Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,149,460	T→C	K509R (AAG→AGG)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,149,460	0	T	C	100.0%	107.0 / NA	31	K509R (AAG→AGG)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/13); total (18/13)

TTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCTTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACC  >  NC_000913/2149325‑2149591

ttCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAActtctgcctctg                                                                                                                                 >  2:791117/1‑140 (MQ=255)

      tGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTc                                                                                                                           >  2:802361/1‑140 (MQ=255)

             cGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCa                                                                                                                    <  2:417345/140‑1 (MQ=255)

                        gTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAg                                                                                                         >  1:737327/1‑140 (MQ=255)

                                tGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCa                                                                                                 <  1:802361/140‑1 (MQ=255)

                                 gCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCag                                                                                                >  1:218565/1‑140 (MQ=255)

                                     cTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAgcg                                                                                            <  1:558235/140‑1 (MQ=255)

                                             aTACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTtctc                                                                                    >  1:677840/1‑140 (MQ=255)

                                                ccAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCtcttc                                                                                 <  1:620381/140‑1 (MQ=255)

                                                  aaaaGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTATGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTtct                                                                                     <  1:822492/134‑1 (MQ=255)

                                                  aaaaGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTATGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTtct                                                                                     >  2:822492/1‑134 (MQ=255)

                                                   aaaGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATAAAGTGCTGCCAGAGCGCGTTTCTCttcttc                                                                              >  1:621255/1‑140 (MQ=255)

                                                       aCCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGt                                                                          <  1:403102/140‑1 (MQ=255)

                                                          gTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAAc                                                                       >  1:560913/1‑140 (MQ=255)

                                                                 cgcTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGaa                                                                >  1:224011/1‑140 (MQ=255)

                                                                          cgcAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCt                                                       <  1:547677/140‑1 (MQ=255)

                                                                                     cGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCg                                            <  2:560913/140‑1 (MQ=255)

                                                                                         gggAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCa                                        >  1:198827/1‑140 (MQ=255)

                                                                                          ggAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCAt                                       >  1:552236/1‑140 (MQ=255)

                                                                                             aTAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGt                                    >  1:341647/1‑140 (MQ=255)

                                                                                                 agGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGt                                <  1:64645/140‑1 (MQ=255)

                                                                                                  ggCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAA‑TTCGTCCTTCATTTTTTCGAGCATCGTCCGttt                              <  2:224011/140‑1 (MQ=255)

                                                                                                    cAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCAtttttt                                              >  1:609001/1‑123 (MQ=255)

                                                                                                    cAACATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCAtttttt                                              <  2:609001/123‑1 (MQ=255)

                                                                                                      aCATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTg                             >  1:638841/1‑138 (MQ=255)

                                                                                                      aCATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTg                             <  2:638841/138‑1 (MQ=255)

                                                                                                      aCATCAATAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGc                           >  1:664069/1‑140 (MQ=255)

                                                                                                          cAATAAAAAATCCCTCCAGAAACTTCTACCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATa                       >  1:490661/1‑140 (MQ=255)

                                                                                                            aTAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATaaa                     <  2:369460/140‑1 (MQ=255)

                                                                                                             tAAAAAATCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTTCGTTTGGCGATaaaa                    >  2:882620/1‑140 (MQ=255)

                                                                                                                    tCCCTCCAGAAACTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCa             >  1:492907/1‑140 (MQ=255)

                                                                                                                               aCTTCTGCCTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTAcc  >  1:819765/1‑140 (MQ=255)

TTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATAAAAAATCCCTCCAGAAACTTCTGCTTCTGCCGTTCCCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACC  >  NC_000913/2149325‑2149591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A