Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,707,690	G→A	R38C (CGC→TGC)	rseC ←	protein RseC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,707,690	0	G	A	100.0%	198.5 / NA	56	R38C (CGC→TGC)	rseC	protein RseC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/27); total (29/27)

GTAATGCGGAGCTAAGCAGGCTGCCTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATT  >  NC_000913/2707564‑2707824

gTAATGCGGAGCTAAGCAGGCTGCCTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCg                                                                                                                           >  2:466207/1‑140 (MQ=255)

  aaTGCGGAGCTAAGCAGGCTGCCTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGgc                                                                                                                         >  1:403785/1‑140 (MQ=255)

   aTGCGGAGCTAAGCAGGCTGCCTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGgcg                                                                                                                        >  1:53956/1‑140 (MQ=255)

    tGCGGAGCTAAGCAGGCTGCCTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGgcgc                                                                                                                       >  1:105346/1‑140 (MQ=255)

         aGCTAAGCAGGCTGCCTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGaa                                                                                                                  >  2:737824/1‑140 (MQ=255)

            tAAGCAGGCTGCCTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAgcg                                                                                                               <  1:445964/140‑1 (MQ=255)

              aGCAGGCTGCCTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCa                                                                                                             >  2:419073/1‑140 (MQ=255)

               gCAGGCTGCCTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAg                                                                                                            <  2:53956/140‑1 (MQ=255)

                       ccTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCa                                                                                                    >  1:296115/1‑140 (MQ=255)

                         ttCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATg                                                                                                  <  2:403785/140‑1 (MQ=255)

                                          ccACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACaa                                                                                 <  2:170275/140‑1 (MQ=255)

                                             cTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACAt                                                                                      >  1:264786/1‑132 (MQ=255)

                                             cTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACAt                                                                                      <  2:264786/132‑1 (MQ=255)

                                             cTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTg                                                                              >  1:805763/1‑140 (MQ=255)

                                               ttttGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGAc                                                                            <  1:382110/140‑1 (MQ=255)

                                                                  gCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGc                                                         <  2:105346/140‑1 (MQ=255)

                                                                   cTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGcc                                                        >  1:364906/1‑140 (MQ=255)

                                                                   cTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGcc                                                        >  1:254964/1‑140 (MQ=255)

                                                                    tcatcaCAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCa                                                       >  1:188066/1‑140 (MQ=255)

                                                                    tcatcaCAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCa                                                       <  1:220072/140‑1 (MQ=255)

                                                                     catcaCAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAg                                                      <  2:517997/140‑1 (MQ=255)

                                                                         acaGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGgaga                                                  <  1:419073/140‑1 (MQ=255)

                                                                              gTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGacgacg                                             <  2:628867/140‑1 (MQ=255)

                                                                                aCGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGt                                           <  1:737824/140‑1 (MQ=255)

                                                                                     aaTGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGccc                                      <  1:665900/140‑1 (MQ=255)

                                                                                       tGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCAc                                    >  2:780203/1‑140 (MQ=255)

                                                                                       tGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCAc                                    >  2:344816/1‑140 (MQ=255)

                                                                                         gTATGTGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCCACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGccc                                      <  2:790792/136‑1 (MQ=255)

                                                                                         gTATGTGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGccc                                      >  1:790792/1‑136 (MQ=255)

                                                                                         gTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACACCACAACTGACCAGCGCCTGCCCGTTTTGCTAGGAGACGACGGTAGCCCActc                                  >  1:774988/1‑140 (MQ=255)

                                                                                             gCGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTg                              >  1:471143/1‑140 (MQ=255)

                                                                                              cGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGccc                                      <  1:289846/131‑1 (MQ=255)

                                                                                              cGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGccc                                      >  2:289846/1‑131 (MQ=255)

                                                                                              cGTGGTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGa                             >  1:766984/1‑140 (MQ=255)

                                                                                                 ggTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCa                          <  1:615163/140‑1 (MQ=255)

                                                                                                 ggTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCa                          >  1:632791/1‑140 (MQ=255)

                                                                                                 ggTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCa                          >  1:359156/1‑140 (MQ=255)

                                                                                                 ggTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCa                          <  2:364906/140‑1 (MQ=255)

                                                                                                  gTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGccc                                      <  1:709484/127‑1 (MQ=255)

                                                                                                  gTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGccc                                      >  2:709484/1‑127 (MQ=255)

                                                                                                  gTTTGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCAt                         >  2:714502/1‑140 (MQ=255)

                                                                                                   tttGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCAc                                    <  2:82579/128‑1 (MQ=255)

                                                                                                   tttGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCAc                                    >  1:82579/1‑128 (MQ=255)

                                                                                                   tttGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCAtt                        >  2:371177/1‑140 (MQ=255)

                                                                                                     tGCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTgc                      >  2:697672/1‑140 (MQ=255)

                                                                                                      gCGGGCCAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTgcg                     <  2:632791/140‑1 (MQ=255)

                                                                                                           ccAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGcc                <  1:466207/140‑1 (MQ=255)

                                                                                                            cAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGccc               <  2:254964/140‑1 (MQ=255)

                                                                                                            cAAGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGccc               <  2:766984/140‑1 (MQ=255)

                                                                                                             aaGTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGcccc              >  2:3479/1‑140 (MQ=255)

                                                                                                               gTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGccccaa            <  2:479203/140‑3 (MQ=255)

                                                                                                               gTTTATTTAACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGa            <  1:344816/140‑1 (MQ=255)

                                                                                                                    tttaACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTa       <  2:471143/140‑1 (MQ=255)

                                                                                                                    tttaACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTa       <  1:755052/140‑1 (MQ=255)

                                                                                                                    tttaACACGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTa       >  1:713422/1‑140 (MQ=255)

                                                                                                                         cacGCAGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATAtt  >  2:109566/1‑140 (MQ=255)

GTAATGCGGAGCTAAGCAGGCTGCCTTCGGCGATCCCCAATTCCACTTTTTGCCCCGGCACTAACGGCTCATCACAGGGTACGACAATGGTATGCGTGGTTTGCGGGCCAAGTTTATTTAACACGCGGCTACCGCAACCGGCGCGTGAAGCGCAGCTGCTGCATGAAGCTTTAACATCACAACTGACCAGCGCCTGCCCGTTTTGCCAGGAGACGACGGTAGCCCACTCTTTGATCATTGCGCTGCCCCGAACTTAATATT  >  NC_000913/2707564‑2707824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A