Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,296,418	T→C	T203A (ACC→GCC)	ccmB ←	heme trafficking system membrane protein CcmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,296,418	0	T	C	100.0%	128.7 / NA	38	T203A (ACC→GCC)	ccmB	heme trafficking system membrane protein CcmB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/24); total (14/24)

TGGTGGGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGTGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAAT‑GGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCAC  >  NC_000913/2296285‑2296550

tggtggGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGcgcctgc                                                                                                                                 >  1:101534/1‑140 (MQ=255)

 ggtggGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGcgcctgcc                                                                                                                                >  1:71174/1‑140 (MQ=255)

  gtggGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCa                                                                                                                               >  2:506401/1‑140 (MQ=255)

       aTCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAAc                                                                                                                          >  2:440734/1‑140 (MQ=255)

       aTCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAAc                                                                                                                          >  2:730921/1‑140 (MQ=255)

       aTCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAAc                                                                                                                          <  2:71174/140‑1 (MQ=255)

           ccAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACgcgc                                                                                                                      <  1:730921/140‑1 (MQ=255)

           ccAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACgcgc                                                                                                                      <  2:640746/140‑1 (MQ=255)

            cAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGcc                                                                                                                     <  1:440734/140‑1 (MQ=255)

             aGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCt                                                                                                                    <  1:591503/140‑1 (MQ=255)

                      aGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCGGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGCCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGcc                                                                                                           <  2:435159/140‑1 (MQ=255)

                      aGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGcc                                                                                                           <  1:519097/140‑1 (MQ=255)

                          ttttCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGAt                                                                                                       >  1:474824/1‑140 (MQ=255)

                               cacaTAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCg                                                                                                  >  1:875742/1‑140 (MQ=255)

                                  aTAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCa                                                                                               >  1:110474/1‑140 (MQ=255)

                                     gTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACg                                                                                            <  2:101534/140‑1 (MQ=255)

                                       ttCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACggg                                                                                          >  2:735985/1‑140 (MQ=255)

                                        tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                         <  1:177213/140‑1 (MQ=255)

                                        tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                         <  2:474824/140‑1 (MQ=255)

                                        tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                         <  1:567106/140‑1 (MQ=255)

                                         cGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGCCTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCa                                                                                        <  1:168927/140‑1 (MQ=255)

                                                aGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCAt                                                                                 >  2:15985/1‑140 (MQ=255)

                                                 gACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaa                                                                                       <  2:118222/133‑1 (MQ=255)

                                                 gACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaa                                                                                       >  1:118222/1‑133 (MQ=255)

                                                            aaaTCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCATCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTc                                                                     <  2:423603/140‑1 (MQ=255)

                                                                cAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATc                                                                 >  2:640211/1‑140 (MQ=255)

                                                                    aaTCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCgcgg                                                             >  2:166082/1‑140 (MQ=255)

                                                                            gTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCa                                                     <  2:875742/140‑1 (MQ=255)

                                                                                ttGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCGAAGa                                                 >  2:253748/1‑140 (MQ=255)

                                                                                 tGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGAt                                                <  2:647687/140‑1 (MQ=255)

                                                                                 tGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGAt                                                <  1:124956/140‑1 (MQ=255)

                                                                                    aTGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAg                                             <  2:110474/140‑1 (MQ=255)

                                                                                         gATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAAT‑g                                       <  1:166082/140‑1 (MQ=255)

                                                                                          aTTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAAT‑gg                                      >  2:31538/1‑140 (MQ=255)

                                                                                          aTTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAAT‑gg                                      >  2:253588/1‑140 (MQ=255)

                                                                                            tCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAAT‑GGGa                                    <  2:675064/140‑1 (MQ=255)

                                                                                            tCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAAT‑GGGa                                    <  1:735985/140‑1 (MQ=255)

                                                                                                         gccgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAAT‑GGGATAGTCAGCGGTaa                       <  2:524172/140‑1 (MQ=255)

                                                                                                                        cTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAAT‑GGGATAGTCAGCGGTAACACCAGTATGCTGag        <  2:354468/140‑1 (MQ=255)

                                                                                                                               tCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGcac  <  1:108291/140‑1 (MQ=255)

TGGTGGGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGTGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAAT‑GGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCAC  >  NC_000913/2296285‑2296550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A