Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,693,393	A→G	G717G (GGT→GGC)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,693,393	0	A	G	100.0%	152.7 / NA	44	G717G (GGT→GGC)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/23); total (21/23)

CCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCC  >  NC_000913/2693262‑2693526

cccACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGc                                                                                                                               <  1:247716/140‑1 (MQ=255)

 ccACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGcc                                                                                                                              >  2:287710/1‑140 (MQ=255)

  cACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCa                                                                                                                             <  1:86518/140‑1 (MQ=255)

   aCGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAg                                                                                                                            <  2:481692/140‑1 (MQ=255)

              ggCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAgg                                                                                                                 <  1:751343/140‑1 (MQ=255)

                               cATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCagcagc                                                                                                >  1:342915/1‑140 (MQ=255)

                                           ggTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCTGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCa                                                                                    >  2:1029153/1‑140 (MQ=255)

                                           ggTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCa                                                                                    >  2:371795/1‑140 (MQ=255)

                                           ggTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCa                                                                                    <  1:464808/140‑1 (MQ=255)

                                             tGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACg                                                                                  >  1:644938/1‑140 (MQ=255)

                                               gCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGACGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCt                                                                                >  2:1051478/1‑140 (MQ=255)

                                                ccTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTc                                                                               >  1:122242/1‑140 (MQ=255)

                                                     cgccgccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCaa                                                                          >  2:892101/1‑140 (MQ=255)

                                                     cgccgccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCaa                                                                          >  1:437784/1‑140 (MQ=255)

                                                      gccgccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAAt                                                                         >  2:1071865/1‑140 (MQ=255)

                                                         gccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGc                                                                      >  1:1051966/1‑140 (MQ=255)

                                                           cATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCa                                                                    <  2:342915/140‑1 (MQ=255)

                                                               cAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGc                                                                <  1:263128/140‑1 (MQ=255)

                                                                aGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCt                                                               <  2:657405/140‑1 (MQ=255)

                                                                   tGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCa                                                            <  2:480443/140‑1 (MQ=255)

                                                                       ggAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCgt                                                        >  2:487205/1‑140 (MQ=255)

                                                                       ggAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCgt                                                        >  1:9565/1‑140 (MQ=255)

                                                                       ggAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCgt                                                        >  2:871498/1‑140 (MQ=255)

                                                                           aGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCgtagta                                                    <  1:1051478/140‑1 (MQ=255)

                                                                            gTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGtagtag                                                   <  1:287710/140‑1 (MQ=255)

                                                                                      tttGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTGCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGc                                         >  2:1007587/1‑140 (MQ=255)

                                                                                       ttGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCt                                        >  1:352092/1‑140 (MQ=255)

                                                                                        tGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTg                                       <  1:871498/140‑1 (MQ=255)

                                                                                                ccAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAgg                                          <  2:860152/129‑1 (MQ=255)

                                                                                                ccAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAgg                                          >  1:860152/1‑129 (MQ=255)

                                                                                                ccAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGt                               >  2:235818/1‑140 (MQ=255)

                                                                                                   aTTTGTGTTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGAc                            <  2:644938/140‑1 (MQ=255)

                                                                                                       gtgtTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACcgcg                        <  2:352092/140‑1 (MQ=255)

                                                                                                        tgtTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCgcgc                       <  2:437784/140‑1 (MQ=255)

                                                                                                        tgtTGCGGCGATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCgcgc                       >  2:924138/1‑140 (MQ=255)

                                                                                                             cggcgATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTa                  >  2:36894/1‑140 (MQ=255)

                                                                                                             cggcgATGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTa                  >  2:215961/1‑140 (MQ=255)

                                                                                                                  aTGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGAc             <  1:107959/140‑1 (MQ=255)

                                                                                                                  aTGTTGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGAc             <  1:36894/140‑1 (MQ=255)

                                                                                                                     ttGGTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCgg          <  1:1071865/140‑1 (MQ=255)

                                                                                                                       ggTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCa        <  1:144310/140‑1 (MQ=255)

                                                                                                                        gTTAACGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCAc       <  2:122242/140‑1 (MQ=255)

                                                                                                                            aCGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGc   <  2:9565/140‑1 (MQ=255)

                                                                                                                             cGCTTCGCCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGcc  <  1:235818/140‑1 (MQ=255)

CCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCC  >  NC_000913/2693262‑2693526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A