Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,063	G→A	T191T (ACC→ACT)	mepM ←	peptidoglycan DD‑endopeptidase MepM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,063	0	G	A	100.0%	142.7 / NA	41	T191T (ACC→ACT)	mepM	peptidoglycan DD‑endopeptidase MepM
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/22); total (19/22)

ACATTAACACCGCAAATTCATCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACGGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGC  >  NC_000913/1940927‑1941196

aCATTAACACCGCAAATTCATCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTa                                                                                                                                    <  2:103941/140‑1 (MQ=255)

  aTTAACACCGCAAATTCATCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTAcc                                                                                                                                  <  1:326751/140‑1 (MQ=255)

          cGCAAATTCATCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTcagca                                                                                                                          >  2:810034/1‑140 (MQ=255)

           gCAAATTCATCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTcagcag                                                                                                                         <  1:544767/140‑1 (MQ=255)

            cAAATTCATCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGa                                                                                                                        >  2:315/1‑140 (MQ=255)

             aaaTTCATCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGAt                                                                                                                       <  1:251751/140‑1 (MQ=255)

                 tCATCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGtt                                                                                                                   <  1:730372/140‑1 (MQ=255)

                  cATCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTa                                                                                                                  >  2:301760/1‑140 (MQ=255)

                    tCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAAc                                                                                                                >  2:772772/1‑140 (MQ=255)

                     cGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAAcc                                                                                                               <  1:846217/140‑1 (MQ=255)

                      gCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAAccc                                                                                                              <  1:315/140‑1 (MQ=255)

                       ccTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCa                                                                                                             <  1:260628/140‑1 (MQ=255)

                                     gCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCa                                                                                               <  1:172384/140‑1 (MQ=255)

                                      cGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCAt                                                                                              >  2:148015/1‑140 (MQ=255)

                                                   gCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCAtt                                                                                 >  2:690625/1‑140 (MQ=255)

                                                      aCTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTGCGCTGGTCATTGTa                                                                              >  2:548421/1‑140 (MQ=255)

                                                       cTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTaa                                                                             >  1:336730/1‑140 (MQ=255)

                                                       cTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTaa                                                                             <  1:148015/140‑1 (MQ=255)

                                                        tGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTaaa                                                                            >  1:308540/1‑140 (MQ=255)

                                                            tGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCg                                                                        <  1:810034/140‑1 (MQ=255)

                                                               cTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTa                                                                     >  1:278022/1‑140 (MQ=255)

                                                                tttAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAg                                                                    <  2:742006/140‑1 (MQ=255)

                                                                 ttAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGc                                                                   <  1:772772/140‑1 (MQ=255)

                                                                    aTCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGc                                                                >  2:275707/1‑140 (MQ=255)

                                                                     tCACTGCGCTCACTTCGGCGCTGGTTAACCCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCa                                                               <  1:567915/140‑1 (MQ=255)

                                                                        cTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTa                                                            <  1:331585/140‑1 (MQ=255)

                                                                         tGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTAc                                                           >  1:75651/1‑140 (MQ=255)

                                                                         tGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTAc                                                           >  2:699873/1‑140 (MQ=255)

                                                                         tGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTAc                                                           <  1:275707/140‑1 (MQ=255)

                                                                         tGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTAc                                                           >  1:188593/1‑140 (MQ=255)

                                                                         tGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTAc                                                           >  1:110011/1‑140 (MQ=255)

                                                                             cTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTc                                                       >  1:806580/1‑140 (MQ=255)

                                                                                   tCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGt                                                 >  2:892842/1‑140 (MQ=255)

                                                                                     ggCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTc                                               >  1:132458/1‑140 (MQ=255)

                                                                                                     gCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACgaga                               <  2:724471/140‑1 (MQ=255)

                                                                                                       gTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGaca                             <  2:602194/140‑1 (MQ=255)

                                                                                                       gTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGaca                             <  1:349716/140‑1 (MQ=255)

                                                                                                       gTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGaca                             <  2:769145/140‑1 (MQ=255)

                                                                                                                    gCAACAAAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAg                >  1:209419/1‑140 (MQ=255)

                                                                                                                     caacaaAGCTTCCCCCGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAgg               <  2:209419/140‑1 (MQ=255)

                                                                                                                                  cccGACAGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGc  <  1:113393/140‑1 (MQ=255)

ACATTAACACCGCAAATTCATCGCCTTTTTTCAGTTTGCGGAAATCCATTTGCCACTGCATGGCTTTAATCACTGCGCTCACTTCGGCGCTGGTTAAACCGGCGTTTCTGGCGCTGGCAACAAAGCTTCCCCCGACGGTACCTTTCAGCAGATTGTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGC  >  NC_000913/1940927‑1941196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A