Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,358,558	G→A	I96I (ATC→ATT)	yfaU ←	2‑keto‑3‑deoxy‑L‑rhamnonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,358,558	0	G	A	100.0%	159.2 / NA	46	I96I (ATC→ATT)	yfaU	2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/20); total (26/20)

CGCCATGTAATTCTCAATGCGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGAT  >  NC_000913/2358426‑2358679

cgcCATGTAATTCTCAATGCGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTa                                                                                                                    >  1:231925/1‑140 (MQ=255)

             tcAATGCGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGcc                                                                                                       >  1:274206/1‑140 (MQ=255)

              cAATGCGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCa                                                                                                      <  1:314194/140‑1 (MQ=255)

                 tGCGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAata                                                                                                   >  1:89040/1‑140 (MQ=255)

                 tGCGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAata                                                                                                   >  2:424355/1‑140 (MQ=255)

                 tGCGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAata                                                                                                   >  2:233289/1‑140 (MQ=255)

                   cGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCCCCAATATc                                                                                                 <  1:859535/140‑1 (MQ=255)

                    gTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATcc                                                                                                >  2:624334/1‑140 (MQ=255)

                    gTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATcc                                                                                                >  1:782228/1‑140 (MQ=255)

                        ccAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGa                                                                                            <  1:257001/140‑1 (MQ=255)

                                cAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTa                                                                                    >  2:314447/1‑140 (MQ=255)

                                     cGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAg                                                                               >  2:759150/1‑140 (MQ=255)

                                             acTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTAc                                                                       >  2:97718/1‑140 (MQ=255)

                                                 gCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGcc                                                                   >  1:871436/1‑140 (MQ=255)

                                                 gCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATACAGGACTTGTTTAATCAGCGGTTTACTGcc                                                                   >  1:25505/1‑140 (MQ=255)

                                                  ccccGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCt                                                                  >  2:373510/1‑140 (MQ=255)

                                                  ccccGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCt                                                                  >  1:495362/1‑140 (MQ=255)

                                                           cacGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACcgga                                                         >  1:408139/1‑140 (MQ=255)

                                                             cGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACcggacg                                                       >  1:234581/1‑140 (MQ=255)

                                                              gCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACcggacgg                                                      >  2:425547/1‑140 (MQ=255)

                                                                tCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGAt                                                    <  2:234581/140‑1 (MQ=255)

                                                                      tAGGGAGGATAGCGCGTGGCAGACACTACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACggg                                              <  1:624334/140‑1 (MQ=255)

                                                                      tAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACggg                                              <  1:314447/140‑1 (MQ=255)

                                                                      tAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTAGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACggg                                              >  2:797338/1‑140 (MQ=255)

                                                                        gggAGGGTATCACGGGCCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGtt                                            >  2:416834/1‑140 (MQ=255)

                                                                         ggaggaTAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTtg                                           >  2:93226/1‑140 (MQ=255)

                                                                              aTAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTtggctgg                                      >  2:93560/1‑140 (MQ=255)

                                                                                aGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCa                                    <  1:792763/140‑1 (MQ=255)

                                                                                     gTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAggg                               <  2:782228/140‑1 (MQ=255)

                                                                                       ggCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGgcg                             >  1:49999/1‑140 (MQ=255)

                                                                                        gCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGgcgc                            <  1:424355/140‑1 (MQ=255)

                                                                                          aGACCCCACCTGACGTCCCTGTTGGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATAGCCGGGACTTTTTTAACCAGCGGTTTCCTGCCTTCCCCCGGACGGATCACGGTTTGGCGGGCATAGGGCGCTa                          <  2:62501/140‑1 (MQ=255)

                                                                                          aGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTa                          <  2:495362/140‑1 (MQ=255)

                                                                                          aGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTa                          <  2:408139/140‑1 (MQ=255)

                                                                                            acacCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTAcc                        >  1:788159/1‑140 (MQ=255)

                                                                                            acacCACCTGACGTGCCTGTTAGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGCCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTAcc                        <  1:797338/140‑1 (MQ=255)

                                                                                            acacCACCCGACGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTAcc                        <  2:51840/140‑1 (MQ=255)

                                                                                                      aCGTGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCt              <  2:112764/140‑1 (MQ=255)

                                                                                                         tGCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgat           <  1:759150/140‑1 (MQ=255)

                                                                                                          gCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgatg          >  1:170425/1‑140 (MQ=255)

                                                                                                          gCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgat           <  2:32545/139‑1 (MQ=255)

                                                                                                          gCCTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgat           >  1:32545/1‑139 (MQ=255)

                                                                                                           ccTGTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgatga         >  2:694286/1‑140 (MQ=255)

                                                                                                              gTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATaa      <  1:513263/140‑1 (MQ=255)

                                                                                                              gTTCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATaa      <  1:93226/140‑1 (MQ=255)

                                                                                                                tCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGc               <  1:468670/129‑1 (MQ=255)

                                                                                                                tCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGc               >  2:468670/1‑129 (MQ=255)

                                                                                                                tCGGCAGTATCGACCATCGGAATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAg    <  1:233289/140‑1 (MQ=255)

                                                                                                                  ggCGGTATCGACCAGCGGAACCAGTGGGGTTGGCGCCCCAATATCCAGGGCTTGTTTAATCGGCGGTTTTCTGCCTTCAACCGAAGGGATCACGGGTTGGCTGGCATAGGGCGTTACCGCCTGTAGCTGATGATAAAGAt  <  2:170425/140‑1 (MQ=255)

CGCCATGTAATTCTCAATGCGTCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGAT  >  NC_000913/2358426‑2358679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A