Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 258 142.3 4824480 99.1% 4781059 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,213,464 A→G V394A (GTG→GCG)  btsS ← high‑affinity pyruvate receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,213,4640AG100.0% 245.4 / NA 70V394A (GTG→GCG) btsShigh‑affinity pyruvate receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/31);  total (39/31)

TTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCA  >  NC_000913/2213331‑2213596
                                                                                                                                     |                                                                                                                                    
ttGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCtt                                                                                                                                <  2:31791/140‑1 (MQ=255)
ttGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCtt                                                                                                                                <  1:824754/140‑1 (MQ=255)
ttGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCtt                                                                                                                                <  1:2286737/140‑1 (MQ=255)
  gCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTa                                                                                                                              <  1:2381018/140‑1 (MQ=255)
  gCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTa                                                                                                                              <  1:1819060/140‑1 (MQ=255)
   cAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGCAGGCCGCTTCAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTaa                                                                                                                             <  2:2156644/140‑1 (MQ=255)
     gATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATg                                                                                                                           <  1:941698/140‑1 (MQ=255)
           cATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTa                                                                                                                     >  1:1776821/1‑140 (MQ=255)
            aTTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTaa                                                                                                                    <  1:10326/140‑1 (MQ=255)
             ttCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAg                                                                                                                   >  1:725387/1‑140 (MQ=255)
               cacaTGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAgcg                                                                                                                 >  2:751400/1‑140 (MQ=255)
               cacaTGCTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAgcg                                                                                                                 >  1:2144101/1‑140 (MQ=255)
                    gTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTa                                                                                                            >  1:1937081/1‑140 (MQ=255)
                    gTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTa                                                                                                            >  2:1671397/1‑140 (MQ=255)
                    gTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTa                                                                                                            >  2:1094192/1‑140 (MQ=255)
                     ttCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTaa                                                                                                                             <  2:784987/122‑1 (MQ=255)
                     ttCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTaa                                                                                                                             >  1:784987/1‑122 (MQ=255)
                     ttCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTaa                                                                                                           >  2:2015289/1‑140 (MQ=255)
                     ttCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTaa                                                                                                           >  1:2330371/1‑140 (MQ=255)
                            tcgtcgGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGTTACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACaaaaa                                                                                                    >  2:788299/1‑140 (MQ=255)
                            tcgtcgGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACaaaaa                                                                                                    >  2:1181854/1‑140 (MQ=255)
                            tcgtcgGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACaaaaa                                                                                                    >  1:606531/1‑140 (MQ=255)
                              gtcgGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAAt                                                                                                  <  1:751400/140‑1 (MQ=255)
                                  gCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATgggg                                                                                              <  1:1296561/140‑1 (MQ=255)
                                         tAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGAtt                                                                                           <  1:1365917/136‑1 (MQ=255)
                                         tAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGAtt                                                                                           >  2:1365917/1‑136 (MQ=255)
                                         tAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCAcc                                                                                       <  2:1211429/140‑1 (MQ=255)
                                          aaCAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCt                                                                                      <  1:1181854/140‑1 (MQ=255)
                                            cAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTgg                                                                                    <  2:1760636/140‑1 (MQ=255)
                                                cTCCGAAGGTCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGATCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATgg                                                                                                <  1:655393/124‑1 (MQ=255)
                                                cTCCGAAGGTCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGATCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATgg                                                                                                >  2:655393/1‑124 (MQ=255)
                                                cTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAATATACTGCACCAGCTGGCTGGCCTGTTCGCTGCCTCGGCGGATCTCCGCTTTAATGGTGTTAAGCGCATTAAAAAAAAAATGGGGGTTAACCAgggagg                                                                                >  2:707024/1‑137 (MQ=255)
                                                   cGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGaa                                                                             <  2:1138573/140‑1 (MQ=255)
                                                    gAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAg                                                                            <  2:1776821/140‑1 (MQ=255)
                                                     aaGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGc                                                                           <  1:1671397/140‑1 (MQ=255)
                                                     aaGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGc                                                                           <  2:725387/140‑1 (MQ=255)
                                                      aGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCa                                                                          >  2:812898/1‑140 (MQ=255)
                                                      aGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCa                                                                          >  2:1069655/1‑140 (MQ=255)
                                                          cGCTTTAAGTTTTTGCGTAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCTCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGttt                                                                      <  2:2144101/140‑1 (MQ=255)
                                                          cGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGttt                                                                      <  2:606531/140‑1 (MQ=255)
                                                          cGCTTTAAGCTTTTGCGGAAAAAAGTGGAAAGATACAGCACCAGCTGGCAGGCCTGTTCGCTGTCGCGCCGGAACGGCACCTTAATGGCGTTAAGCGCATTAAACAAAAAATGGGGGATCAAACGGGCGAGAAGCAGatt                                                                      >  2:1191213/1‑140 (MQ=255)
                                                           gCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTg                                                                     >  2:1035113/1‑140 (MQ=255)
                                                             tttAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGGGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGAt                                                                   >  1:635687/1‑140 (MQ=255)
                                                               tAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTGAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGAtct                                                                 <  1:1191213/140‑1 (MQ=255)
                                                                   tttttGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGa                                                             <  2:1630338/140‑1 (MQ=255)
                                                                      ttGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTg                                                          >  1:961247/1‑140 (MQ=255)
                                                                      ttGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTg                                                          >  1:2008433/1‑140 (MQ=255)
                                                                       tGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTgg                                                         >  2:1219279/1‑140 (MQ=255)
                                                                       tGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTgg                                                         >  2:675474/1‑140 (MQ=255)
                                                                           gAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGa                                                     <  2:961247/140‑1 (MQ=255)
                                                                              aaaaGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCa                                                  <  1:1069655/140‑1 (MQ=255)
                                                                                        aGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTg                                        <  2:2330371/140‑1 (MQ=255)
                                                                                           tACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCg                                     <  2:1937081/140‑1 (MQ=255)
                                                                                             cTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCt                                   >  2:742547/1‑140 (MQ=255)
                                                                                                 aCCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCTCCTGGGCGTGAAGCAGTTTGACCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCata                               <  2:635687/140‑1 (MQ=255)
                                                                                                 aCCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCata                               >  1:157854/1‑140 (MQ=255)
                                                                                                    aGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCata                               >  2:2206568/1‑137 (MQ=255)
                                                                                                    aGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCata                               <  1:2206568/137‑1 (MQ=255)
                                                                                                    aGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTg                            >  1:894029/1‑140 (MQ=255)
                                                                                                    aGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTg                            >  1:460047/1‑140 (MQ=255)
                                                                                                     gctggctgGTCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGc                           >  1:1605000/1‑140 (MQ=255)
                                                                                                     gctggctgGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGc                           >  2:13666/1‑140 (MQ=255)
                                                                                                     gctggctgGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGc                           >  2:845723/1‑140 (MQ=255)
                                                                                                     gctggctgGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGc                           >  1:186451/1‑140 (MQ=255)
                                                                                                     gctggctgGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGc                           <  1:681773/140‑1 (MQ=255)
                                                                                                     gctggctgGCCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGc                           >  2:2310258/1‑140 (MQ=255)
                                                                                                        ggctggcCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCg                        >  1:781032/1‑140 (MQ=255)
                                                                                                          ctggcCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGc                      >  2:432658/1‑140 (MQ=255)
                                                                                                             gcCTGTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAg                   >  2:414679/1‑140 (MQ=255)
                                                                                                                 gTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGcc                          <  2:736321/129‑1 (MQ=255)
                                                                                                                 gTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGcc                          >  1:736321/1‑129 (MQ=255)
                                                                                                                 gTTCGCTGTCGCGGCGGATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATc               >  1:2056102/1‑140 (MQ=255)
                                                                                                                              gcggATCGCCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCa  >  2:589801/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TTGCAGATAAGCATTCACATGTTCAATTTCGTCGGCGAGAGTAACAAACTCCGAAGGCCGCTTTAAGTTTTTGCGGAAAAAAGTGGAAAGATACTGCACCAGCTGGCTGGCCTGTTCGCTGTCGCGGCGGATCACCGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCA  >  NC_000913/2213331‑2213596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A