Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,824,478	(C)_6→7	intergenic (‑132/+13)	pncC ← / ← mltB	NMN aminohydrolase/membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,824,472	1	.	C	98.9%	333.7 / ‑3.8	90	intergenic (‑126/+19)	pncC/mltB	NMN aminohydrolase/membrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (37/52); total (37/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 116 total aligned reads displayed.

ACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑C‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGT  >  NC_000913/2824335‑2824603

aCTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑c                                                                                                                                   <  1:859935/140‑1 (MQ=255)

  tGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccc                                                                                                                                 >  2:673859/1‑140 (MQ=255)

  tGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccc                                                                                                                                 <  2:565022/140‑1 (MQ=255)

  tGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccc                                                                                                                                 <  2:837109/140‑1 (MQ=255)

  tGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccc                                                                                                                                 >  1:646003/1‑140 (MQ=255)

   gtcagtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑cccc                                                                                                                                <  1:650141/140‑1 (MQ=255)

    tcagtcaTCACAACCTCTGTCAGTCAAAAATTTATGTCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccccc                                                                                                                               <  1:270566/140‑1 (MQ=255)

    tcagtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ctccc                                                                                                                               <  2:1120779/140‑1 (MQ=255)

    tcagtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccccc                                                                                                                               <  1:897598/140‑1 (MQ=255)

    tcagtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccccc                                                                                                                               <  2:1232288/140‑1 (MQ=255)

    tcagtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccccc                                                                                                                               <  2:36172/140‑1 (MQ=255)

    tcagtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccccc                                                                                                                               <  2:42358/140‑1 (MQ=255)

    tcagtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTACAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccccc                                                                                                                               <  2:1555231/140‑1 (MQ=255)

     cagtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑cccccc                                                                                                                              <  1:1325490/140‑2 (MQ=255)

      agtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑cccccct                                                                                                                             <  2:1215216/140‑3 (MQ=255)

       gtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑cccgcctc                                                                                                                            >  2:1574734/1‑135 (MQ=255)

       gtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑cccccctc                                                                                                                            >  2:1150371/1‑137 (MQ=255)

       gtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑cccccctc                                                                                                                            >  2:1249257/1‑137 (MQ=255)

       gtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccccc                                                                                                                               >  2:1096587/1‑137 (MQ=255)

       gtcaTCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccccc                                                                                                                               <  1:1096587/137‑1 (MQ=255)

        tcatcaCAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑cccccctct                                                                                                                           >  2:802829/1‑136 (MQ=255)

          atcaCAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTg                                                                                                                          <  1:1272231/140‑1 (MQ=255)

           tcaCAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGt                                                                                                                         >  2:538703/1‑140 (MQ=255)

            cacaACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTa                                                                                                                        >  2:409492/1‑140 (MQ=255)

            cacaACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTa                                                                                                                        <  2:202302/140‑1 (MQ=255)

            cacaACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTa                                                                                                                        >  2:785673/1‑140 (MQ=255)

              caACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTaaa                                                                                                                      >  1:1268798/1‑140 (MQ=255)

                aCCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATa                                                                                                                    <  2:400312/140‑1 (MQ=255)

                aCCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATa                                                                                                                    >  2:1396192/1‑140 (MQ=255)

                 ccTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTg                                                                                                                          <  1:340274/133‑1 (MQ=255)

                 ccTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTg                                                                                                                          >  2:340274/1‑133 (MQ=255)

                  ctctGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGc                                                                                                                  <  2:646003/140‑1 (MQ=255)

                      gtcagtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGc                                                                                                                  <  2:881082/136‑1 (MQ=255)

                      gtcagtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACt                                                                                                              >  1:1249768/1‑140 (MQ=255)

                      gtcagtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑c‑ccccc                                                                                                                               >  1:937123/1‑122 (MQ=255)

                           tcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTg                                                                                                                          >  2:1361100/1‑123 (MQ=255)

                           tcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTg                                                                                                                          <  1:1361100/123‑1 (MQ=255)

                           tcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACt                                                                                                         >  2:1499966/1‑140 (MQ=255)

                            caAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACt                                                                                                              >  1:1020714/1‑134 (MQ=255)

                                aaTTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCgcgc                                                                                                    <  2:4424/140‑1 (MQ=255)

                                 aTTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCgcgcc                                                                                                   <  2:1067202/140‑1 (MQ=255)

                                                   aGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCgcgccagcgcc                                                                                             <  1:1510688/128‑1 (MQ=255)

                                                   aGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCgcgccagcgcc                                                                                             >  2:1510688/1‑128 (MQ=255)

                                                   aGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCt                                                                                 >  2:884390/1‑140 (MQ=255)

                                                   aGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCt                                                                                 <  1:884390/140‑1 (MQ=255)

                                                   aGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCt                                                                                 >  1:1419471/1‑140 (MQ=255)

                                                   aGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCt                                                                                 <  1:628981/140‑1 (MQ=255)

                                                     cACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTaa                                                                               >  1:576095/1‑140 (MQ=255)

                                                          tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGc                                                                                        >  1:730353/1‑126 (MQ=255)

                                                          tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                          >  1:1628207/1‑140 (MQ=255)

                                                          tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                          >  1:1677199/1‑140 (MQ=255)

                                                          tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                          >  2:1670459/1‑140 (MQ=255)

                                                          tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                          >  1:187456/1‑140 (MQ=255)

                                                          tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                          >  2:1145334/1‑140 (MQ=255)

                                                              cAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                      <  1:785673/140‑1 (MQ=255)

                                                              cAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑CACCCCCTCTTGTAAATAGCCACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                      <  2:1525464/140‑1 (MQ=255)

                                                                ggAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCg                                                                    <  1:1141217/140‑1 (MQ=255)

                                                                ggAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCg                                                                    <  1:802829/140‑1 (MQ=255)

                                                                  aaGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGcc                                                                  <  2:740278/140‑1 (MQ=255)

                                                                  aaGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGcc                                                                  <  1:1150371/140‑1 (MQ=255)

                                                                   aGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCa                                                                 <  1:940393/140‑1 (MQ=255)

                                                                    gATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCAt                                                                <  1:998760/140‑1 (MQ=255)

                                                                     aTGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCAtt                                                               <  2:187456/140‑1 (MQ=255)

                                                                           ttGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTaa                                                         >  1:1669537/1‑140 (MQ=255)

                                                                           ttGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTaa                                                         >  1:1328106/1‑140 (MQ=255)

                                                                            tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAAt                                                        >  1:362729/1‑140 (MQ=255)

                                                                            tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAAt                                                        <  2:1628207/140‑1 (MQ=255)

                                                                                gTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGt                                                    <  1:801977/140‑1 (MQ=255)

                                                                                  tGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGAATAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGc                                                  <  2:508192/140‑1 (MQ=255)

                                                                                   gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                 >  2:493819/1‑140 (MQ=255)

                                                                                   gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                 >  1:182208/1‑140 (MQ=255)

                                                                                      aTTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTATTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtgtg                                              <  2:642096/140‑1 (MQ=255)

                                                                                       ttttACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTgg                                             <  1:1499966/140‑1 (MQ=255)

                                                                                            cGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTa                                        <  2:1658784/140‑1 (MQ=255)

                                                                                            cGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTa                                        <  1:673859/140‑1 (MQ=255)

                                                                                               ggaggaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACg                                     <  2:1677199/140‑1 (MQ=255)

                                                                                                  ggaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGt                                  <  2:1669537/140‑1 (MQ=255)

                                                                                                   gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTg                                 <  2:1057/140‑1 (MQ=255)

                                                                                                    aTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                <  1:1145334/140‑1 (MQ=255)

                                                                                                    aTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                <  2:1268798/140‑1 (MQ=255)

                                                                                                    aTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                <  2:576095/140‑1 (MQ=255)

                                                                                                    aTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                <  1:937576/140‑1 (MQ=255)

                                                                                                      tCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATg                              <  2:182208/140‑1 (MQ=255)

                                                                                                          aaaaaaGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGgtgt                          >  1:822783/1‑140 (MQ=255)

                                                                                                             aaaGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAAAAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGa                       >  2:1632291/1‑140 (MQ=255)

                                                                                                                    aTTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCgg                >  1:868665/1‑140 (MQ=255)

                                                                                                                      tAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCa              <  1:1670459/140‑1 (MQ=255)

                                                                                                                      tAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCa              <  2:1328106/140‑1 (MQ=255)

                                                                                                                        gCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGa            <  1:409492/140‑1 (MQ=255)

                                                                                                                         ccAGAGGGAATCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc           <  1:1174330/140‑1 (MQ=255)

                                                                                                                         ccAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc           <  1:1625009/140‑1 (MQ=255)

                                                                                                                         ccAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc           <  1:1574734/140‑1 (MQ=255)

                                                                                                                         ccAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc           <  2:362729/140‑1 (MQ=255)

                                                                                                                             agGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTa       <  1:392084/140‑1 (MQ=255)

                                                                                                                              gggAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc      >  1:547508/1‑140 (MQ=255)

                                                                                                                              gggAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc      <  2:106166/140‑1 (MQ=255)

                                                                                                                              gggAAGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc      >  1:106166/1‑140 (MQ=255)

                                                                                                                                 aaGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc      <  1:370540/137‑1 (MQ=255)

                                                                                                                                 aaGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc      >  2:370540/1‑137 (MQ=255)

                                                                                                                                  aGCTCACGCC‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGt  >  1:335042/1‑140 (MQ=255)

ACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑C‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGT  >  NC_000913/2824335‑2824603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A