Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,648,364	T→C	V1308V (GTA→GTG)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,648,364	0	T	C	100.0%	526.2 / NA	145	V1308V (GTA→GTG)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (59/86); total (59/86)

Only 100 of 145 total aligned reads displayed.

CCCATGGTTTTCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCG  >  NC_000913/2648227‑2648499

 ccATGGTTTTCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACg                                                                                                                                      <  1:107610/140‑1 (MQ=255)

    tGGTTTTCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACCGGCCAACACCAGCGCAGCGTAAGACTGCACGGCg                                                                                                                                   <  2:2135678/140‑1 (MQ=255)

     ggTTTTCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGa                                                                                                                                  >  2:311887/1‑140 (MQ=255)

     ggTTTTCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGa                                                                                                                                  >  2:2323162/1‑140 (MQ=255)

      gTTTTCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGaa                                                                                                                                 <  2:2386462/140‑1 (MQ=255)

        tttCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAAtt                                                                                                                               >  2:75461/1‑140 (MQ=255)

        tttCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAAtt                                                                                                                               >  1:250153/1‑140 (MQ=255)

          tCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTa                                                                                                                             <  2:1267479/140‑1 (MQ=255)

          tCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTa                                                                                                                             <  1:710652/140‑1 (MQ=255)

           cAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTAc                                                                                                                            <  2:932927/140‑1 (MQ=255)

           cAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTAc                                                                                                                            <  2:1244446/140‑1 (MQ=255)

           cAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTAc                                                                                                                            >  2:1166641/1‑140 (MQ=255)

           cAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTACACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTAc                                                                                                                            >  1:186686/1‑140 (MQ=255)

            aGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACt                                                                                                                           <  1:2280307/140‑1 (MQ=255)

            aGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACt                                                                                                                           <  2:1464144/140‑1 (MQ=255)

              cgcAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAAtt                                                                                                                               >  1:2259405/1‑134 (MQ=255)

               gcAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGc                                                                                                                        >  2:1696192/1‑140 (MQ=255)

                cAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCt                                                                                                                       <  1:650833/140‑1 (MQ=255)

                cAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCt                                                                                                                       >  2:51904/1‑140 (MQ=255)

                cAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCt                                                                                                                       <  2:2410920/140‑1 (MQ=255)

                cAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCt                                                                                                                       <  1:1996244/140‑1 (MQ=255)

                cAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCt                                                                                                                       <  2:1832042/140‑1 (MQ=255)

                cAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCt                                                                                                                       <  2:1597094/140‑1 (MQ=255)

                cAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGAATGCACGGCGAATTTACTGGCt                                                                                                                       <  2:2154523/140‑1 (MQ=255)

                cAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCATAAGACTGCACGGCGAATTTACTGGCt                                                                                                                       <  2:1332310/140‑1 (MQ=255)

                  aCGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCttt                                                                                                                     <  1:1081942/140‑1 (MQ=255)

                          ttGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTAt                                                                                                             <  1:2323162/140‑1 (MQ=255)

                            gcagcaGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATcc                                                                                                           <  1:641316/140‑1 (MQ=255)

                            gcagcaGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATcc                                                                                                           <  1:75461/140‑1 (MQ=255)

                            gcagcaGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATcc                                                                                                           <  2:1497445/140‑1 (MQ=255)

                            gcagcaGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATcc                                                                                                           <  2:2250364/140‑1 (MQ=255)

                               gcagcGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCcgcg                                                                                                        >  2:837583/1‑140 (MQ=255)

                                 agcGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTa                                                                                                      <  1:1520993/140‑1 (MQ=255)

                                        aaCCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATc                                                                                               <  1:920135/140‑1 (MQ=255)

                                        aaCCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATc                                                                                               <  1:1696192/140‑1 (MQ=255)

                                        aaCCAGAAGCGGCATCTACACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATc                                                                                               <  2:186686/140‑1 (MQ=255)

                                             gAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGAcat                                                                                          <  2:2241417/140‑1 (MQ=255)

                                              aaGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGAcatc                                                                                         >  1:1834986/1‑140 (MQ=255)

                                              aaGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGAcatc                                                                                         <  2:1711372/140‑1 (MQ=255)

                                               aGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGAcatca                                                                                        <  2:672095/140‑1 (MQ=255)

                                                gCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTa                                                                                                      <  2:851375/125‑1 (MQ=255)

                                                gCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTa                                                                                                      >  1:851375/1‑125 (MQ=255)

                                                gCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGAcatcat                                                                                       >  1:2155949/1‑140 (MQ=255)

                                                  ggCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGc                                                                                     >  1:452355/1‑140 (MQ=255)

                                                   gCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGcc                                                                                    <  1:922691/140‑1 (MQ=255)

                                                   gCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGcc                                                                                    <  1:311887/140‑1 (MQ=255)

                                                    cATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGccc                                                                                   >  2:2283066/1‑140 (MQ=255)

                                                      tCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCgg                                                                                 <  1:1252059/140‑1 (MQ=255)

                                                      tCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCgg                                                                                 <  2:692221/140‑1 (MQ=255)

                                                      tCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCgg                                                                                 <  2:711651/140‑1 (MQ=255)

                                                      tCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCgg                                                                                 <  1:1593893/140‑1 (MQ=255)

                                                      tCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCgg                                                                                 <  1:1925276/140‑1 (MQ=255)

                                                      tCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCgg                                                                                 <  2:2155949/140‑1 (MQ=255)

                                                      tCTGCACGATGCTCACAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCgg                                                                                 <  1:2283642/140‑1 (MQ=255)

                                                        tGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGAt                                                                               >  2:587310/1‑140 (MQ=255)

                                                         gCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATc                                                                              <  1:114790/140‑1 (MQ=255)

                                                            cGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTg                                                                           >  2:1431547/1‑140 (MQ=255)

                                                             gATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCgg                                                                                 >  1:1276768/1‑133 (MQ=255)

                                                             gATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCgg                                                                                 <  2:1276768/133‑1 (MQ=255)

                                                              aTGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCgg                                                                                 <  2:301429/132‑1 (MQ=255)

                                                                gCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTaaa                                                                       <  1:1205672/140‑1 (MQ=255)

                                                                 ttCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGccc                                                                                   <  1:832677/126‑1 (MQ=255)

                                                                 ttCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGccc                                                                                   >  2:832677/2‑127 (MQ=255)

                                                                  tCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATa                                                                     >  1:2106762/1‑140 (MQ=255)

                                                                  tCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATa                                                                     >  2:1899183/1‑140 (MQ=255)

                                                                  tCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATa                                                                     >  1:1764919/1‑140 (MQ=255)

                                                                   cccAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAg                                                                    <  2:1960051/140‑1 (MQ=255)

                                                                     cAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAgcg                                                                  <  2:2106762/140‑1 (MQ=255)

                                                                           tCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGcagcag                                                            >  1:790291/1‑140 (MQ=255)

                                                                             aCGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGAc                                                          <  1:1750948/140‑1 (MQ=255)

                                                                                  gcgcACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCa                                                     >  2:388520/1‑140 (MQ=255)

                                                                                  gcgcACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCa                                                     >  1:2159520/1‑140 (MQ=255)

                                                                                  gcgcACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCa                                                     >  1:973875/1‑140 (MQ=255)

                                                                                   cgcACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCAt                                                    >  1:1164893/1‑140 (MQ=255)

                                                                                       cccAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTAcc                                                <  1:691399/140‑1 (MQ=255)

                                                                                           gCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCAt                                                    <  2:1781894/132‑1 (MQ=255)

                                                                                               aGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTaa                                        >  1:382966/1‑140 (MQ=255)

                                                                                                  cTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATgg                                     >  1:483334/1‑140 (MQ=255)

                                                                                                    tttGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCg                                   >  2:853673/1‑140 (MQ=255)

                                                                                                             gggCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCgg                           >  1:1212389/1‑139 (MQ=255)

                                                                                                             gggCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCgg                           <  2:1212389/139‑1 (MQ=255)

                                                                                                             gggCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGCCGTCTGTCGGc                          >  2:441986/1‑140 (MQ=255)

                                                                                                              ggCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCa                         <  1:1074438/140‑1 (MQ=255)

                                                                                                              ggCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCa                         <  1:2066289/140‑1 (MQ=255)

                                                                                                              ggCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCa                         >  1:2117029/1‑140 (MQ=255)

                                                                                                              ggCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCa                         <  2:1643359/140‑1 (MQ=255)

                                                                                                              ggCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCa                         <  2:2374211/140‑1 (MQ=255)

                                                                                                              ggCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCa                         >  1:2374211/1‑140 (MQ=255)

                                                                                                              ggCCAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCa                         <  1:1431547/140‑1 (MQ=255)

                                                                                                                 cAACACCAGCGCAGCGTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGc                      <  1:726073/140‑1 (MQ=255)

                                                                                                                         gcgcagcgTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCgg                           <  2:2030103/127‑1 (MQ=255)

                                                                                                                         gcgcagcgTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAAccc              >  2:865866/1‑140 (MQ=255)

                                                                                                                         gcgcagcgTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAAccc              >  2:746547/1‑140 (MQ=255)

                                                                                                                          cgcagcgTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAACCCt             >  1:867352/1‑140 (MQ=255)

                                                                                                                               cgTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAACCCTGTTCg        >  2:1549034/1‑140 (MQ=255)

                                                                                                                               cgTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAACCCTGTTCg        >  1:2241586/1‑140 (MQ=255)

                                                                                                                               cgTAAGACTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAACCCTGTTCg        >  2:1996000/1‑140 (MQ=255)

                                                                                                                                     aCTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTgcg  <  2:128359/140‑1 (MQ=255)

                                                                                                                                     aCTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTgcg  <  2:1164893/140‑1 (MQ=255)

                                                                                                                                     aCTGCACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTgcg  <  1:1452222/140‑1 (MQ=255)

CCCATGGTTTTCAGCGCAACGCCAAGTTGCAGCAGCGGTAAACCAGAAGCGGCATCTGCACGATGCTCCCAGATTTCACGCAGCGCACCCAGCGGAGCCTTTTGCTGACGGGCCAACACCAGCGCAGCGTAAGACTGTACGGCGAATTTACTGGCTTTGAGATTATCCGCGTACGGGATCGACATCATGCCCGGATCTTGTAAATAGCGCAGCAGACGCTCATTACCCCGGTTAATGGCGTCTGTCGGCACGCTGTAACCCTGTTCGCCTGCG  >  NC_000913/2648227‑2648499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A