Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,699,776 T→C C38C (TGT→TGC)  yfhL → putative 4Fe‑4S cluster‑containing protein YfhL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,699,7760TC100.0% 215.3 / NA 61C38C (TGT→TGC) yfhLputative 4Fe‑4S cluster‑containing protein YfhL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/25);  total (36/25)

GTGTTGTTTTGAGAGTTTCCTTATGGCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCAC  >  NC_000913/2699641‑2699908
                                                                                                                                       |                                                                                                                                    
gtgtTGTTTTGAGAGTTTCCTTATGGCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCg                                                                                                                                  <  2:709950/140‑1 (MQ=255)
 tgttgtTTTGAGAGTTTCCTTATGGCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGa                                                                                                                                 <  1:748957/140‑1 (MQ=255)
 tgttgtTTTGAGAGTTTCCTTATGGCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGa                                                                                                                                 <  2:279848/140‑1 (MQ=255)
      ttttGAGAGTTTCCTTATGGCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCg                                                                                                                            <  1:465559/140‑1 (MQ=255)
           agagTTTCCTTATGGCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGa                                                                                                                                 >  1:686581/1‑130 (MQ=255)
           agagTTTCCTTATGGCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGa                                                                                                                                 <  2:686581/130‑1 (MQ=255)
                ttCCTTATGGCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCg                                                                                                                            >  1:697792/1‑130 (MQ=255)
                ttCCTTATGGCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCg                                                                                                                            <  2:697792/130‑1 (MQ=255)
                         gCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACAcc                                                                                                         >  1:472826/1‑140 (MQ=255)
                           gttgttAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCaa                                                                                                       <  2:234570/140‑1 (MQ=255)
                            ttgttAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGGCACCAAc                                                                                                      >  2:736097/1‑140 (MQ=255)
                            ttgttAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACAcc                                                                                                         >  1:34640/1‑137 (MQ=255)
                            ttgttAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACAcc                                                                                                         <  2:34640/137‑1 (MQ=255)
                            ttgttAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAAc                                                                                                      >  1:824481/1‑140 (MQ=255)
                            ttgttAATCACTAAAAAATGCATCAACTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAAc                                                                                                      <  1:620918/140‑1 (MQ=255)
                               ttAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTg                                                                                                   >  1:789544/1‑140 (MQ=255)
                                       tAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAgg                                                                                           >  1:699930/1‑140 (MQ=255)
                                         aaaaaTGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGgtg                                                                                         >  1:541179/1‑140 (MQ=255)
                                              tGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGccc                                                                                    <  2:509369/140‑1 (MQ=255)
                                               gCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACATCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCg                                                                                   <  2:789544/140‑1 (MQ=255)
                                                 aTCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCa                                                                                                >  1:660064/1‑125 (MQ=255)
                                                 aTCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCa                                                                                                <  2:660064/125‑1 (MQ=255)
                                                 aTCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAg                                                                                               >  1:774843/1‑126 (MQ=255)
                                                 aTCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAg                                                                                               <  2:774843/126‑1 (MQ=255)
                                                 aTCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAACGCGTAGGGCACTACGAGACACCAACCTGCCAg                                                                                               <  2:404734/126‑1 (MQ=255)
                                                 aTCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAACGCGTAGGGCACTACGAGACACCAACCTGCCAg                                                                                               >  1:404734/1‑126 (MQ=255)
                                                  tCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATc                                                                                <  2:699930/140‑1 (MQ=255)
                                                    aaTTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATccc                                                                              <  2:508891/140‑1 (MQ=255)
                                                    aaTTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATccc                                                                              <  2:142894/140‑1 (MQ=255)
                                                         tgATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCg                                                                                   >  1:115177/1‑130 (MQ=255)
                                                         tgATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCg                                                                                   <  2:115177/130‑1 (MQ=255)
                                                           atatGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACt                                                                       <  2:141630/140‑1 (MQ=255)
                                                              tgtgtgAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTAtt                                                                    <  1:123191/140‑1 (MQ=255)
                                                                  tgAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGa                                                                >  2:200224/1‑140 (MQ=255)
                                                                  tgAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGa                                                                >  1:79159/1‑140 (MQ=255)
                                                                   gAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGaa                                                               >  1:688782/1‑140 (MQ=255)
                                                                     aCCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTAAGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAg                                                             >  2:824388/1‑140 (MQ=255)
                                                                        cgaatgcccgaatgAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATc                                                          >  2:466026/1‑140 (MQ=255)
                                                                         gaatgcccgaatgAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATcc                                                         >  1:82804/1‑140 (MQ=255)
                                                                          aatgcccgaatgAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCg                                                        >  2:256578/1‑140 (MQ=255)
                                                                                             ttCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACagaag                                     >  1:719924/1‑140 (MQ=255)
                                                                                                      agaTCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCgaga                                           <  1:763937/125‑1 (MQ=255)
                                                                                                      agaTCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCgaga                                           >  2:763937/1‑125 (MQ=255)
                                                                                                       gaTCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTtgtg                           >  1:589782/1‑140 (MQ=255)
                                                                                                        aTCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTgg                          >  1:762690/1‑140 (MQ=255)
                                                                                                        aTCATATCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATATCGAGACAGAAGAACAGTTGTgg                          >  2:247720/1‑140 (MQ=255)
                                                                                                              tCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGa                        >  2:14889/1‑136 (MQ=255)
                                                                                                              tCTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGa                        <  1:14889/136‑1 (MQ=255)
                                                                                                               cTACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAt                   >  1:465745/1‑140 (MQ=255)
                                                                                                                tACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAtt                  >  2:634408/1‑140 (MQ=255)
                                                                                                                tACGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAtt                  >  1:538442/1‑140 (MQ=255)
                                                                                                                  cGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTtg                >  2:12293/1‑140 (MQ=255)
                                                                                                                  cGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTACCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTtg                >  1:760042/1‑140 (MQ=255)
                                                                                                                  cGAGATTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTACCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTtg                <  2:760042/140‑1 (MQ=255)
                                                                                                                    agaTTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTtgtg              <  1:256578/140‑1 (MQ=255)
                                                                                                                      aTTAACAGCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCt            >  2:324943/1‑140 (MQ=255)
                                                                                                                             gCGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcac     <  1:200224/140‑1 (MQ=255)
                                                                                                                              cGATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcacc    >  2:439417/1‑140 (MQ=255)
                                                                                                                               gATAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcacca   >  2:498604/1‑140 (MQ=255)
                                                                                                                                aTAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcaccac  >  2:378480/1‑140 (MQ=255)
                                                                                                                                aTAAGTGCACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcaccac  >  1:168998/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GTGTTGTTTTGAGAGTTTCCTTATGGCGTTGTTAATCACTAAAAAATGCATCAATTGTGATATGTGTGAACCCGAATGCCCGAATGAGGCGATTTCAATGGGAGATCATATCTACGAGATTAACAGCGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCAC  >  NC_000913/2699641‑2699908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A