Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,368,510	G→A	A158T (GCT→ACT)	arnA →	fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,368,510	0	G	A	100.0%	116.7 / NA	34	A158T (GCT→ACT)	arnA	fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/12); total (22/12)

TGGTCGCGCGCCGCTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACG  >  NC_000913/2368374‑2368644

tGGTCGCGCGCCGCTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTa                                                                                                                                     >  1:724410/1‑140 (MQ=255)

tGGTCGCGCGCCGCTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTa                                                                                                                                     >  2:475203/1‑140 (MQ=255)

   tCGCGCGCCGCTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTa                                                                                                                                  <  1:483536/140‑1 (MQ=255)

     gcgcgcCGCTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACg                                                                                                                                <  2:804290/140‑1 (MQ=255)

       gcgcCGCTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCt                                                                                                                              <  1:384719/140‑1 (MQ=255)

       gcgcCGCTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCt                                                                                                                              <  1:475203/140‑1 (MQ=255)

       gcgcCGCTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCt                                                                                                                              <  2:584917/140‑1 (MQ=255)

           cgcTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCt                                                                                                                              >  2:462398/1‑136 (MQ=255)

           cgcTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCt                                                                                                                              <  1:462398/136‑1 (MQ=255)

             cTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGcatca                                                                                                                        <  2:699851/140‑1 (MQ=255)

                     ggTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATtgt                                                                                                                >  1:900953/1‑140 (MQ=255)

                       tGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGc                                                                                                              >  2:954212/1‑140 (MQ=255)

                       tGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGc                                                                                                              >  1:1242114/1‑140 (MQ=255)

                          tGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCAt                                                                                                           >  1:727529/1‑140 (MQ=255)

                          tGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCAt                                                                                                           >  1:374854/1‑140 (MQ=255)

                                aCGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGGCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGGCAGACGATATCGCTATTACGCTGCATCATAAAGTGTGCCATGCcgcg                                                                                                     <  2:513866/140‑1 (MQ=255)

                                   gTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCgcgcgc                                                                                                  <  2:715314/140‑1 (MQ=255)

                                     gAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCa                                                                                                <  2:465444/140‑1 (MQ=255)

                                      aaaCGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAg                                                                                               >  1:700739/1‑140 (MQ=255)

                                         cGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTa                                                                                            <  1:1062129/140‑1 (MQ=255)

                                              aCTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGa                                                                                       <  2:23638/140‑1 (MQ=255)

                                              aCTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGa                                                                                       >  1:304286/1‑140 (MQ=255)

                                               cTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGaa                                                                                      <  2:727529/140‑1 (MQ=255)

                                                 ggCGTTACATTGCACCGAATGGGGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACa                                                                                    >  1:451713/1‑140 (MQ=255)

                                                                   aTGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTaa                                                                  >  1:61151/1‑140 (MQ=255)

                                                                            cGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTaa                                                         >  2:167869/1‑140 (MQ=255)

                                                                            cGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTACATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTaa                                                         >  2:1081130/1‑140 (MQ=255)

                                                                              tGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAata                                                       >  2:1022592/1‑140 (MQ=255)

                                                                              tGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAata                                                       >  1:1228470/1‑140 (MQ=255)

                                                                                cgggtGCGGGGGCCTTTGTGGCGCAACGGCGCTTTGCCATGGCGCAAGACAATACCATTATTCCGCTAAATAAAAATTTGTCCCCCGCCGCCCCCCAGCTACGGAAACAAACATTCCCCGCCATTAAACACGGTAATAtt                                                     <  2:724410/136‑1 (MQ=255)

                                                                                ccGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATAtt                                                     >  1:329625/1‑140 (MQ=255)

                                                                                                        aaCTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAAc                             >  2:265264/1‑140 (MQ=255)

                                                                                                         aCTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACg                            >  1:452080/1‑140 (MQ=255)

                                                                                                          cTGCGCATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGa                           >  2:759871/1‑140 (MQ=255)

                                                                                                           ggcgcTTTGCCATGGCGCAAGACTATATCATTATTCCGCTGCATAAAAATTTGTCCCCCCCCGCGCCCCAGCTACTGAAACAGACTTTCCCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGaa                          <  2:329625/139‑1 (MQ=255)

                                                                                                            gcgcATTGCCATTGCGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAg                         >  1:705512/1‑140 (MQ=255)

                                                                                                                    ccattgcGCCAGACGATATCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGt                 >  2:448142/1‑140 (MQ=255)

                                                                                                                                   atatCACTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACg  >  1:827555/1‑140 (MQ=255)

TGGTCGCGCGCCGCTGAACTGGGTGCTGGTCAACGGTGAAACGGAAACTGGCGTTACATTGCACCGAATGGTGAAACGTGCCGATGCCGGGGCCATTGTGGCGCAACTGCGCATTGCCATTGCGCCAGACGATATCGCTATTACGCTGCATCATAAATTGTGCCATGCCGCGCGCCAGCTACTGGAACAGACATTACCCGCCATTAAACACGGTAATATTCTGGAAATCGCCCAGCGCGAAAACGAAGCCACCTGTTTTGGTCGCAGAACG  >  NC_000913/2368374‑2368644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A