Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,618,396	C→T	S127S (TCG→TCA)	hda ←	inibitor of reinitiation of DNA replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,618,396	0	C	T	100.0%	200.2 / NA	57	S127S (TCG→TCA)	hda	inibitor of reinitiation of DNA replication
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/30); total (27/30)

CCTGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGT  >  NC_000913/2618260‑2618530

ccTGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGAt                                                                                                                                     >  2:443709/1‑140 (MQ=255)

  tGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTc                                                                                                                                   >  2:634521/1‑140 (MQ=255)

    cAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCa                                                                                                                                 <  1:250606/140‑1 (MQ=255)

         tATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGaa                                                                                                                              >  2:881059/1‑138 (MQ=255)

         tATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGaa                                                                                                                              <  1:881059/138‑1 (MQ=255)

         tATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAAtt                                                                                                                            <  2:700114/140‑1 (MQ=255)

         tATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAAtt                                                                                                                            <  2:655874/140‑1 (MQ=255)

                  caGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTag                                                                                                                   >  2:270623/1‑140 (MQ=255)

                  caGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTag                                                                                                                   >  2:289459/1‑140 (MQ=255)

                    gAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTagag                                                                                                                 <  2:726874/140‑1 (MQ=255)

                         tGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGTTTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGa                                                                                                            >  1:291748/1‑140 (MQ=255)

                           gCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGaaa                                                                                                          >  2:855424/1‑140 (MQ=255)

                               cAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCg                                                                                                      >  2:580542/1‑140 (MQ=255)

                                  tttGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCa                                                                                                   <  2:369369/140‑1 (MQ=255)

                                  tttGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCa                                                                                                   <  1:58222/140‑1 (MQ=255)

                                  tttGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCa                                                                                                   <  1:829113/140‑1 (MQ=255)

                                   ttGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCAt                                                                                                  <  2:267323/140‑1 (MQ=255)

                                   ttGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCAt                                                                                                  >  1:55290/1‑140 (MQ=255)

                                          tCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCcac                                                                                           <  1:855424/140‑1 (MQ=255)

                                              cccccAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACt                                                                                       >  2:462799/1‑140 (MQ=255)

                                              cccccAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACt                                                                                       >  2:330438/1‑140 (MQ=255)

                                              cccccAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACt                                                                                       >  2:134433/1‑140 (MQ=255)

                                                  cAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATc                                                                                   <  1:134433/140‑1 (MQ=255)

                                                  cAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATc                                                                                   <  1:775536/140‑1 (MQ=255)

                                                   agtcgagtcgCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCg                                                                                  >  1:647255/1‑140 (MQ=255)

                                                   agtcgagtcgCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCg                                                                                  >  2:686620/1‑140 (MQ=255)

                                                        agtcgCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACGCATCGCCTGc                                                                             >  1:612521/1‑140 (MQ=255)

                                                          tcgCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCaa                                                                           <  2:647255/140‑1 (MQ=255)

                                                           cgcgacgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAAt                                                                          <  1:404846/140‑1 (MQ=255)

                                                           cgcgacgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAAt                                                                          <  1:634521/140‑1 (MQ=255)

                                                             cgacgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGc                                                                                 <  1:262272/131‑1 (MQ=255)

                                                             cgacgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGc                                                                                 >  2:262272/1‑131 (MQ=255)

                                                             cgacgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATac                                                                        >  1:724975/1‑140 (MQ=255)

                                                              gacgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATaca                                                                       <  1:270623/140‑1 (MQ=255)

                                                              gacgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATaca                                                                       <  1:289459/140‑1 (MQ=255)

                                                                cgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACt                                                                     <  2:55290/140‑1 (MQ=255)

                                                                cgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACt                                                                     <  1:67447/140‑1 (MQ=255)

                                                                  cgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAAt                                                                          <  2:256287/133‑1 (MQ=255)

                                                                  cgaGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAAt                                                                          >  1:256287/1‑133 (MQ=255)

                                                                   gagaTCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCaa                                                                  >  1:354019/1‑140 (MQ=255)

                                                                    agaTCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAAt                                                                 <  2:724975/140‑1 (MQ=255)

                                                                    agaTCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAAt                                                                 <  1:330438/140‑1 (MQ=255)

                                                                    agaTCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGACGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAAt                                                                 <  2:612521/140‑1 (MQ=255)

                                                                                 ccAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAg                                                    >  1:753763/1‑140 (MQ=255)

                                                                                        cAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCg                                             >  2:161234/1‑140 (MQ=255)

                                                                                          aCTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGAc                                           <  1:480709/140‑1 (MQ=255)

                                                                                          aCTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGAc                                           >  2:232258/1‑140 (MQ=255)

                                                                                            tGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACaa                                         <  1:460040/140‑1 (MQ=255)

                                                                                            tGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACaa                                         <  1:509539/140‑1 (MQ=255)

                                                                                               cgcgGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATg                                      <  1:686620/140‑1 (MQ=255)

                                                                                                 cgGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCt                                    <  1:232258/140‑1 (MQ=255)

                                                                                                                              gtgtTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAAc       >  1:850677/1‑140 (MQ=255)

                                                                                                                               tgtTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGaa            >  2:204546/1‑134 (MQ=255)

                                                                                                                               tgtTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGaa            <  1:204546/134‑1 (MQ=255)

                                                                                                                               tgtTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAAcc      >  1:280392/1‑140 (MQ=255)

                                                                                                                               tgtTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAAcc      >  1:724503/1‑140 (MQ=255)

                                                                                                                                gtTTTGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCa     >  2:499129/1‑140 (MQ=255)

                                                                                                                                   ttGCCTGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGt  >  1:866301/1‑140 (MQ=255)

CCTGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGT  >  NC_000913/2618260‑2618530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A