Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,575,920	A→G	intergenic (‑115/+178)	eutS ← / ← maeB	putative structural protein, ethanolamine utilization microcompartment/malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,575,920	0	A	G	100.0%	116.3 / NA	33	intergenic (‑115/+178)	eutS/maeB	putative structural protein, ethanolamine utilization microcompartment/malate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/16); total (17/16)

TGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAAC  >  NC_000913/2575783‑2576053

tGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGtt                                                                                                                                     <  2:511087/140‑1 (MQ=255)

      atgCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCtgt                                                                                                                               <  2:207662/140‑1 (MQ=255)

         cGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCg                                                                                                                                   <  2:478641/133‑1 (MQ=255)

         cGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCg                                                                                                                                   >  1:478641/1‑133 (MQ=255)

         cGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTgtggt                                                                                                                            >  1:776960/1‑140 (MQ=255)

                tATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTgtgg                                                                                                                             >  2:215492/1‑132 (MQ=255)

                tATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTgtgg                                                                                                                             <  1:215492/132‑1 (MQ=255)

                tATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGCAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATaaa                                                                                                                     <  2:223769/140‑1 (MQ=255)

                      tGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCa                                                                                                               >  1:118000/1‑140 (MQ=255)

                      tGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCa                                                                                                               >  1:843258/1‑140 (MQ=255)

                      tGAGTCGCCGCCTGAGAAAAACTTTAGTGTATCGTGACAATTTTTCGTATGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGTGGTAGATCACTAAGAATGGAGAGATGATCGATGTGGTGCATAAACTTTCa                                                                                                               >  2:171930/1‑140 (MQ=255)

                        aGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCtgtg                                                                                                                              >  2:628989/1‑123 (MQ=255)

                        aGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCtgtg                                                                                                                              <  1:628989/123‑1 (MQ=255)

                        aGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCata                                                                                                             >  1:925999/1‑140 (MQ=255)

                                 cTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCa                                                                                                    <  2:52060/140‑1 (MQ=255)

                                           cTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATataaa                                                                                          <  2:925999/140‑1 (MQ=255)

                                                        tGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATataaa                                                                                          <  2:226224/127‑1 (MQ=255)

                                                        tGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATataaa                                                                                          >  1:226224/1‑127 (MQ=255)

                                                        tGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCg                                                                             <  1:838173/140‑1 (MQ=255)

                                                        tGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCg                                                                             >  2:216036/1‑140 (MQ=255)

                                                          aCAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCgag                                                                           <  2:118000/140‑1 (MQ=255)

                                                          aCAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCgag                                                                           <  2:824907/140‑1 (MQ=255)

                                                          aCAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCgag                                                                           <  1:271964/140‑1 (MQ=255)

                                                            aaTTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGat                                                                         <  2:953404/140‑1 (MQ=255)

                                                            aaTTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGat                                                                         <  1:216036/140‑1 (MQ=255)

                                                                       agagTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGAc                                                              <  2:843258/140‑1 (MQ=255)

                                                                                   aaaaTTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTtta                                                  >  1:308628/1‑140 (MQ=255)

                                                                                                             tGAGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCtt                        >  1:616293/1‑140 (MQ=255)

                                                                                                               aGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTa                      >  1:472715/1‑140 (MQ=255)

                                                                                                               aGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTa                      >  2:741965/1‑140 (MQ=255)

                                                                                                               aGGTAGATCACTAAGAATGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTa                      >  1:104526/1‑140 (MQ=255)

                                                                                                                               aTGGAGAGATGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCt      >  1:1071528/1‑140 (MQ=255)

                                                                                                                                   agagaTGTTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAAc  >  2:973412/1‑140 (MQ=255)

TGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAAGCTTTACTAATATATTGCAGCTAAAC  >  NC_000913/2575783‑2576053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A