Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,695,843	G→A	A15T (GCA→ACA)	mltF →	membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,695,843	0	G	A	100.0%	91.3 / NA	27	A15T (GCA→ACA)	mltF	membrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/11); total (16/11)

TTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATC  >  NC_000913/2695710‑2695977

ttACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACActgc                                                                                                                                  >  1:632432/1‑140 (MQ=255)

        aaTGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTcgcg                                                                                                                          >  2:441555/1‑140 (MQ=255)

                    ttATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTgg                                                                                                              >  1:414404/1‑140 (MQ=255)

                         aGTAAACCTTTCACTATTATTACGCTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGccatc                                                                                                         >  2:57775/1‑140 (MQ=255)

                            aaaCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGccatccat                                                                                                      >  1:497294/1‑140 (MQ=255)

                            aaaCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGcc                                                                                                            >  2:601594/1‑134 (MQ=255)

                            aaaCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGcc                                                                                                            <  1:601594/134‑1 (MQ=255)

                              aCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGTATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGccat                                                                                                          <  2:860564/134‑1 (MQ=255)

                              aCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGTATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGccat                                                                                                          >  1:860564/1‑134 (MQ=255)

                              aCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTc                                                                                                    >  2:152377/1‑140 (MQ=255)

                                                   ttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACa                                                                               >  1:734271/1‑140 (MQ=255)

                                                   ttttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAACTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACa                                                                               >  1:79326/1‑140 (MQ=255)

                                                              ggACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATcgcc                                                                    <  1:152377/140‑1 (MQ=255)

                                                                               agaATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCgggg                                                   <  2:153913/140‑1 (MQ=255)

                                                                                    tAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAgcgc                                                       <  1:92998/131‑1 (MQ=255)

                                                                                    tAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAgcgc                                                       >  2:92998/1‑131 (MQ=255)

                                                                                    tAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGt                                              >  1:90390/1‑140 (MQ=255)

                                                                                              aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAg                                    <  1:57775/140‑1 (MQ=255)

                                                                                              aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAg                                    <  1:38343/140‑1 (MQ=255)

                                                                                               aaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGc                                   <  1:820983/140‑1 (MQ=255)

                                                                                                 aaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCAc                                 <  2:414404/140‑1 (MQ=255)

                                                                                                         gATTAATTATCTGTTCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCAACCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATa                         <  2:632432/140‑1 (MQ=255)

                                                                                                                      ttCATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTta            >  2:225994/1‑140 (MQ=255)

                                                                                                                        cATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGAc               >  2:204377/1‑135 (MQ=255)

                                                                                                                        cATCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGAc               <  1:204377/135‑1 (MQ=255)

                                                                                                                         aTCGGCATTCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATGCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCAGACTCCCCTGACTTATaa         >  2:795273/1‑140 (MQ=255)

                                                                                                                                ttCTGACACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATc  >  1:480272/1‑140 (MQ=255)

TTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATC  >  NC_000913/2695710‑2695977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A