Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,418,918	G→A	R87C (CGC→TGC)	yfcD ←	putative Nudix hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,418,918	0	G	A	100.0%	172.7 / NA	50	R87C (CGC→TGC)	yfcD	putative Nudix hydrolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/24); total (26/24)

TGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCGACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGTCTCGGTACGACGCTGGACCAGAATTTTGCCCATGCCATCATGCACGACG  >  NC_000913/2418785‑2419051

tGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAAcgcgc                                                                                                                                 >  1:198127/1‑140 (MQ=255)

tGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAAcgcgc                                                                                                                                 >  1:470837/1‑140 (MQ=255)

     tGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGAtt                                                                                                                            >  2:868572/1‑140 (MQ=255)

     tGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGAtt                                                                                                                            <  1:393161/140‑1 (MQ=255)

      gCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTc                                                                                                                           >  2:249565/1‑140 (MQ=255)

       cGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAAcgcgcg                                                                                                                                >  1:754824/1‑134 (MQ=255)

       cGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAAcgcgcg                                                                                                                                <  2:754824/134‑1 (MQ=255)

       cGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTcc                                                                                                                          >  2:464660/1‑140 (MQ=255)

       cGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTcc                                                                                                                          >  1:48568/1‑140 (MQ=255)

       cGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTcc                                                                                                                          >  2:315905/1‑140 (MQ=255)

       cGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTcc                                                                                                                          >  1:372653/1‑140 (MQ=255)

       cGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAATTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTcc                                                                                                                          <  2:728705/140‑1 (MQ=255)

         aaGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCcag                                                                                                                        >  1:686580/1‑140 (MQ=255)

         aaGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCcag                                                                                                                        >  2:618335/1‑140 (MQ=255)

               ccGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTg                                                                                                                  >  1:621722/1‑140 (MQ=255)

                   gagagaCGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCa                                                                                                              <  2:394618/140‑1 (MQ=255)

                   gagagaCGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCa                                                                                                              <  2:48568/140‑1 (MQ=255)

                    agagaCGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCAt                                                                                                             <  1:464660/140‑1 (MQ=255)

                    agagaCGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCAt                                                                                                             <  2:686580/140‑1 (MQ=255)

                      agaCGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTTCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCg                                                                                                           >  1:493238/1‑140 (MQ=255)

                        aCGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGc                                                                                                         <  2:245782/140‑1 (MQ=255)

                        aCGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGc                                                                                                         <  1:618335/140‑1 (MQ=255)

                        aCGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGc                                                                                                         <  2:470837/140‑1 (MQ=255)

                        aCGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGc                                                                                                         <  2:478581/140‑1 (MQ=255)

                        aCGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGc                                                                                                         <  1:432731/140‑1 (MQ=255)

                           cAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTg                                                                                                      <  2:198127/140‑1 (MQ=255)

                                        cgcCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCt                                                                                         >  2:659057/1‑140 (MQ=255)

                                           cccAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCg                                                                                      >  1:370378/1‑140 (MQ=255)

                                           cccAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCg                                                                                      >  1:45277/1‑140 (MQ=255)

                                            ccAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCgg                                                                                     <  2:372653/140‑1 (MQ=255)

                                             cAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGt                                                                                    <  1:315905/140‑1 (MQ=255)

                                                    ggCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCt                                                                             >  2:131905/1‑140 (MQ=255)

                                                       aaTTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAAc                                                                          <  2:594339/140‑1 (MQ=255)

                                                           tttATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGc                                                                      <  1:868572/140‑1 (MQ=255)

                                                            ttATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGt                                                                                    <  1:298583/125‑1 (MQ=255)

                                                            ttATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGt                                                                                    >  2:298583/1‑125 (MQ=255)

                                                                           aaCTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTaaaa                                                             <  1:449638/133‑1 (MQ=255)

                                                                           aaCTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTaaaa                                                             >  2:449638/1‑133 (MQ=255)

                                                                             cTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTg                                                    >  1:642495/1‑140 (MQ=255)

                                                                                     gCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGTCTCGGTa                                            >  2:225155/1‑140 (MQ=255)

                                                                                          gCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTCTTGTCTCGGTacgacg                                       >  2:381993/1‑140 (MQ=255)

                                                                                               ggggACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAA‑‑CGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGCCTCGGTACGACGCTGGAcc                                <  1:774890/140‑1 (MQ=255)

                                                                                                gggACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGTCTCGGTACGACGCTGGAc                                 <  1:488690/140‑1 (MQ=255)

                                                                                                   acacCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGTCTCGGTACGACGCTGGACCAg                              >  2:23422/1‑140 (MQ=255)

                                                                                                     acCGGCAATGCCCAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGTCTCGGTACGACGCTGGACCAGaa                            >  1:558869/1‑140 (MQ=255)

                                                                                                                ccAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGTCTCGGTACGACGCTGGACCAGAATTTTGCCCATg                 <  1:659057/140‑1 (MQ=255)

                                                                                                                ccAACTCTTCTTCCGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGTCTCGGTACGACGCTGGACCAGAATTTTGCCCATg                 <  1:88154/140‑1 (MQ=255)

                                                                                                                            ccGCTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTTACATGCCGGGTAAAAAGTCTTTTGTCTCGGTACGACGCTGGACCAGAATTTTGCCCATGCCATCATGCacg     <  2:621722/140‑1 (MQ=255)

                                                                                                                               cTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTGCACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGTCTCGGTACGACGCTGGACCAGAATTTTGCCCATGCCATCATGCacgacg  >  2:226422/1‑140 (MQ=255)

                                                                                                                               cTTCGCAACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGTCTCGGTACGACGCTGGACCAGAATTTTGCCCATGCCATCATGCacgacg  >  2:694928/1‑140 (MQ=255)

TGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCTCGGCAAAGGGGACACCGGCAATGCCCAACTCTTCTTCCGCTTCGCGACGCGCGGATTCCAGCAGTTGCTCATCGGCCTGGACTACACCGCCTGCGGTCGCATCTAACATGCCGGGTAAAAAGTCTTTTGTCTCGGTACGACGCTGGACCAGAATTTTGCCCATGCCATCATGCACGACG  >  NC_000913/2418785‑2419051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A