Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,570,826	G→A	P407L (CCG→CTG)	gadB ←	glutamate decarboxylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,570,826	0	G	A	100.0%	84.8 / NA	39	P407L (CCG→CTG)	gadB	glutamate decarboxylase B
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/21); total (18/21)

TTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTACAGATGAACTCATA  >  NC_000913/1570729‑1570959

ttCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCAt                                                                                             >  2:74724/1‑140 (MQ=14)

ttCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCAt                                                                                             >  2:282381/1‑140 (MQ=14)

ttCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCAt                                                                                             >  1:880647/1‑140 (MQ=14)

 tCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATa                                                                                            <  2:824002/140‑1 (MQ=17)

            ttCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATAc                                                                                           >  1:1165352/1‑130 (MQ=17)

            ttCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATAc                                                                                           <  2:1165352/130‑1 (MQ=18)

            ttCAGCAAAGTCCATTTCGAAGCCACGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATc                                                                                 >  1:841802/1‑140 (MQ=17)

                    aGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCt                                                                         >  1:847719/1‑140 (MQ=17)

                       ccATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCa                                                                      <  1:477138/140‑1 (MQ=18)

                         aTTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCAcc                                                                    <  1:1011213/140‑1 (MQ=18)

                         aTTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCAcc                                                                    <  1:578784/140‑1 (MQ=18)

                           ttCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCa                                                                      >  2:959404/1‑136 (MQ=17)

                           ttCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCa                                                                      <  1:959404/136‑1 (MQ=18)

                               aaGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCttt                                                              <  1:41803/140‑1 (MQ=18)

                                                  tGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAAccgcc                                           >  1:875562/1‑140 (MQ=17)

                                                  tGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAAc                                               >  2:579387/1‑136 (MQ=17)

                                                  tGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAAc                                               <  1:579387/136‑1 (MQ=17)

                                                       aTCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGAt                                      >  1:387241/1‑140 (MQ=17)

                                                       aTCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGAt                                      >  2:619042/1‑140 (MQ=17)

                                                                 tGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTcc                            >  2:797541/1‑140 (MQ=17)

                                                                 tGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTcc                            >  2:402391/1‑140 (MQ=17)

                                                                   tCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCgg                          <  2:1088375/140‑1 (MQ=17)

                                                                   tCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCgg                          <  1:619042/140‑1 (MQ=17)

                                                                   tCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCgg                          >  1:806890/1‑140 (MQ=17)

                                                                   tCGGTGGCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCgg                          >  2:636101/1‑140 (MQ=17)

                                                                        ggCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCGGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGAc                     <  1:778023/140‑1 (MQ=17)

                                                                        ggCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGAc                     <  2:841802/140‑1 (MQ=17)

                                                                        ggCTTCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGAc                     <  2:875562/140‑1 (MQ=17)

                                                                           ttCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCg                  <  2:440311/140‑1 (MQ=17)

                                                                           ttCACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCg                  <  2:66223/140‑1 (MQ=17)

                                                                             cACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTa                <  2:1107618/140‑1 (MQ=17)

                                                                             cACCGCCGAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTa                >  1:596733/1‑140 (MQ=17)

                                                                                   cgAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTACAGATg          <  1:74724/140‑1 (MQ=17)

                                                                                   cgAGAGTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTACAGATg          <  1:282381/140‑1 (MQ=17)

                                                                                     agagTGAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTACAGATGaa        >  1:1119528/1‑140 (MQ=17)

                                                                                          gAAGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTACAGATGAACTCAt   <  1:797541/140‑1 (MQ=17)

                                                                                           aaGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTACAGATGAACTCATa  <  2:1119528/140‑1 (MQ=17)

                                                                                            aGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCATCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTa                >  1:746526/1‑125 (MQ=17)

                                                                                            aGGCCAGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTa                <  2:746526/125‑1 (MQ=17)

TTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGCGCATCACCACGATGTCGGTGGCTTCACCGCCGAGAGTGAAGGCCGGAACCTGCCAGCCGCGCAGACGCAGACGTTCAGAGAGGTCATACAGGGTGTATCCCGGATCTTCACCATCTTTCAGTTTGAAGCAAACCGCCGGGATGCCTTCGTCCGGGCGACCCGTACAGATGAACTCATA  >  NC_000913/1570729‑1570959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A