Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,691,889	C→T	A1219T (GCA→ACA)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,691,889	0	C	T	97.5%	132.7 / ‑4.9	40	A1219T (GCA→ACA)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (23/16); total (24/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGG  >  NC_000913/2691753‑2692019

acacGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGtcac                                                                                                                                 >  2:91926/1‑140 (MQ=255)

acacGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTCTGCCGAAGTTATCGACATAGCGCAGTGtcac                                                                                                                                 >  1:161478/1‑140 (MQ=255)

 cacGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGtcacc                                                                                                                                <  2:858243/140‑1 (MQ=255)

  acGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGtcacca                                                                                                                               >  2:738285/1‑140 (MQ=255)

                           gTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAg                                                                                                      >  1:672914/1‑140 (MQ=255)

                           gTGACTCGACCACTTTCACTCGTGACTGCCATAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAg                                                                                                      >  2:439117/1‑140 (MQ=255)

                              aCTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATg                                                                                                   >  2:557735/1‑140 (MQ=255)

                              aCTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATg                                                                                                   >  1:299064/1‑140 (MQ=255)

                              aCTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATg                                                                                                   >  1:93526/1‑140 (MQ=255)

                              aCTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTCACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATg                                                                                                   >  1:337715/1‑140 (MQ=255)

                                 cGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATgcgc                                                                                                >  2:186533/1‑140 (MQ=255)

                                     cACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCa                                                                                            <  1:738285/140‑1 (MQ=255)

                                     cACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCa                                                                                            <  1:640710/140‑1 (MQ=255)

                                          tCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACg                                                                                       <  1:557735/140‑1 (MQ=255)

                                              tCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACt                                                                                   >  1:831162/1‑140 (MQ=255)

                                                 tGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTcc                                                                                >  1:435941/1‑140 (MQ=255)

                                                      gCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCAcgcg                                                                           <  2:787863/140‑1 (MQ=255)

                                                      caggTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCAcgcg                                                                           <  1:708937/137‑1 (MQ=255)

                                                       ccGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACgcgc                                                                          <  2:299064/140‑1 (MQ=255)

                                                         gTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGccc                                                                        <  2:23757/140‑1 (MQ=255)

                                                         gTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGccc                                                                        <  1:91926/140‑1 (MQ=255)

                                                           aaTACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTCTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCtt                                                                      <  2:161478/140‑1 (MQ=255)

                                                             tACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCa                                                                    <  1:879732/140‑1 (MQ=255)

                                                                 gTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCAt                                                                >  1:779847/1‑140 (MQ=255)

                                                                  ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATg                                                               >  2:879858/1‑140 (MQ=255)

                                                                  ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATg                                                               >  1:203387/1‑140 (MQ=255)

                                                                  ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATg                                                               >  1:507952/1‑140 (MQ=255)

                                                                  ttCGGGGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATg                                                               >  2:181783/1‑140 (MQ=255)

                                                                    cGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATgag                                                             >  2:540389/1‑140 (MQ=255)

                                                                     ggTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCtt                                                                      <  1:181350/130‑1 (MQ=255)

                                                                     ggTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCtt                                                                      >  2:181350/1‑130 (MQ=255)

                                                                       tGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATgagaga                                                          >  1:270839/1‑140 (MQ=255)

                                                                              ttCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAAt                                                   <  1:439117/140‑1 (MQ=255)

                                                                              ttCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAAt                                                   >  2:106458/1‑140 (MQ=255)

                                                                                        ccGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGc                                         >  1:483498/1‑140 (MQ=255)

                                                                                        ccGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGc                                         <  2:129908/140‑1 (MQ=255)

                                                                                                  ttCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCccacca                               <  2:465879/140‑1 (MQ=255)

                                                                                                             tGCCGAAGTTATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAAc                    >  1:232305/1‑140 (MQ=255)

                                                                                                                     ttATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAgaga            <  1:117601/140‑1 (MQ=255)

                                                                                                                     ttATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAgaga            >  2:406892/1‑140 (MQ=255)

                                                                                                                     ttATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAgaga            <  1:406892/140‑1 (MQ=255)

                                                                                                                     ttATCGACATAGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAgaga            >  2:117601/1‑140 (MQ=255)

                                                                                                                               aGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTgg  >  1:487424/1‑140 (MQ=255)

                                                                                                                               aGCGCAGTGTCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTgg  >  1:153209/1‑140 (MQ=255)

ACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGG  >  NC_000913/2691753‑2692019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A