Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,794	A→G	G449G (GGT→GGC)	dosC ←	diguanylate cyclase DosC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,794	0	A	G	95.6%	144.6 / ‑1.8	45	G449G (GGT→GGC)	dosC	diguanylate cyclase DosC
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (24/19); total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

CACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGTTT  >  NC_000913/1565661‑1565928

cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGt                                                                                                                                  >  2:692199/1‑140 (MQ=255)

cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGt                                                                                                                                  >  1:814448/1‑140 (MQ=255)

cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGt                                                                                                                                  >  1:884572/1‑140 (MQ=255)

    catcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCttt                                                                                                                              <  1:730505/140‑1 (MQ=255)

     atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCtttt                                                                                                                             >  2:490287/1‑140 (MQ=255)

     atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCtttt                                                                                                                             >  1:457877/1‑140 (MQ=255)

      tcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTg                                                                                                                            <  1:527556/140‑1 (MQ=255)

                cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACa                                                                                                                  >  1:715664/1‑140 (MQ=255)

                  cAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACaga                                                                                                                >  1:397117/1‑140 (MQ=255)

                        cGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCa                                                                                                          >  2:349931/1‑140 (MQ=255)

                                 aaaTGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTAtt                                                                                                 <  2:30008/140‑1 (MQ=255)

                                         tCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAg                                                                                         <  2:763139/140‑1 (MQ=255)

                                          cggcggCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                                                        <  2:814448/140‑1 (MQ=255)

                                          cggcggCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                                                        <  1:720256/140‑1 (MQ=255)

                                          cggcggCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                                                        <  1:349931/140‑1 (MQ=255)

                                                     tCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTc                                                                             >  2:755644/1‑140 (MQ=255)

                                                       cGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAg                                                                           >  2:631023/1‑140 (MQ=255)

                                                         cATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGa                                                                         >  2:403228/1‑140 (MQ=255)

                                                          aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                                                        <  1:490287/140‑1 (MQ=255)

                                                            cGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGa                                                                      <  1:123965/140‑1 (MQ=255)

                                                            cGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCACCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGa                                                                      <  1:185464/140‑1 (MQ=255)

                                                               ttAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCa                                                                   >  2:88269/1‑140 (MQ=255)

                                                                 aGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCAtt                                                                 <  2:457877/140‑1 (MQ=255)

                                                                            tACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATggcgg                                                      <  1:755644/140‑1 (MQ=255)

                                                                             aCCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATggcggc                                                     >  1:731458/1‑140 (MQ=255)

                                                                              ccTCTGCATCCTGGCGCATCTATAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCa                                                    <  1:631023/140‑1 (MQ=255)

                                                                                  tGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGa                                                >  1:607269/1‑140 (MQ=255)

                                                                                  tGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGa                                                <  2:397117/140‑1 (MQ=255)

                                                                                    cATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATg                                              >  2:257771/1‑140 (MQ=255)

                                                                                             gcATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag                                     <  2:884572/140‑1 (MQ=255)

                                                                                             gcATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag                                     <  1:257771/140‑1 (MQ=255)

                                                                                              cATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgaga                                    >  1:206735/1‑140 (MQ=255)

                                                                                                 cTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag                                     >  2:874713/1‑136 (MQ=255)

                                                                                                 cTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag                                     <  1:874713/136‑1 (MQ=255)

                                                                                                 cTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag                                     >  2:229955/1‑136 (MQ=255)

                                                                                                 cTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag                                     <  1:229955/136‑1 (MQ=255)

                                                                                                    aaGACTGGCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCaa                              >  1:340992/1‑140 (MQ=255)

                                                                                                         tGGCTTTCCGGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCt                         >  1:303489/1‑140 (MQ=255)

                                                                                                           gCTTTCCAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTc                       >  1:585236/1‑140 (MQ=255)

                                                                                                                ccAGAGTTCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGt                  <  2:715664/140‑1 (MQ=255)

                                                                                                                      ttCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCgg            >  2:426638/1‑140 (MQ=255)

                                                                                                                      ttCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCgg            >  2:767958/1‑140 (MQ=255)

                                                                                                                      ttCAACACGGTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCgg            >  2:884914/1‑140 (MQ=255)

                                                                                                                               gTTTCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGtt   <  1:403228/140‑1 (MQ=255)

                                                                                                                                tttCTGCCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGttt  >  2:736765/1‑140 (MQ=255)

CACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGAGGGCAATATCTTCGCCGTTTGCGGCTTTCAGTTT  >  NC_000913/1565661‑1565928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A