Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,570,741	T→C	E435E (GAA→GAG)	gadB ←	glutamate decarboxylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,570,741	0	T	C	96.0%	124.3 / ‑2.5	50	E435E (GAA→GAG)	gadB	glutamate decarboxylase B
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (29/19); total (30/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTG  >  NC_000913/1570611‑1570876

gTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAg                                                                                                                                <  1:2135817/140‑1 (MQ=255)

gTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAg                                                                                                                                <  1:2302288/140‑1 (MQ=255)

       tCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCAttt                                                                                                                         >  1:1701592/1‑140 (MQ=255)

        cGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCATTACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTc                                                                                                                        >  1:308463/1‑140 (MQ=255)

          ggACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGa                                                                                                                      >  1:2005320/1‑140 (MQ=255)

               ccgttaccgttaAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCcg                                                                                                                 <  2:1954729/140‑1 (MQ=255)

                cgttaccgttaAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCcgc                                                                                                                >  1:1077140/1‑140 (MQ=255)

                 gttaccgttaAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCcgcg                                                                                                               <  2:2004711/140‑1 (MQ=255)

                   taccgttaAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGcgac                                                                                                             <  2:501079/140‑1 (MQ=255)

                     ccgttaAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGcgacga                                                                                                           >  2:32233/1‑140 (MQ=255)

                     ccgttaAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGcgacga                                                                                                           >  1:2323653/1‑140 (MQ=255)

                       gttaAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGaca                                                                                                         >  2:1841067/1‑140 (MQ=255)

                         taAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGAcaca                                                                                                       <  2:1194622/140‑1 (MQ=255)

                         taAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGAcaca                                                                                                       >  2:582664/1‑140 (MQ=255)

                          aaaCGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACAt                                                                                                      >  2:228576/1‑140 (MQ=255)

                          aaaCGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACAt                                                                                                      >  2:1998066/1‑140 (MQ=255)

                              gTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATg                                                                                                  <  1:1693753/140‑1 (MQ=21)

                                  tCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGcgac                                                                                                             >  1:472638/1‑125 (MQ=25)

                                  tCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGcgac                                                                                                             <  2:472638/125‑1 (MQ=25)

                                   cAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCAt                                                                                             <  2:1701592/140‑1 (MQ=21)

                                    aGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATc                                                                                            <  1:32233/140‑1 (MQ=21)

                                          gTTTAGAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACg                                                                                      <  1:2358843/140‑1 (MQ=17)

                                          gTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACg                                                                                      >  2:1340948/1‑140 (MQ=18)

                                              aaaGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGt                                                                                  >  1:2333525/1‑140 (MQ=17)

                                              aaaGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGt                                                                                  >  2:1265339/1‑140 (MQ=17)

                                                aGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCg                                                                                >  1:2325514/1‑140 (MQ=33)

                                                aGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCg                                                                                >  2:997991/1‑140 (MQ=18)

                                                aGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCg                                                                                >  1:141010/1‑140 (MQ=18)

                                                aGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCg                                                                                <  2:452016/140‑1 (MQ=17)

                                                     ttctgttGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGc                                                                           >  1:342398/1‑140 (MQ=18)

                                                     ttctgttGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGc                                                                           >  2:1889841/1‑140 (MQ=17)

                                                     ttctgttGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGc                                                                           >  1:409289/1‑140 (MQ=18)

                                                     ttctgttGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGc                                                                           >  1:1525748/1‑140 (MQ=17)

                                                     ttctgttGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGc                                                                           >  1:590975/1‑140 (MQ=18)

                                                     ttctgttGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGc                                                                           >  2:2005586/1‑140 (MQ=18)

                                                         gttGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCa                                                                       <  1:1841067/140‑1 (MQ=14)

                                                                                                                     cTTCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCa           >  2:198173/1‑140 (MQ=11)

                                                                                                                      ttCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCAt          >  2:1701330/1‑140 (MQ=14)

                                                                                                                      ttCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCAt          >  2:563716/1‑140 (MQ=14)

                                                                                                                      ttCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCAt          >  2:2358134/1‑140 (MQ=14)

                                                                                                                       tCCAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATa         <  1:198173/140‑1 (MQ=17)

                                                                                                                        ccAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATAc        <  1:714170/140‑1 (MQ=18)

                                                                                                                        ccAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATAc        <  2:1911098/140‑1 (MQ=18)

                                                                                                                        ccAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATAc        >  2:71436/1‑140 (MQ=17)

                                                                                                                        ccAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATAc        <  2:996326/140‑1 (MQ=17)

                                                                                                                         cAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCCCCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACa       <  2:1929913/140‑1 (MQ=18)

                                                                                                                         cAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCCCCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACa       <  2:186909/140‑1 (MQ=18)

                                                                                                                         cAGCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACa       >  1:896731/1‑140 (MQ=17)

                                                                                                                           gCAACAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAgg     >  1:1904668/1‑140 (MQ=17)

                                                                                                                             aaCAGCTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGgt   <  1:489225/140‑1 (MQ=18)

                                                                                                                              aCAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCCCCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGgtg  <  1:1012083/140‑1 (MQ=18)

GTTCGTTTCGGGACACCGTTACCGTTAAACGTTATCAGGTATGTTTAAAGCTGTTCTGTTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTG  >  NC_000913/1570611‑1570876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A