Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,671,651	A→G	Q92Q (CAA→CAG)	asr →	acid shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,671,651	0	A	G	100.0%	135.4 / NA	39	Q92Q (CAA→CAG)	asr	acid shock protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/19); total (20/19)

CATAAAGCAGCACCTGCCCAGAAAGCGCAGGCGGCTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATG  >  NC_000913/1671520‑1671779

cATAAAGCAGCACCTGCCCAGAAAGCGCAGGCGGCTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCaaa                                                                                                                          <  2:318990/140‑1 (MQ=255)

   aaaGCAGCACCTGCCCAGAAAGCGCAGGCGGCTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAAcc                                                                                                                       <  1:583347/140‑1 (MQ=255)

     agcagcACCTGCCCAGAAAGCGCAGGCGGCTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACctg                                                                                                                     <  1:319841/140‑1 (MQ=255)

          caCCTGCCCAGAAAGCGCAGGCGGCTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGca                                                                                                                >  2:536460/1‑140 (MQ=255)

               gCCCAGAAAGCGCAGGCGGCTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAAcc                                                                                                           >  1:179898/1‑140 (MQ=255)

                          gcAGGCGGCTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAg                                                                                                >  2:300769/1‑140 (MQ=255)

                           cAGGCGGCTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACACGCAGCGTAAGt                                                                                               >  1:871864/1‑140 (MQ=255)

                               cggcTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTc                                                                                           <  2:741861/140‑1 (MQ=255)

                               cggcTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTc                                                                                           >  2:463461/1‑140 (MQ=255)

                                  cTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAAc                                                                                        <  1:79211/140‑1 (MQ=255)

                                    aaaaaGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAg                                                                                      <  1:15465/140‑1 (MQ=255)

                                        aGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAAt                                                                                  >  1:285886/1‑140 (MQ=255)

                                               tAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGgcg                                                                           >  1:361655/1‑140 (MQ=255)

                                                            gCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCgcgc                                                              >  2:663144/1‑140 (MQ=255)

                                                                     aaaGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAAtt                                                     >  2:436846/1‑140 (MQ=255)

                                                                      aaGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTa                                                    <  2:361655/140‑1 (MQ=255)

                                                                         ccccTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACgg                                                 <  1:300769/140‑1 (MQ=255)

                                                                               aaCAGAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTaa                                           <  2:682285/140‑1 (MQ=255)

                                                                               aaCAAAACGCGCAGCCAGCGGAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTaa                                           >  2:873711/1‑140 (MQ=255)

                                                                                         gcaggcagCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACg                                 >  2:880144/1‑140 (MQ=255)

                                                                                         gcaggcagCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACg                                 >  2:135088/1‑140 (MQ=255)

                                                                                         gcaggcagCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACg                                 >  1:506467/1‑140 (MQ=255)

                                                                                          caggcagCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGt                                <  1:436846/140‑1 (MQ=255)

                                                                                                gCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCa                          >  1:93428/1‑140 (MQ=255)

                                                                                                gCGAAGAAACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCa                          >  1:356811/1‑140 (MQ=255)

                                                                                                  gaagaaACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCAcc                        <  1:663144/140‑1 (MQ=255)

                                                                                                  gaagaaACACGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCAcc                        >  1:127925/1‑140 (MQ=255)

                                                                                                           cGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGc               >  1:646516/1‑140 (MQ=255)

                                                                                                           cGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGc               >  1:45124/1‑140 (MQ=255)

                                                                                                           cGCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGc               >  1:235898/1‑140 (MQ=255)

                                                                                                            gCCAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCg              <  1:880144/140‑1 (MQ=255)

                                                                                                             ccAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCgg             <  2:646516/140‑1 (MQ=255)

                                                                                                              cAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGa            <  2:45124/140‑1 (MQ=255)

                                                                                                              cAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGa            <  2:506467/140‑1 (MQ=255)

                                                                                                              cAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGa            <  1:463461/140‑1 (MQ=255)

                                                                                                              cAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGa            <  2:179898/140‑1 (MQ=255)

                                                                                                              cAAGAAACACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGa            <  2:127925/140‑1 (MQ=255)

                                                                                                                   aaCACAGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGgta       <  2:356811/140‑1 (MQ=255)

                                                                                                                        aGCCATCAGCAGCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTAtg  >  1:526206/1‑140 (MQ=255)

CATAAAGCAGCACCTGCCCAGAAAGCGCAGGCGGCTAAAAAGCATCATAAAAATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATG  >  NC_000913/1671520‑1671779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A