Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,544,344	G→A	R94C (CGT→TGT)	ucpA ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,544,344	0	G	A	97.4%	127.6 / ‑4.1	39	R94C (CGT→TGT)	ucpA	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (20/18); total (21/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

ATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACAACCGCCGTACAGCGATGACCACGACCA  >  NC_000913/2544208‑2544474

atcatcACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGacaaca                                                                                                                                 <  1:440938/140‑1 (MQ=255)

           gCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGt                                                                                                                      <  1:821394/140‑1 (MQ=255)

           gCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGt                                                                                                                      <  1:695239/140‑1 (MQ=255)

            cGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGtt                                                                                                                     >  1:148017/1‑140 (MQ=255)

            cGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGtt                                                                                                                     >  1:371585/1‑140 (MQ=255)

             gACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTa                                                                                                                    <  1:51273/140‑1 (MQ=255)

             gACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTa                                                                                                                    >  1:460565/1‑140 (MQ=255)

              aCCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTAt                                                                                                                   >  2:488715/1‑140 (MQ=255)

                  tCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCAc                                                                                                               >  1:120148/1‑140 (MQ=255)

                  tCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCAc                                                                                                               >  2:213517/1‑140 (MQ=255)

                      tGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTa                                                                                                                    >  1:812199/1‑131 (MQ=255)

                      tGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTa                                                                                                                    <  2:812199/131‑1 (MQ=255)

                        cGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGat                                                                                                         <  2:176169/140‑1 (MQ=255)

                                  tctcCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATgcgc                                                                                               <  2:840459/140‑1 (MQ=255)

                                     ccGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCct                                                                                            >  2:691819/1‑140 (MQ=255)

                                             aCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTc                                                                                    >  2:449152/1‑140 (MQ=255)

                                                    tCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTcgcg                                                                             <  2:408256/140‑1 (MQ=255)

                                                    tCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTcgcg                                                                             <  1:213517/140‑1 (MQ=255)

                                                    tCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTcgcg                                                                             <  1:436660/140‑1 (MQ=255)

                                                    tCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTcgcg                                                                             <  2:148017/140‑1 (MQ=255)

                                                                 tACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTcgcg                                                                             >  1:52543/1‑127 (MQ=255)

                                                                 tACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTcgcg                                                                             <  2:52543/127‑1 (MQ=255)

                                                                       tttAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTa                                                          >  2:232158/1‑140 (MQ=255)

                                                                               tGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGcc                                                  <  2:371585/140‑1 (MQ=255)

                                                                               tGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGcc                                                  >  1:34153/1‑140 (MQ=255)

                                                                                gATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCg                                                 >  1:426524/1‑140 (MQ=255)

                                                                                   gTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGt                                              >  2:212654/1‑140 (MQ=255)

                                                                                   gTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGt                                              <  1:258645/140‑1 (MQ=255)

                                                                                        tatGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGc                                         >  1:709213/1‑140 (MQ=255)

                                                                                           ggAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGcaca                                      <  1:449152/140‑1 (MQ=255)

                                                                                              aaTCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCg                                   >  1:256775/1‑140 (MQ=255)

                                                                                                   cggtCGTCACCGTTAATAACGAGAAATCCGCCCAGCCGAAAACCGCCTGCGTTATTCACCAGAATACAAATCCCCCTTTTTTTTCCCTTCGCGGTTTTGATGGCGGGGGCAACCGCCGCGGGGTCACGCCCACCGGCGAc                              <  2:120148/137‑1 (MQ=255)

                                                                                                     aTCGTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACaa                            <  1:488715/140‑1 (MQ=255)

                                                                                                        gTCATCGCTCATATCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACAAccg                         <  1:691819/140‑1 (MQ=255)

                                                                                                                  atatCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACAACCGCCGTACAGCg               <  1:212654/140‑1 (MQ=255)

                                                                                                                  atatCGAGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACAACCGCCGTACAGCg               <  1:162637/140‑1 (MQ=255)

                                                                                                                        aGGAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACAACCGCCGTACAGCGATgacc         >  2:364386/1‑140 (MQ=255)

                                                                                                                          gAAACTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACAACCGCCGTACAGCGATgaccac       >  2:845866/1‑140 (MQ=255)

                                                                                                                              cTGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACAACCGCCGTACAGCGATgaccacgacc   >  1:507097/1‑140 (MQ=255)

                                                                                                                               tGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACAACCGCCGTACAGCGATgaccacgacca  >  1:38937/1‑140 (MQ=255)

                                                                                                                               tGCCCAGACAACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACAACCGCCGTACAGCGATgaccacgacca  >  1:295764/1‑140 (MQ=255)

ATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTTTTTCCTTCGCGCGTTTGATAGCTGCGGCTACCGACGCCGGGTCACGCACATCGGCGACAACCGCCGTACAGCGATGACCACGACCA  >  NC_000913/2544208‑2544474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A