Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,705,644	G→A	A494V (GCG→GTG)	lepA ←	elongation factor 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,705,644	0	G	A	100.0%	148.0 / NA	42	A494V (GCG→GTG)	lepA	elongation factor 4
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/20); total (22/20)

ATCGCGCAATGATGTGCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCAC  >  NC_000913/2705507‑2705777

aTCGCGCAATGATGTGCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGcaca                                                                                                                                     <  2:848526/140‑1 (MQ=255)

aTCGCGCAATGATGTGCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGcaca                                                                                                                                     >  2:126042/1‑140 (MQ=255)

    cgcAATGATGTGCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCaa                                                                                                                                 >  1:579226/1‑140 (MQ=255)

     gcAATGATGTGCGTACCAATCGCTGCCTGAATGGCGATTTCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAac                                                                                                                                <  2:868487/140‑1 (MQ=255)

     gcAATGATGTGCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAac                                                                                                                                <  2:868486/140‑1 (MQ=255)

            tgtgCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCa                                                                                                                         <  1:626382/140‑1 (MQ=255)

               gCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCg                                                                                                                      <  2:395896/140‑1 (MQ=255)

                cGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGt                                                                                                                     <  1:31925/140‑1 (MQ=255)

                cGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGt                                                                                                                     <  1:448567/140‑1 (MQ=255)

                  tACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTg                                                                                                                   <  1:293500/140‑1 (MQ=255)

                   aCCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGa                                                                                                                  >  1:400390/1‑140 (MQ=255)

                   aCCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGa                                                                                                                  >  2:211302/1‑140 (MQ=255)

                   aCCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGa                                                                                                                  >  2:704099/1‑140 (MQ=255)

                     cAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGAtt                                                                                                                >  2:594990/1‑140 (MQ=255)

                       aTCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTaa                                                                                                              <  1:861965/140‑1 (MQ=255)

                       aTCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTaa                                                                                                              <  2:830113/140‑1 (MQ=255)

                        tCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAAt                                                                                                             <  1:565031/140‑1 (MQ=255)

                            tGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGt                                                                                                         <  1:211302/140‑1 (MQ=255)

                                     ggCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTa                                                                                                <  1:68794/140‑1 (MQ=255)

                                        gATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCa                                                                                             >  1:465061/1‑140 (MQ=255)

                                                    ctgGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCt                                                                                 >  2:440858/1‑140 (MQ=255)

                                                                  gATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCGTGTCGGACGCCTGGAAGCGCTTGAAg                                                                   <  2:579226/140‑1 (MQ=255)

                                                                     cTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTg                                                                <  1:594990/140‑1 (MQ=255)

                                                                       ttCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTa                                                              >  2:15453/1‑140 (MQ=255)

                                                                        tCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGa                                                                              <  2:257257/123‑1 (MQ=255)

                                                                        tCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGa                                                                              >  1:257257/1‑123 (MQ=255)

                                                                        tCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTaa                                                             >  2:495308/1‑140 (MQ=255)

                                                                           tcttctCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATcc                                                          >  1:465833/1‑140 (MQ=255)

                                                                             ttctCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCag                                                        <  2:164855/140‑1 (MQ=255)

                                                                               ctcCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCagag                                                      >  2:209738/1‑140 (MQ=255)

                                                                                      aaCTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATa                                               >  2:259794/1‑140 (MQ=255)

                                                                                          cgcgACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCa                                           >  2:853644/1‑140 (MQ=255)

                                                                                            cgACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTGCCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCAcg                                         <  1:259794/140‑1 (MQ=255)

                                                                                             gACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCAcgc                                        <  2:400390/140‑1 (MQ=255)

                                                                                                          cGAATTATCACGGTGGGTGATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCa                           <  1:440858/140‑1 (MQ=255)

                                                                                                                         ggtgATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGTGGTAGATTTCAGGCGATCGaagaagg            >  2:28307/1‑139 (MQ=255)

                                                                                                                         ggtgATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGt            >  2:634301/1‑140 (MQ=255)

                                                                                                                         ggtgATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGt            >  2:114087/1‑140 (MQ=255)

                                                                                                                         ggtgATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGt            >  1:659108/1‑140 (MQ=255)

                                                                                                                           tgATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCg          >  2:176022/1‑140 (MQ=255)

                                                                                                                           tgATCAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCg          >  1:152714/1‑140 (MQ=255)

                                                                                                                               cAACGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGca      <  1:209738/140‑1 (MQ=255)

                                                                                                                                 aCGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTATTCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcacc    >  2:504033/1‑140 (MQ=255)

                                                                                                                                 aCGCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcacc    >  1:18998/1‑140 (MQ=255)

                                                                                                                                   gCCAGCACATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcaccac  >  1:314697/1‑140 (MQ=255)

ATCGCGCAATGATGTGCGTACCAATCGCTGCCTGAATGGCGATATCAAACTGCTGGCGTGGGATCAGATCTTTCATCTTCTCCACCAACTCGCGACCGCGGTTTTGCGAATTATCACGGTGGGTGATCAACGCCAGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCAC  >  NC_000913/2705507‑2705777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A