Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,647,344	C→T	L1648L (CTG→CTA)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,647,344	0	C	T	100.0%	150.8 / NA	44	L1648L (CTG→CTA)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/20); total (24/20)

GACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATC  >  NC_000913/2647209‑2647475

gACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGAt                                                                                                                                 >  1:571034/1‑140 (MQ=255)

      aTGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAg                                                                                                                           >  1:373322/1‑140 (MQ=255)

      aTGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAg                                                                                                                           >  1:673498/1‑140 (MQ=255)

      aTGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAg                                                                                                                           >  1:558961/1‑140 (MQ=255)

               tAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCgg                                                                                                                  >  2:431553/1‑140 (MQ=255)

                 gagGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTc                                                                                                                >  2:206536/1‑140 (MQ=255)

                 gagGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTc                                                                                                                >  2:680676/1‑140 (MQ=255)

                   ggCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTcgc                                                                                                              <  1:431553/140‑1 (MQ=255)

                      cAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCTCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTcgcc                                                                                                             <  2:813335/138‑1 (MQ=255)

                      cAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCTCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTcgcc                                                                                                             >  1:813335/1‑138 (MQ=255)

                        gagTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTcgcccgcc                                                                                                         >  2:241712/1‑140 (MQ=255)

                             tATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTgg                                                                                                    <  1:680676/140‑1 (MQ=255)

                                  gCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAac                                                                                               <  2:490357/140‑1 (MQ=255)

                                     ccccACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacata                                                                                            >  1:802276/1‑140 (MQ=255)

                                       ccACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacataca                                                                                          <  1:347818/140‑1 (MQ=255)

                                              gCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGaa                                                                                                >  2:593677/1‑127 (MQ=255)

                                              gCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGaa                                                                                                <  1:593677/127‑1 (MQ=255)

                                                       aGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCgg                                                                          <  2:802276/140‑1 (MQ=255)

                                                       aGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCgg                                                                          >  2:807540/1‑140 (MQ=255)

                                                         gagaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGtt                                                                        >  2:389004/1‑140 (MQ=255)

                                                          agaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTg                                                                       <  1:241712/140‑1 (MQ=255)

                                                            aaaGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCg                                                                     <  2:673498/140‑1 (MQ=255)

                                                            aaaGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCg                                                                     <  1:246346/140‑1 (MQ=255)

                                                             aaGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCgg                                                                    <  2:667194/140‑1 (MQ=255)

                                                              aGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGt                                                                   >  2:498623/1‑140 (MQ=255)

                                                                        tGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACg                                                         <  2:558961/140‑1 (MQ=255)

                                                                                    ccAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCa                                             >  1:630623/1‑140 (MQ=255)

                                                                                        cagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAccgc                                         >  1:753709/1‑140 (MQ=255)

                                                                                        cagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAccgc                                         >  1:442144/1‑140 (MQ=255)

                                                                                        cagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAccgc                                         <  1:206536/140‑1 (MQ=255)

                                                                                         agcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAccgcc                                        >  2:118267/1‑140 (MQ=255)

                                                                                         agcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAccgcc                                        <  2:442144/140‑1 (MQ=255)

                                                                                           ccGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAccgcccg                                      >  1:527849/1‑140 (MQ=255)

                                                                                             gcgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCcgcg                                    <  2:86913/140‑1 (MQ=255)

                                                                                             gcgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCcgcg                                    <  1:389004/140‑1 (MQ=255)

                                                                                                  tGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAccgcc                                        >  2:206269/1‑131 (MQ=255)

                                                                                                  tGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAccgcc                                        <  1:206269/131‑1 (MQ=255)

                                                                                                            gCGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCg                     <  2:373322/140‑1 (MQ=255)

                                                                                                             cGAGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCGt                    >  1:540954/1‑140 (MQ=255)

                                                                                                               aGGCATTTACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCGTTa                  <  1:498623/140‑1 (MQ=255)

                                                                                                                    tttACGGTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCGTTACCGGt             >  1:288963/1‑140 (MQ=255)

                                                                                                                         ggTCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCGTTACCGGTTGGTa        <  2:753709/140‑1 (MQ=255)

                                                                                                                           tCTGACAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCGTTACCGGTTGGTAtt      >  1:675498/1‑140 (MQ=255)

                                                                                                                               aCAATCAGTAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCGTTACCGGTTGGTATtcatc  >  1:185379/1‑140 (MQ=255)

GACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATC  >  NC_000913/2647209‑2647475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A