Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,698,929	(A)_5→6	coding (171/849 nt)	yfhH →	putative DNA‑binding transcriptional regulator YfhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,698,924	1	.	A	100.0%	154.2 / NA	44	Q56K (CAA→AAA)	yfhH	putative DNA‑binding transcriptional regulator YfhH
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (22/22); total (22/22)

TACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGC‑AAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGT  >  NC_000913/2698789‑2699051

tACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGC‑aaaa                                                                                                                             <  1:777738/140‑1 (MQ=255)

  ccAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGC‑aaaaaa                                                                                                                           <  2:511590/140‑2 (MQ=255)

     ggggCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGC‑aaaaaactc                                                                                                                        <  1:398775/140‑5 (MQ=255)

            gCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCtata                                                                                                                  >  2:582638/1‑140 (MQ=255)

                     gATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTc                                                                                                         <  2:541819/140‑1 (MQ=255)

                      aTAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTcc                                                                                                        >  2:380535/1‑140 (MQ=255)

                          aaaaCTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGgcg                                                                                                    <  1:536391/140‑1 (MQ=255)

                                                ctACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAg                                                                              >  1:37272/1‑140 (MQ=255)

                                                ctACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAg                                                                              >  2:53994/1‑140 (MQ=255)

                                                          aTACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAag                                                                    >  1:50142/1‑140 (MQ=255)

                                                          aTACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAag                                                                    >  2:607083/1‑140 (MQ=255)

                                                          aTACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAag                                                                    >  2:743788/1‑140 (MQ=255)

                                                          aTACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAag                                                                    <  1:793070/140‑1 (MQ=255)

                                                                cgcgcCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAagccagcc                                                              >  2:3387/1‑140 (MQ=255)

                                                                cgcgcCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAagccagcc                                                              >  2:81831/1‑140 (MQ=255)

                                                                 gcgcCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCg                                                             <  2:469256/140‑1 (MQ=255)

                                                                 gcgcCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCg                                                             <  2:37272/140‑1 (MQ=255)

                                                                  cgcCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCgg                                                            >  2:125808/1‑140 (MQ=255)

                                                                         tAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCAcc                                                     <  1:125808/140‑1 (MQ=255)

                                                                               ctcAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGt                                               >  2:844306/1‑140 (MQ=255)

                                                                               ctcAGCAACTGGCCAACGAAGCCGGAGACAGTCAGTCCAGCGTCGCGAAG‑TTCGCGCAAAAAAATCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTCGCAAGCCACCCGGCATCACCCTCCGt                                               >  2:726990/1‑140 (MQ=255)

                                                                                 cAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAagccagc                                                               >  1:826026/1‑122 (MQ=255)

                                                                                 cAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAagccagc                                                               <  2:826026/122‑1 (MQ=255)

                                                                                 cAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGc                                             <  2:120478/140‑1 (MQ=255)

                                                                                 cAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGc                                             >  1:120478/1‑140 (MQ=255)

                                                                                  aGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGcc                                            <  1:844306/140‑1 (MQ=255)

                                                                                  aGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGcc                                            <  1:724738/140‑1 (MQ=255)

                                                                                  aGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGcc                                            >  2:768456/1‑140 (MQ=255)

                                                                                  aGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCAGAATCACCCTCCGTGcc                                            <  1:607083/140‑1 (MQ=255)

                                                                                         ggCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTc                                       >  1:229183/1‑138 (MQ=255)

                                                                                         ggCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTc                                       <  2:229183/138‑1 (MQ=255)

                                                                                          gCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTc                                       <  2:500538/137‑1 (MQ=255)

                                                                                          gCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTc                                       >  1:500538/1‑137 (MQ=255)

                                                                                          gCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATa                                    >  1:724251/1‑140 (MQ=255)

                                                                                               cGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCaa                               <  1:582638/140‑1 (MQ=255)

                                                                                                  aGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATc                            >  1:196014/1‑140 (MQ=255)

                                                                                                  aGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATc                            <  2:724251/140‑1 (MQ=255)

                                                                                                    ctggaagcagtcagtcCAAGGGCGTGAAGTTTCGCCA‑AAAAACCCGGGTATAAAAGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCcg                          <  2:817788/133‑1 (MQ=255)

                                                                                                    ccGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCcg                          <  1:81831/140‑1 (MQ=255)

                                                                                                    ccGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCcg                          <  1:743788/140‑1 (MQ=255)

                                                                                                    ccGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCcg                          >  1:751082/1‑140 (MQ=255)

                                                                                                    ccGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCcg                          <  1:768456/140‑1 (MQ=255)

                                                                                                     cGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCcgc                         <  1:53994/140‑1 (MQ=255)

                                                                                                             gtcagtcCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCCGCGGtgatga                 <  2:659072/140‑1 (MQ=255)

                                                                                                             gtcagtcCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCCGCGGtgatga                 <  2:50142/140‑1 (MQ=255)

                                                                                                             gtcagtcCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCAGGAATCACCATCCGTGCCCATTCATAACCAAATCCGCGGtgatga                 <  2:607757/140‑1 (MQ=255)

                                                                                                              tcagtcCAGCGTCGTGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCCGCGGTgatgat                >  1:398172/1‑140 (MQ=255)

                                                                                                                            tGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGt  >  2:517165/1‑140 (MQ=255)

                                                                                                                            tGAAG‑TTCGCGCAAAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGt  >  2:466792/1‑140 (MQ=255)

TACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAG‑TTCGCGC‑AAAAACTCGGCTATAAAGGTTTTCCGGCGCTTAAGTTGGCGTTGAGTGAAGCGCTGGCAAGCCAGCCGGAATCACCCTCCGTGCCCATTCATAACCAAATCCGCGGTGATGATCCGTTACGGCTGGT  >  NC_000913/2698789‑2699051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A