Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,555,035	C→T	G212D (GGC→GAC)	maeA ←	malate dehydrogenase, NAD‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,555,035	0	C	T	100.0%	125.5 / NA	37	G212D (GGC→GAC)	maeA	malate dehydrogenase, NAD‑requiring
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/21); total (16/21)

GGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGAT  >  NC_000913/1554906‑1555174

ggCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAg                                                                                                                                   <  1:788208/140‑1 (MQ=255)

  cATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCg                                                                                                                                 <  2:827620/140‑1 (MQ=255)

    tCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGa                                                                                                                               >  2:140324/1‑140 (MQ=255)

    tCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGa                                                                                                                               <  1:66922/140‑1 (MQ=255)

    tCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGa                                                                                                                               <  1:670670/140‑1 (MQ=255)

    tCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCAAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGa                                                                                                                               <  1:838244/140‑1 (MQ=255)

          ttttttGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTa                                                                                                                         <  2:546537/140‑1 (MQ=255)

            ttttGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAag                                                                                                                       <  1:514755/140‑1 (MQ=255)

            ttttGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAag                                                                                                                       >  2:593647/1‑140 (MQ=255)

                                 tGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTcc                                                                                                  >  1:351817/1‑140 (MQ=255)

                                 tGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTcc                                                                                                  <  1:135856/140‑1 (MQ=255)

                                  gCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATATATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCg                                                                                                 >  2:523508/1‑140 (MQ=255)

                                   cAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGa                                                                                                <  1:400582/140‑1 (MQ=255)

                                                 gCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCaccacc                                                                                  >  1:59905/1‑140 (MQ=255)

                                                    aGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGa                                                                               <  2:320265/140‑1 (MQ=255)

                                                    aGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGa                                                                               <  1:416241/140‑1 (MQ=255)

                                                      cGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAg                                                                             >  1:658518/1‑140 (MQ=255)

                                                      cGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAg                                                                             >  1:258319/1‑140 (MQ=255)

                                                      cGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAg                                                                             >  1:18730/1‑140 (MQ=255)

                                                                caGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTACATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGcc                                                                   >  2:654261/1‑140 (MQ=255)

                                                                   ccTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCggg                                                                >  1:761261/1‑140 (MQ=255)

                                                                       gATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGa                                                            >  1:860574/1‑140 (MQ=255)

                                                                             ttCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATgccgc                                                      >  1:747510/1‑140 (MQ=255)

                                                                              tcatcaACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATgccgcc                                                     <  1:523508/140‑1 (MQ=255)

                                                                                   aaCGAATTCATAGTATTCGTCGTCATTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAgg                                                <  2:18730/140‑1 (MQ=255)

                                                                                   aaCGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAgg                                                <  2:351817/140‑1 (MQ=255)

                                                                                                tATTCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGa                                   <  1:352590/140‑1 (MQ=255)

                                                                                                  ttCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGc                                               <  1:760242/126‑1 (MQ=255)

                                                                                                  ttCGTCGTCAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGc                                               >  2:760242/1‑126 (MQ=255)

                                                                                                     gtcgtcAGTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGtt                              >  1:423990/1‑140 (MQ=255)

                                                                                                            gTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGAt                       <  1:501208/140‑1 (MQ=255)

                                                                                                            gTGATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGAt                       <  2:747510/140‑1 (MQ=255)

                                                                                                            gTGATACGCGGATTACGCCAGTACATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGAt                       <  1:654261/140‑1 (MQ=255)

                                                                                                             tGATACGCGGATTACGCCAGTCCATATACAGCGGATCGATAAACAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGCATAGAGCGACAGGTTACCGATc                      >  2:657013/1‑140 (MQ=255)

                                                                                                              gATACGCGGATTACGCCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCg                     <  1:145210/140‑1 (MQ=255)

                                                                                                                       aTTACACCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGa                        <  1:773978/128‑1 (MQ=255)

                                                                                                                       aTTACACCAGTCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGa                        >  2:773978/1‑128 (MQ=255)

                                                                                                                              cAGCCCATATACAGCGGATGGTAAAGCAGCTGTTGGTTGTTCGTCCCGACACCCAGCCCCCCGGGAGGGGTATCCGCGGGGCTGAGGCCGCCACAGGCGGTATAGAGCGACAGTTTCCCGATCGAAATGCCCACCccgcc     <  2:59905/140‑1 (MQ=255)

                                                                                                                                 cccATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGAt  >  1:679185/1‑140 (MQ=255)

GGCATCGCATTTTTTTGAGCAAAGTCTTCAAACTGCAACAGCACGTCTGGCCAGCGTTGTTTCACAGCCTGGATAAATTCATCAACGAATTCATAGTATTCGTCGTCAGTGATACGCGGATTACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGAT  >  NC_000913/1554906‑1555174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A