Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,296,418	T→C	T203A (ACC→GCC)	ccmB ←	heme trafficking system membrane protein CcmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,296,418	0	T	C	100.0%	167.7 / NA	48	T203A (ACC→GCC)	ccmB	heme trafficking system membrane protein CcmB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/29); total (19/29)

GCCGTGGTGGGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGTGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCACACCA  >  NC_000913/2296281‑2296554

gCCGTGGTGGGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGgcgc                                                                                                                                        >  1:618132/1‑140 (MQ=255)

gCCGTGGTGGGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGgcgc                                                                                                                                        >  1:389058/1‑140 (MQ=255)

      gtggGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCa                                                                                                                                  >  2:344458/1‑140 (MQ=255)

           aTCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAAc                                                                                                                             <  2:389058/140‑1 (MQ=255)

           aTCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAAc                                                                                                                             >  2:40334/1‑140 (MQ=255)

              gCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAAcgcg                                                                                                                          >  2:433511/1‑140 (MQ=255)

              gCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAAcgcg                                                                                                                          >  2:326583/1‑140 (MQ=255)

                     gATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTaaaa                                                                                                                   <  2:618132/140‑1 (MQ=255)

                                       tAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCaa                                                                                                 <  1:147614/140‑1 (MQ=255)

                                        aGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAAc                                                                                                >  2:332252/1‑140 (MQ=255)

                                         gTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACg                                                                                               <  1:564032/140‑1 (MQ=255)

                                          tttCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACgg                                                                                              >  1:512143/1‑140 (MQ=255)

                                            tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                            <  1:40334/140‑1 (MQ=255)

                                            tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                            <  1:326583/140‑1 (MQ=255)

                                            tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                            <  1:799429/140‑1 (MQ=255)

                                            tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                            >  2:601243/1‑140 (MQ=255)

                                            tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                            <  1:37038/140‑1 (MQ=255)

                                            tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                            <  1:344458/140‑1 (MQ=255)

                                             cGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCa                                                                                           <  1:705549/140‑1 (MQ=255)

                                              gATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaa                                                                                          <  1:601243/140‑1 (MQ=255)

                                               aTACCAGACTCGAACAAAAATCAGTAATCCATCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaaa                                                                                         <  2:743242/140‑1 (MQ=255)

                                                  ccAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGc                                                                                      >  2:480050/1‑140 (MQ=255)

                                                           aaCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGcc                                                                             >  2:27424/1‑140 (MQ=255)

                                                                aaaTCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTc                                                                        <  1:480050/140‑1 (MQ=255)

                                                                            cAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGt                                                            >  2:402454/1‑140 (MQ=255)

                                                                               cGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGc                                                         >  2:410740/1‑140 (MQ=255)

                                                                                gTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCa                                                        <  1:332252/140‑1 (MQ=255)

                                                                                   aTTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAg                                                     <  1:798622/140‑1 (MQ=255)

                                                                                     tGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGAt                                                   <  2:435713/140‑1 (MQ=255)

                                                                                         tGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCgcggcg                                                              >  2:813068/1‑125 (MQ=255)

                                                                                         tGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCgcggcg                                                              <  1:813068/125‑1 (MQ=255)

                                                                                          gCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTa                                              <  1:639509/140‑1 (MQ=255)

                                                                                          gCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTa                                              <  1:27424/140‑1 (MQ=255)

                                                                                            tGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCTAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAAt                                            <  2:177665/140‑1 (MQ=255)

                                                                                              aTTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCa                                                        <  1:623559/126‑1 (MQ=255)

                                                                                              aTTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCa                                                        >  2:623559/1‑126 (MQ=255)

                                                                                              aTTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATg                                                  <  1:93916/132‑1 (MQ=255)

                                                                                              aTTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATg                                                  >  2:93916/1‑132 (MQ=255)

                                                                                              aTTCGTAACGCTGCCGACGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTa                                              >  2:618005/1‑136 (MQ=255)

                                                                                              aTTCGTAACGCTGCCGACGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTa                                              <  1:618005/136‑1 (MQ=255)

                                                                                                        cTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGc                                >  2:796357/1‑140 (MQ=255)

                                                                                                        cTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGc                                <  1:309442/140‑1 (MQ=255)

                                                                                                          gccgccGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGc                                <  1:969158/138‑1 (MQ=255)

                                                                                                          gccgccGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGc                                >  2:969158/1‑138 (MQ=255)

                                                                                                           ccgccgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGt                             >  2:943220/1‑140 (MQ=255)

                                                                                                             gccgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTaa                           <  1:796357/140‑1 (MQ=255)

                                                                                                             gccgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTaa                           <  1:534467/140‑1 (MQ=255)

                                                                                                             gccgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTaa                           <  1:410740/140‑1 (MQ=255)

                                                                                                                   gCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTTAGCGGTAACACCAg                     >  2:627843/1‑140 (MQ=255)

                                                                                                                                     gcggcgCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCAcacc   >  2:935070/1‑140 (MQ=255)

                                                                                                                                      cgGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCAcacca  <  1:402454/140‑1 (MQ=255)

GCCGTGGTGGGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGTGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAGCAGCACACCA  >  NC_000913/2296281‑2296554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A