Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,827,717	A→G	E119G (GAA→GGA)	srlB →	sorbitol‑specific PTS enzyme IIA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,827,717	0	A	G	100.0%	186.3 / NA	53	E119G (GAA→GGA)	srlB	sorbitol‑specific PTS enzyme IIA component
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/24); total (29/24)

GGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCGGTTGTCGATATTCAGAGCGACAA  >  NC_000913/2827580‑2827849

ggCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGAt                                                                                                                                    >  2:95930/1‑140 (MQ=255)

ggCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGAt                                                                                                                                    >  2:1148039/1‑140 (MQ=255)

     aaGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGtt                                                                                                                               <  1:1035526/140‑1 (MQ=255)

       gACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTaa                                                                                                                             <  2:383382/140‑1 (MQ=255)

           aCCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAg                                                                                                                         >  2:1188420/1‑140 (MQ=255)

           aCCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAg                                                                                                                         >  1:804653/1‑140 (MQ=255)

            ccTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGt                                                                                                                        >  2:729650/1‑140 (MQ=255)

                 gcgAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTaaaaa                                                                                                                   <  1:1188420/140‑1 (MQ=255)

                 gcgAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTaaaaa                                                                                                                   <  1:432511/140‑1 (MQ=255)

                 gcgAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGCTTGGATCTGTTAAGGAGTaaaaa                                                                                                                   >  2:553754/1‑140 (MQ=255)

                     aCTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGa                                                                                                               <  1:231075/140‑1 (MQ=255)

                      cTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGaa                                                                                                              <  1:397254/140‑1 (MQ=255)

                              tGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTg                                                                                                      <  1:1094286/140‑1 (MQ=255)

                              tGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTg                                                                                                      <  1:327169/140‑1 (MQ=255)

                                   cccTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGtt                                                                                                 >  1:1001304/1‑140 (MQ=255)

                                   cccTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGtt                                                                                                 <  1:729650/140‑1 (MQ=255)

                                       gcgcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCa                                                                                             >  1:41800/1‑140 (MQ=255)

                                        cgcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGGGTAAAAAATGAATCAGGTTGCCGTTGTCAt                                                                                            >  2:688584/1‑140 (MQ=255)

                                        cgcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCAt                                                                                            >  1:597471/1‑140 (MQ=255)

                                        cgcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCAt                                                                                            >  1:6701/1‑140 (MQ=255)

                                         gcTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATc                                                                                           >  1:1206416/1‑140 (MQ=255)

                                             cGATGGTTTAAACGAAGGGGAATTTCCGGGCATTTACCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCggtg                                                                                       <  2:1143485/140‑1 (MQ=255)

                                                  gTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTggg                                                                                  >  1:354281/1‑140 (MQ=255)

                                                             gCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAgg                                                                       >  2:627882/1‑140 (MQ=255)

                                                                  aTTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGGGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGt                                                                  <  1:688584/140‑1 (MQ=255)

                                                                           cACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGcc                                                         <  2:921033/140‑1 (MQ=255)

                                                                             cTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCAc                                                       <  2:597471/140‑1 (MQ=255)

                                                                                tCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGt                                                    <  1:1148039/140‑1 (MQ=255)

                                                                                tCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGt                                                    >  1:194137/1‑140 (MQ=255)

                                                                                tCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGt                                                    >  2:115120/1‑140 (MQ=255)

                                                                                       ggCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTg                                             <  2:505027/140‑1 (MQ=255)

                                                                                          aGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTgg                                                <  1:251466/134‑1 (MQ=255)

                                                                                          aGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTgg                                                >  2:251466/1‑134 (MQ=255)

                                                                                          aGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCg                                          >  2:634669/1‑140 (MQ=255)

                                                                                          aGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCg                                          >  2:1052888/1‑140 (MQ=255)

                                                                                          aGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCg                                          >  2:1132239/1‑140 (MQ=255)

                                                                                            gCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAg                                        >  1:520845/1‑140 (MQ=255)

                                                                                            gCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAg                                        >  2:229652/1‑140 (MQ=255)

                                                                                            gCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAg                                        <  1:553754/140‑1 (MQ=255)

                                                                                              ccTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAggg                                      >  2:1047197/1‑140 (MQ=255)

                                                                                                 gTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGc                                            <  2:49236/131‑1 (MQ=255)

                                                                                                 gTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGc                                            >  1:49236/1‑131 (MQ=255)

                                                                                                  tCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCg                                          <  2:173739/132‑1 (MQ=255)

                                                                                                  tCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCg                                          >  1:173739/1‑132 (MQ=255)

                                                                                                  tCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTAt                                  >  2:536613/1‑140 (MQ=255)

                                                                                                         cgaTATCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTAtcgcgtcg                           <  2:520845/140‑1 (MQ=255)

                                                                                                           atatCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTAtcgcgtcgcg                         <  2:354281/140‑1 (MQ=255)

                                                                                                            tatCGCGCCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCgg                        >  1:941756/1‑140 (MQ=255)

                                                                                                                  gcCGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCGGTTGTCg                  <  1:634669/140‑1 (MQ=255)

                                                                                                                   ccGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCgg                        <  1:719818/133‑1 (MQ=255)

                                                                                                                   ccGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCgg                        >  2:719818/1‑133 (MQ=255)

                                                                                                                   ccGGGATCGGTTTTGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCGGTTGTCGa                 >  1:904954/1‑140 (MQ=255)

                                                                                                                                tGAAGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCGGTTGTCGATATTCAGAGCGAc    <  2:1089304/140‑1 (MQ=255)

                                                                                                                                  aaGTTTGGATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCGGTTGTCGATATTCAGAGCGACaa  <  1:115120/140‑1 (MQ=255)

GGCGGAAGACAACCTTCGCGAACTGGGTCATGTCACCCTGCGCTTCGATGGTTTAAACGAAGCGGAATTTCCGGGCACTGTCCATGTGGCAGGCCCTGTCCCCGACGATATCGCGCCGGGATCGGTTTTGAAGTTTGAATCTGTTAAGGAGTAAAAAATGAATCAGGTTGCCGTTGTCATCGGTGGTGGGCAAACCTTAGGCGCGTTCCTGTGCCACGGTCTGGCTGCCGAGGGGTATCGCGTCGCGGTTGTCGATATTCAGAGCGACAA  >  NC_000913/2827580‑2827849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A