Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,568,560	T→C	Q88Q (CAA→CAG)	yddW ←	putative lipoprotein YddW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,568,560	0	T	C	100.0%	168.3 / NA	49	Q88Q (CAA→CAG)	yddW	putative lipoprotein YddW
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/21); total (28/21)

ATCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCGGTTGTGACGATTGTTGCGTCGTGGCTGGT  >  NC_000913/1568426‑1568689

aTCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCtgctgttg                                                                                                                              >  1:839843/1‑140 (MQ=255)

    tACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTaca                                                                                                                          <  2:839843/140‑1 (MQ=255)

     aCCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTacac                                                                                                                         >  1:311140/1‑140 (MQ=255)

        ggTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACggg                                                                                                                      >  2:168260/1‑140 (MQ=255)

           cATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGccc                                                                                                                   >  2:379341/1‑140 (MQ=255)

             tAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCgg                                                                                                                 <  1:173157/140‑1 (MQ=255)

                  tCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCtggt                                                                                                            <  2:752392/140‑1 (MQ=255)

                  tCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCtggt                                                                                                            <  1:268039/140‑1 (MQ=255)

                   cGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCtggtg                                                                                                           >  2:717020/1‑140 (MQ=255)

                    ggACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCtggtgg                                                                                                          >  2:849379/1‑140 (MQ=255)

                    ggACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCtggtgg                                                                                                          >  2:266426/1‑140 (MQ=255)

                    ggACCACGGCAAAATTTTCGATGGCCACAGGGCGATACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCtggtgg                                                                                                          >  1:304892/1‑140 (MQ=255)

                     gACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTggg                                                                                                         <  1:379341/140‑1 (MQ=255)

                     gACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTggg                                                                                                         >  2:361641/1‑140 (MQ=255)

                         aCGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTa                                                                                                     <  1:168260/140‑1 (MQ=255)

                         aCGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTa                                                                                                     <  2:388844/140‑1 (MQ=255)

                           ggCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATGGCCTGCTGTTGTACACGGGCCCGGCTGGTggg                                                                                                         >  2:740243/1‑134 (MQ=255)

                           ggCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTggg                                                                                                         >  2:285733/1‑134 (MQ=255)

                           ggCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTggg                                                                                                         <  1:740243/134‑1 (MQ=255)

                           ggCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTggg                                                                                                         <  1:285733/134‑1 (MQ=255)

                                          ggCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGa                                                                                    <  1:849379/140‑1 (MQ=255)

                                             cacaGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAAc                                                                                 <  2:592884/140‑1 (MQ=255)

                                                  ggCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTg                                                                            >  2:55798/1‑140 (MQ=255)

                                                            tCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAg                                                                  >  2:625019/1‑140 (MQ=255)

                                                            tCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAg                                                                  >  2:671079/1‑140 (MQ=255)

                                                            tCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAg                                                                  >  1:148997/1‑140 (MQ=255)

                                                            tCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAg                                                                  >  2:159963/1‑140 (MQ=255)

                                                            tCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAg                                                                  >  1:230860/1‑140 (MQ=255)

                                                                gCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCga                                                              <  1:587928/140‑1 (MQ=255)

                                                                 cTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCgag                                                             >  2:88094/1‑140 (MQ=255)

                                                                 cTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCgag                                                             <  1:55798/140‑1 (MQ=255)

                                                                          gAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTgg                                                    >  2:181564/1‑140 (MQ=255)

                                                                                 aCCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGTCAGTCGAGCCGAGAAACCGTGgccagcca                                             <  2:862185/140‑1 (MQ=255)

                                                                                   cGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGTGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGa                                           >  2:483177/1‑140 (MQ=255)

                                                                                    gtgtTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGAt                                          >  2:292925/1‑140 (MQ=255)

                                                                                     tgtTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATg                                         >  1:396142/1‑140 (MQ=255)

                                                                                        ttATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCa                                      <  2:448579/140‑1 (MQ=255)

                                                                                        ttATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCa                                      <  1:266426/140‑1 (MQ=255)

                                                                                           tGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGc                                   <  1:671079/140‑1 (MQ=255)

                                                                                               gagaCGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCg                               >  2:288917/1‑140 (MQ=255)

                                                                                                agaCGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCgg                              >  2:483729/1‑140 (MQ=255)

                                                                                                agaCGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCgg                              >  2:411576/1‑140 (MQ=255)

                                                                                                  aCGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCGGtt                            <  2:396142/140‑1 (MQ=255)

                                                                                                  aCGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCGGtt                            <  1:181564/140‑1 (MQ=255)

                                                                                                      tGCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCGGTTGTGa                        >  2:89485/1‑140 (MQ=255)

                                                                                                       gCAGATGATCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCGGTTGTGAc                       <  2:65893/140‑1 (MQ=255)

                                                                                                              atCCAGTTTGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCGGATGTGACGAttgtt                >  1:133223/1‑140 (MQ=255)

                                                                                                                     ttGTCGATCATCGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCGGTTGTGACGATTGTTGcgtcgt         >  1:857159/1‑140 (MQ=255)

                                                                                                                           atcatcGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCGGTTGTGACGATTGTTGCGTCGTGGCtgg   <  1:361641/140‑1 (MQ=255)

                                                                                                                            tcatcGCCTGCTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCGGTTGTGACGATTGTTGCGTCGTGGCtggt  >  2:350358/1‑140 (MQ=255)

ATCTTACCGGTCATAAGATCGGACCACGGCAAAATTTTCGATGGCCACAGGGCGGTACCGTCCGGCTTGACCTGGAAAAAGACCGTGTTTATGCCGAGACGTTGCAGATGATCCAGTTTGTCGATCATCGCCTGTTGTTGTACACGGGCCCGGCTGGTGGGGTTACTAATGTTAACCGAGGAAACCGGTGGCCAGTCGAGCCGAGAAACCGTGGCCAGCCAGATGCCACGCATCGGTTGTGACGATTGTTGCGTCGTGGCTGGT  >  NC_000913/1568426‑1568689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A