Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,755	T→C	T277A (ACG→GCG)	znuA ←	Zn(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,755	0	T	C	100.0%	140.0 / NA	40	T277A (ACG→GCG)	znuA	Zn(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/22); total (18/22)

CGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTCAACCAACTGTGTTCTTATTT  >  NC_000913/1941619‑1941865

cGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTcc                                                                                                             <  2:153676/140‑1 (MQ=255)

      aTCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACg                                                                                                       <  1:671205/140‑1 (MQ=255)

      aTCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACg                                                                                                       <  1:417794/140‑1 (MQ=255)

        cTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGaa                                                                                                     <  1:320030/140‑1 (MQ=255)

        cTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGaa                                                                                                     <  1:515948/140‑1 (MQ=255)

             gCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGAt                                                                                                >  1:412/1‑140 (MQ=255)

                 gTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTcc                                                                                            >  1:834070/1‑140 (MQ=255)

                   aaTCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGaa                                                                                                     <  2:641726/129‑1 (MQ=255)

                   aaTCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGaa                                                                                                     >  1:641726/1‑129 (MQ=255)

                     tCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCAt                                                                                                          <  2:621157/122‑1 (MQ=255)

                     tCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCAt                                                                                                          >  1:621157/1‑122 (MQ=255)

                         ttCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCg                                                                                    <  2:412/140‑1 (MQ=255)

                          tCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGa                                                                                   <  2:257010/140‑1 (MQ=255)

                          tCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGa                                                                                   <  2:179394/140‑1 (MQ=255)

                            cTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACg                                                                                 <  1:338615/140‑1 (MQ=255)

                            cTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACg                                                                                 <  1:236093/140‑1 (MQ=255)

                               aTTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTcc                                                                                            >  1:549603/1‑126 (MQ=255)

                               aTTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTcc                                                                                            <  2:549603/126‑1 (MQ=255)

                                       ccTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACg                                                                                 >  1:631180/1‑129 (MQ=255)

                                       ccTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACg                                                                                 <  2:631180/129‑1 (MQ=255)

                                         tttCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCg                                                                    <  2:834070/140‑1 (MQ=255)

                                         tttCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCg                                                                    <  2:130414/140‑1 (MQ=255)

                                                             ggTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGcc                                                              >  1:303128/1‑126 (MQ=255)

                                                             ggTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGcc                                                              <  2:303128/126‑1 (MQ=255)

                                                              gTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAg                                               >  2:870155/1‑140 (MQ=255)

                                                                      aTTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACg                                       >  1:183281/1‑140 (MQ=255)

                                                                                      tGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCt                       <  1:870155/140‑1 (MQ=255)

                                                                                        aaTAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAACAACGCAGGTTGCTTTTTGCTca                     >  1:500383/1‑140 (MQ=255)

                                                                                          tAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTcaac                   <  2:18915/140‑1 (MQ=255)

                                                                                          tAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTcaac                   >  2:763195/1‑140 (MQ=255)

                                                                                             cTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTcaaccaa                <  1:513956/140‑1 (MQ=255)

                                                                                             cTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTcaaccaa                <  1:198392/140‑1 (MQ=255)

                                                                                                     aCCCAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTCAACCAACTGTGTTc        >  1:815125/1‑140 (MQ=255)

                                                                                                      cccAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTcaac                   >  2:316359/1‑128 (MQ=255)

                                                                                                      cccAGTTTGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTcaac                   <  1:316359/128‑1 (MQ=255)

                                                                                                       ccAGTTTGATATTCGTCACCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTCAACCAACTGTGTTCtt      >  2:601759/1‑140 (MQ=255)

                                                                                                           tttGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTCAACCAACTGTGTTCTTAttt  >  2:224101/1‑140 (MQ=255)

                                                                                                           tttGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTCAACCAACTGTGTTCTTAttt  >  2:12905/1‑140 (MQ=255)

                                                                                                           tttGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTCAACCAACTGTGTTCTTAttt  >  1:653169/1‑140 (MQ=255)

                                                                                                           tttGATATTCGTCCCCAGGGGATCCAACGCTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTCAACCAACTGTGTTCTTAttt  >  2:756461/1‑140 (MQ=255)

CGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGCAGGTTGCTTTTTGCTCAACCAACTGTGTTCTTATTT  >  NC_000913/1941619‑1941865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A