGgattga  <  2:21446/140‑1 (MQ=255)

TGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGACATCAGGTGGACTAATTTATGCCGTATCTGGTGTACCTGGATTGA  >  NC_000913/1529281‑1529552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A