Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 163 52.9 1786516 99.3% 1774010 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,212,866 A→G C33R (TGT→CGT)  btsR ← DNA‑binding transcriptional activator BtsR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,212,8660AG97.1% 229.4 / ‑2.7 69C33R (TGT→CGT) btsRDNA‑binding transcriptional activator BtsR
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (34/33);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTCGGGT  >  NC_000913/2212730‑2213000
                                                                                                                                        |                                                                                                                                      
aCAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGctc                                                                                                                                     >  2:23633/1‑140 (MQ=255)
    tataCGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCa                                                                                                                                 >  1:212992/1‑140 (MQ=255)
     ataCGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCaa                                                                                                                                <  2:736808/140‑1 (MQ=255)
      taCGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAAc                                                                                                                               <  1:185801/140‑1 (MQ=255)
         gggCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGAt                                                                                                                            >  1:18860/1‑140 (MQ=255)
         gggCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGAt                                                                                                                            >  2:660423/1‑140 (MQ=255)
           gCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGAttt                                                                                                                          <  2:742130/140‑1 (MQ=255)
           gCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCAGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGAttt                                                                                                                          >  1:478764/1‑140 (MQ=255)
                gTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAata                                                                                                                     <  2:32712/140‑1 (MQ=255)
                 ttCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAatat                                                                                                                    >  2:127667/1‑140 (MQ=255)
                         cAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGc                                                                                                            >  2:376671/1‑140 (MQ=255)
                           aGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTc                                                                                                          >  2:58014/1‑140 (MQ=255)
                           aGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTc                                                                                                          >  1:196275/1‑140 (MQ=255)
                            gCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTcc                                                                                                         >  2:57115/1‑140 (MQ=255)
                                    accaTTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCaaaaa                                                                                                 >  1:279646/1‑140 (MQ=255)
                                    accaTTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCaaaaa                                                                                                 >  1:132743/1‑140 (MQ=255)
                                     ccaTTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAAt                                                                                                >  1:180568/1‑140 (MQ=255)
                                       aTTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATac                                                                                              >  1:32975/1‑140 (MQ=255)
                                        tttCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATaca                                                                                             >  2:408151/1‑140 (MQ=255)
                                         ttCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATacac                                                                                            <  1:660423/140‑1 (MQ=255)
                                              gACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAgg                                                                                       <  2:158978/140‑1 (MQ=255)
                                              gACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAgg                                                                                       <  1:478735/140‑1 (MQ=255)
                                              gACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAgg                                                                                       <  2:454297/140‑1 (MQ=255)
                                               aCCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGt                                                                                      >  2:841607/1‑140 (MQ=255)
                                                ccACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGtt                                                                                     >  1:596957/1‑140 (MQ=255)
                                                   cTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTtctc                                                                                  >  2:788430/1‑140 (MQ=255)
                                                   cTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGCTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTtctc                                                                                  >  1:614829/1‑140 (MQ=255)
                                                     gATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTccc                                                                                <  1:540350/140‑1 (MQ=255)
                                                            ggCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTaa                                                                         <  2:180568/140‑1 (MQ=255)
                                                              cATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACg                                                                       >  2:792521/1‑140 (MQ=255)
                                                                   ggATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTtca                                                                  <  1:376671/140‑1 (MQ=255)
                                                                         cGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCg                                                            <  1:58321/140‑1 (MQ=255)
                                                                         cGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCg                                                            <  1:329387/140‑1 (MQ=255)
                                                                           agaAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGAc                                                          >  1:584059/1‑140 (MQ=255)
                                                                             aaaCAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACaa                                                        >  2:123018/1‑140 (MQ=255)
                                                                                      aTCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACtt                                               >  2:204555/1‑140 (MQ=255)
                                                                                           ggCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACtt                                               >  1:444187/1‑135 (MQ=255)
                                                                                           ggCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACtt                                               <  2:444187/135‑1 (MQ=255)
                                                                                           ggCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATc                                          >  2:490940/1‑140 (MQ=255)
                                                                                            gcgcAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCa                                         <  2:212992/140‑1 (MQ=255)
                                                                                             cgcAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCAt                                        >  2:285184/1‑140 (MQ=255)
                                                                                                 gTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCt                                    <  1:123018/140‑1 (MQ=255)
                                                                                                 gTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCt                                    <  1:57115/140‑1 (MQ=255)
                                                                                                   ttATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCg                                  <  2:52280/140‑1 (MQ=255)
                                                                                                   ttATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCg                                  <  1:127667/140‑1 (MQ=255)
                                                                                                    tATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGt                                 <  1:408151/140‑1 (MQ=255)
                                                                                                    tATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGt                                 <  2:32975/140‑1 (MQ=255)
                                                                                                    tATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGt                                 <  1:204555/140‑1 (MQ=255)
                                                                                                    tATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGt                                 <  1:841607/140‑1 (MQ=255)
                                                                                                         aCCGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTc                            <  1:285184/140‑1 (MQ=255)
                                                                                                          ccGCGCCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTcc                           >  1:231057/1‑140 (MQ=255)
                                                                                                            gcgcCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACATTTAAGACTTTAATCATGCCTCGTCCCTCCAt                         >  1:694412/1‑140 (MQ=255)
                                                                                                            gcgcCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCAt                         >  2:484717/1‑140 (MQ=255)
                                                                                                            gcgcCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCAt                         >  1:560138/1‑140 (MQ=255)
                                                                                                            gcgcCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACACAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCAt                         >  2:257762/1‑140 (MQ=255)
                                                                                                             cgcCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGcc                                     <  2:309567/127‑1 (MQ=255)
                                                                                                             cgcCGATCCCTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGcc                                     >  1:309567/1‑127 (MQ=255)
                                                                                                                    cccTTCCACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCg                 <  2:29785/140‑1 (MQ=255)
                                                                                                                          cACGGCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGt           >  1:629219/1‑140 (MQ=255)
                                                                                                                             ggCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTAt        <  1:788430/140‑1 (MQ=255)
                                                                                                                              gCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACATTTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTAtt       <  2:694412/140‑1 (MQ=255)
                                                                                                                              gCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTAtt       <  1:884423/140‑1 (MQ=255)
                                                                                                                              gCGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTAtt       <  2:18860/140‑1 (MQ=255)
                                                                                                                               cGTTTGAACGCTCTCCAACGATTTCAATGTCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTc      <  2:478764/140‑1 (MQ=255)
                                                                                                                               cGTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTc      <  2:231057/140‑1 (MQ=255)
                                                                                                                                gTTTGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTCg     <  2:629219/140‑1 (MQ=255)
                                                                                                                                 tttGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTCgg    <  2:596957/140‑1 (MQ=255)
                                                                                                                                 tttGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTCgg    <  1:58014/140‑1 (MQ=255)
                                                                                                                                 tttGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTCgg    >  2:204325/1‑140 (MQ=255)
                                                                                                                                   tGAACGCTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTCGGgt  >  1:591717/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTCGGGT  >  NC_000913/2212730‑2213000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A