Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,573,889	C→T	A139A (GCG→GCA)	eutT ←	putative ethanolamine utilization cobalamin adenosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,573,889	0	C	T	100.0%	132.2 / NA	38	A139A (GCG→GCA)	eutT	putative ethanolamine utilization cobalamin adenosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/22); total (16/22)

TGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGACCATTTTTTCCGCCGACAGGTGAGT  >  NC_000913/2573755‑2574023

tGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCGCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTgccag                                                                                                                                   >  1:804346/1‑140 (MQ=255)

tGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTgccag                                                                                                                                   <  1:604356/140‑1 (MQ=255)

   tCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTgccagcca                                                                                                                                >  1:443609/1‑140 (MQ=255)

      agaTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACgg                                                                                                                             >  1:537534/1‑140 (MQ=255)

        aTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCt                                                                                                                           >  2:155307/1‑140 (MQ=255)

           gcgcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGcc                                                                                                                        <  1:17221/140‑1 (MQ=255)

            cgcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCa                                                                                                                       >  2:626139/1‑140 (MQ=255)

                 cGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGtt                                                                                                                  >  2:714760/1‑140 (MQ=255)

                 cGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGtt                                                                                                                  >  2:747301/1‑140 (MQ=255)

                       atgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCa                                                                                                            >  1:68957/1‑140 (MQ=255)

                          agaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGtt                                                                                                         <  1:752726/140‑1 (MQ=255)

                                   aTGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCa                                                                                                <  1:531945/140‑1 (MQ=255)

                                     gCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGc                                                                                              <  1:747301/140‑1 (MQ=255)

                                        gATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGTAGCcac                                                                                           <  2:642754/140‑1 (MQ=255)

                                             tCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGc                                                                                      <  2:349141/140‑1 (MQ=255)

                                              cGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGcc                                                                                     >  2:408212/1‑140 (MQ=255)

                                                tCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAg                                                                                   <  1:714760/140‑1 (MQ=255)

                                                    aaaGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCa                                                                               >  2:5765/1‑140 (MQ=255)

                                                       gCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATg                                                                            <  1:252076/140‑1 (MQ=255)

                                                          cGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCTCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTg                                                                         <  2:443609/140‑1 (MQ=255)

                                                          cGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCGGCGCAATGGTg                                                                         <  2:663334/140‑1 (MQ=255)

                                                          cGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTg                                                                         <  1:626139/140‑1 (MQ=255)

                                                          cGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTg                                                                         <  2:180607/140‑1 (MQ=255)

                                                          cGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTg                                                                         <  2:621100/140‑1 (MQ=255)

                                                          cGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTg                                                                         <  2:763263/140‑1 (MQ=255)

                                                          cGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTg                                                                         <  1:120216/140‑1 (MQ=255)

                                                          cGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCACATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCAACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTg                                                                         <  2:302085/140‑1 (MQ=255)

                                                                                       cccAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAcg                                            <  1:155307/140‑1 (MQ=255)

                                                                                            cgcATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGAt                                       >  1:416127/1‑140 (MQ=255)

                                                                                                    cgcgcATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTg                               >  1:466765/1‑140 (MQ=255)

                                                                                                     gcgcATAATGTTGCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTgg                              <  2:68957/140‑1 (MQ=255)

                                                                                                                 gCCAAGACGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGACCAtttttt                  >  2:281837/1‑140 (MQ=255)

                                                                                                                       aCGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGACCATTTTTTccgccg            >  2:135447/1‑140 (MQ=255)

                                                                                                                       aCGCGAACGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGACCATTTTTTccgccg            <  1:519337/140‑1 (MQ=255)

                                                                                                                         gcgAACGGATATCTGCCAGCCACGGCTGCCACGGTTACGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGACCATTTTTTCCGCCGAc          >  2:673010/1‑140 (MQ=255)

                                                                                                                             aCGGATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGACCATTTTTTCCGCCGACAGGt      <  1:679650/140‑1 (MQ=255)

                                                                                                                                gATATCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGACCATTTTTTCCGCCGACAGGTGAg   <  2:416127/140‑1 (MQ=255)

                                                                                                                                 atatCTGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGACCATTTTTTCCGCCGACAGGTGAGt  >  1:289062/1‑140 (MQ=255)

TGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGACCATTTTTTCCGCCGACAGGTGAGT  >  NC_000913/2573755‑2574023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A