Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 163 52.9 1786516 99.3% 1774010 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,366,570 G→A A221T (GCC→ACC)  arnB → UDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,366,5700GA100.0% 160.5 / NA 48A221T (GCC→ACC) arnBUDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/29);  total (19/29)

AAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGCACCTCAACACCCGT  >  NC_000913/2366439‑2366704
                                                                                                                                   |                                                                                                                                      
aaGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATgac                                                                                                                                >  2:452002/1‑140 (MQ=255)
   gTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATgacaga                                                                                                                             <  1:358114/140‑1 (MQ=255)
   gTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATgacaga                                                                                                                             <  1:603153/140‑1 (MQ=255)
    tACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATgacagac                                                                                                                            <  2:150946/140‑1 (MQ=255)
    tACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATgacagac                                                                                                                            <  1:539202/140‑1 (MQ=255)
         cTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAAcc                                                                                                                       >  2:692791/1‑140 (MQ=255)
            ttttttCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATgacagac                                                                                                                            <  2:662154/132‑1 (MQ=255)
            ttttttCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATgacagac                                                                                                                            >  1:662154/1‑132 (MQ=255)
            ttttttCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTgg                                                                                                                    >  2:239611/1‑140 (MQ=255)
              ttttCATTTTATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTgggg                                                                                                                  <  1:515762/140‑1 (MQ=255)
              ttttCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTgggg                                                                                                                  <  2:732196/140‑1 (MQ=255)
                   atttcatGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTg                                                                                                             <  2:182960/140‑1 (MQ=255)
                    tttcatGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGc                                                                                                            >  2:417100/1‑140 (MQ=255)
                         tGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGc                                                                                                       <  1:808335/140‑1 (MQ=255)
                             aTTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGc                                                                                                   >  2:738683/1‑140 (MQ=255)
                              ttAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCGGGCt                                                                                                  <  2:212755/140‑1 (MQ=255)
                              ttAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCt                                                                                                  <  2:604332/140‑1 (MQ=255)
                               tAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTg                                                                                                 >  1:272068/1‑140 (MQ=255)
                                aaaaaTATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGa                                                                                                <  1:104085/140‑1 (MQ=255)
                                aaaaaTATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGa                                                                                                <  2:94968/140‑1 (MQ=255)
                                        tACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCt                                                                                                  <  1:602912/130‑1 (MQ=255)
                                        tACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCt                                                                                                  >  2:602912/1‑130 (MQ=255)
                                              tgCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCgg                                                                                  >  1:490918/1‑140 (MQ=255)
                                              tgCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCgg                                                                                  <  2:455549/140‑1 (MQ=255)
                                              tgCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCgg                                                                                  <  1:452002/140‑1 (MQ=255)
                                              tgCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCgg                                                                                  >  1:112136/1‑140 (MQ=255)
                                                cTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGa                                                                                                <  1:363368/124‑1 (MQ=255)
                                                cTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGa                                                                                                >  2:363368/1‑124 (MQ=255)
                                                  gAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCta                                                                              <  1:890625/140‑1 (MQ=255)
                                                  gAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCta                                                                              <  1:239611/140‑1 (MQ=255)
                                                  gAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCta                                                                              <  1:417100/140‑1 (MQ=255)
                                                   aaGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCtat                                                                             >  2:363222/1‑140 (MQ=255)
                                                        ggCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTa                                                                        >  1:160632/1‑140 (MQ=255)
                                                          ccTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACa                                                                      >  1:15468/1‑140 (MQ=255)
                                                                        aTAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATAtt                                                        >  2:230481/1‑140 (MQ=255)
                                                                        aTAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATAtt                                                        >  1:842758/1‑140 (MQ=255)
                                                                        aTAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATAtt                                                        <  2:490918/140‑1 (MQ=255)
                                                                        aTAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGAAAtt                                                        >  2:476797/1‑140 (MQ=255)
                                                                          aaTGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTaa                                                      >  2:804131/1‑140 (MQ=255)
                                                                                     tGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTg                                           <  1:476797/140‑1 (MQ=255)
                                                                                     tGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTg                                           <  1:738683/140‑1 (MQ=255)
                                                                                     tGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTg                                           <  1:230481/140‑1 (MQ=255)
                                                                                     tGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGACATTAACGCCGCGATTg                                           <  1:726012/140‑1 (MQ=255)
                                                                                              gCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGacac                                  <  1:804131/140‑1 (MQ=255)
                                                                                                     aTGCTGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGt                           <  2:272068/140‑1 (MQ=255)
                                                                                                         tGAAATTTCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCaaa                       >  2:96755/1‑140 (MQ=255)
                                                                                                               ttCACGGTCTGGGTGTCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTagag                 <  2:15468/140‑1 (MQ=255)
                                                                                                                              tCGATACCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGCACCTCAACACCCGt  <  1:692791/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
AAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGCACCTCAACACCCGT  >  NC_000913/2366439‑2366704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A