Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
163 |
52.9 |
1786516 |
99.3% |
1774010 |
139.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,824,067 |
C→T |
A94T (GCT→ACT) |
pncC ← |
NMN aminohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,824,067 | 0 | C | T | 98.2%
| 190.6
/ NA
| 56 | A94T (GCT→ACT) | pncC | NMN aminohydrolase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (25/30); total (26/30) |
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTAGGTGACAA > NC_000913/2823938‑2824189
|
aTTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGt < 2:804688/140‑1 (MQ=255)
ttCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTg > 1:826949/1‑140 (MQ=255)
gccgGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTg > 1:181038/1‑135 (MQ=255)
gccgGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTg < 2:181038/135‑1 (MQ=255)
ggTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCATTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCg < 2:513578/140‑1 (MQ=255)
ggTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCg < 2:588637/140‑1 (MQ=255)
tGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCAtt < 2:77852/140‑1 (MQ=255)
tCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTccac > 2:757405/1‑140 (MQ=255)
tCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTccac > 2:743087/1‑140 (MQ=255)
cACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTccacc > 1:346088/1‑140 (MQ=255)
cACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTccacc > 1:496531/1‑140 (MQ=255)
gcgGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCacga < 1:646781/140‑1 (MQ=255)
gcgGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCacga < 1:494052/140‑1 (MQ=255)
cgGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCacgac > 2:857058/1‑140 (MQ=255)
cgGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCacgac > 2:735537/1‑140 (MQ=255)
ggCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCacga > 2:335717/1‑137 (MQ=255)
ggCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCacga < 1:335717/137‑1 (MQ=255)
tGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTtca > 1:720961/1‑140 (MQ=255)
aaaaGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTtcactca < 1:735537/140‑1 (MQ=255)
aaaaGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTtcactca < 1:757405/140‑1 (MQ=255)
aaaaGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTtcactca < 2:496531/140‑1 (MQ=255)
aaaaGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTtcactca < 2:49556/140‑1 (MQ=255)
aaGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACggg < 1:768003/130‑1 (MQ=255)
aaGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACggg > 2:768003/1‑130 (MQ=255)
aaGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCAcc > 1:252901/1‑140 (MQ=255)
aCCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCa > 2:279813/1‑140 (MQ=255)
aCAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGt > 1:27506/1‑140 (MQ=255)
tctcTTCACTACCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTcg > 1:181847/1‑140 (MQ=255)
tctTCACTGCCGCCATCCGGCCCGGCGATACCGCTAATAGACTCGGCATAATCAGCGCGAGTCGCTTTCAGTGCGCCTGTCGCCGTTTCCTCCTCGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTcgcg < 2:556364/140‑1 (MQ=255)
tctTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTcgcg < 2:124505/140‑1 (MQ=255)
ttCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTa < 1:770291/140‑1 (MQ=255)
tGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCg < 1:411221/140‑1 (MQ=255)
tGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCg > 2:352843/1‑140 (MQ=255)
ccgccATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTGCACCACGACGGGTTCACTCACCGCGCCATGCTGAGCCAGCGTCTCTTCGCGTACGCCGat > 2:472068/1‑140 (MQ=255)
ccgccATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGat > 2:493235/1‑140 (MQ=255)
ccgccATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGat < 1:493235/140‑1 (MQ=255)
tCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTg < 2:756604/140‑1 (MQ=255)
gcccggcGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCt < 1:762283/140‑1 (MQ=255)
ggcGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTgg < 1:102993/140‑1 (MQ=255)
gcGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCTTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGc < 1:259093/140‑1 (MQ=255)
gcGATACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGc < 2:27506/140‑1 (MQ=255)
aTACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTc < 2:346088/140‑1 (MQ=255)
aTACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTc < 2:686188/140‑1 (MQ=255)
tACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCg < 2:505081/140‑1 (MQ=255)
tACCACTAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCg < 1:857058/140‑1 (MQ=255)
tAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTg < 1:510049/140‑1 (MQ=255)
tAATAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTg < 1:352843/140‑1 (MQ=255)
aTAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTa < 2:181847/140‑1 (MQ=255)
aTAGACACGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTa < 1:63908/140‑1 (MQ=255)
acacGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTAGGTg > 1:323559/1‑140 (MQ=255)
acacGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTAGGTg > 1:269540/1‑140 (MQ=255)
cacGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTAGGTGa > 2:187568/1‑140 (MQ=255)
acGGCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTAGGTGAc > 1:587708/1‑140 (MQ=255)
ggCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTAGGTGACaa > 2:120901/1‑140 (MQ=255)
ggCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTAGGTGACaa > 2:521037/1‑140 (MQ=255)
ggCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTAGGTGACaa > 2:824695/1‑140 (MQ=255)
ggCATAATCAGCACGAGTCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTAGGTGACaa > 2:509228/1‑140 (MQ=255)
|
ATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCACGAGCCGCTTTCAGTGCGCCTATCGCCATTTCCACCACGACGGGTTCACTCACCGCGCCATGCTGCGCCAGCGTCTCTTCGCGTACGCCGATCATCTGCGCTTTGGCTTCGTTACTGTAGGTGACAA > NC_000913/2823938‑2824189
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
