Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,576,027	A→G	G531G (GGT→GGC)	yddB ←	putative TonB‑dependent receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,576,027	0	A	G	100.0%	96.3 / NA	28	G531G (GGT→GGC)	yddB	putative TonB‑dependent receptor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/8); total (20/8)

CATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATA  >  NC_000913/1575900‑1576159

cATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGAt                                                                                                                          <  2:225835/140‑1 (MQ=255)

cATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGAt                                                                                                                          >  1:764845/1‑140 (MQ=255)

        tCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCa                                                                                                                  >  2:444692/1‑140 (MQ=255)

         cACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAg                                                                                                                 >  2:366692/1‑140 (MQ=255)

         cACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAg                                                                                                                 <  2:131183/140‑1 (MQ=255)

         cACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAg                                                                                                                 >  1:696883/1‑140 (MQ=255)

                 aCATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCGGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCg                                                                                                         >  1:502078/1‑140 (MQ=255)

                     aGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGata                                                                                                     >  1:525842/1‑140 (MQ=255)

                      gTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGatat                                                                                                    >  2:891269/1‑140 (MQ=255)

                      gTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGatat                                                                                                    <  1:444692/140‑1 (MQ=255)

                      gTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGatat                                                                                                    <  2:340644/140‑1 (MQ=255)

                          gCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAACTCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACg                                                                                                <  1:779843/140‑1 (MQ=255)

                          gCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACg                                                                                                <  2:133280/140‑1 (MQ=255)

                          gCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACg                                                                                                <  2:128464/140‑1 (MQ=255)

                                            ttACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAat                                                                              >  1:609122/1‑140 (MQ=255)

                                              aCCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAatat                                                                            >  1:132478/1‑140 (MQ=255)

                                              aCCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAatat                                                                            >  2:381874/1‑140 (MQ=255)

                                                    tttCTGCTGCAATCCCATTGCCGTTTCATCTTTATAAGGCT‑TTTTAAAATCCTAATACCGCGTCAGATTTTTATTGCCTGATCCCGTTTCCGTCCAGTTATTGCGGATATCACGTAATCCCATAAAAAAAATATTCCCGc                                                                      <  2:502078/140‑1 (MQ=255)

                                                                      tgcattttCATCGTTATAGGGTT‑TTTTAAAATCTTAATAACGCGTCAGTTTTTTATTGCTTAATCCCGATCCCGCCCAGTTATGGCGGATATCACTTACCCCAAAATAAAAAATTTTCCCGCCATAGTAACGGTTATAAc                                                    <  2:764845/134‑1 (MQ=255)

                                                                      tacaatttCATCTTTA‑AAGGTGTTTTTAAAACCCTGATAACGAGTAAGATTTTTATTCCGTTATACCGATCCCCTCCGGTTATTGCGGATCTCCCAAACCCCCATACAAAAAATATTCCCGCCATAGTAACGGTTATAAc                                                    <  2:525842/134‑1 (MQ=255)

                                                                          aGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACctgc                                                >  2:562608/1‑140 (MQ=255)

                                                                               aTCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTaa                                           >  1:515111/1‑140 (MQ=255)

                                                                                    tataAGGTT‑TTTTAAAATCCTGATACCGCTTCAGATTTTTATTGCCTGATCCCGATTCCGTCCAGCTATTGGGAATATCACATAATCCAATATCAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCAtt                                      <  2:315085/140‑1 (MQ=255)

                                                                                      taAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGa                                    >  1:731127/1‑140 (MQ=255)

                                                                                       agggCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAg                                   >  1:439175/3‑140 (MQ=255)

                                                                                              ttttCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGa                             >  1:40581/1‑140 (MQ=255)

                                                                                              ttttCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCCCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGa                             <  2:399053/140‑1 (MQ=255)

                                                                                               tttCAAATCCTGATAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGAt                            >  2:153422/1‑140 (MQ=255)

                                                                                                           aTAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAatat                >  1:518147/1‑140 (MQ=255)

                                                                                                           aTAACGCGTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAatat                >  1:745550/1‑140 (MQ=255)

                                                                                                                  gTCAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTc         >  2:552765/1‑140 (MQ=255)

                                                                                                                    cAGAGTTTTATTGCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCg       >  1:570564/1‑140 (MQ=255)

                                                                                                                         ttttATTGCCTGATCCCGTTTCCGCCCAGCTATTGCGGATATCACGTAATCCAATAAAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATa  <  2:40581/140‑1 (MQ=255)

                                                                                                                         ttttATTCCCTAATACCGTTTCCGTCCAGTTATTGCGGATATCACATAACCCAATAAAAAAAATTTTCCCGCCATAGTAACGGTTACAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATa  <  2:45798/140‑1 (MQ=255)

CATGCGCTTCACGGTAAACATAGTTTGCACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCG‑TTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATA  >  NC_000913/1575900‑1576159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A