Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,832,059 T→C E77E (GAA→GAG)  norR ← DNA‑binding transcriptional dual regulator NorR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,832,0590TC100.0% 199.7 / NA 56E77E (GAA→GAG) norRDNA‑binding transcriptional dual regulator NorR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/28);  total (28/28)

TGCCCGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTA  >  NC_000913/2831925‑2832195
                                                                                                                                      |                                                                                                                                        
tGCCCGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACCCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAg                                                                                                                                     <  2:229947/140‑1 (MQ=255)
      gCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTgg                                                                                                                               >  2:247174/1‑140 (MQ=255)
                    aCCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAgcg                                                                                                                 >  2:755924/1‑140 (MQ=255)
                    aCCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAgcg                                                                                                                 >  2:657339/1‑140 (MQ=255)
                     ccAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAgcgc                                                                                                                <  2:586596/140‑1 (MQ=255)
                      cAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCa                                                                                                               <  2:267475/140‑1 (MQ=255)
                         cGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAg                                                                                                            >  2:313603/1‑140 (MQ=255)
                          gCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGc                                                                                                           <  2:159360/140‑1 (MQ=255)
                            aGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGt                                                                                                         <  2:1109513/140‑1 (MQ=255)
                              gCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCt                                                                                                       >  2:372055/1‑140 (MQ=255)
                               cGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTa                                                                                                      <  2:72934/140‑1 (MQ=255)
                                        tCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACa                                                                                             >  1:193639/1‑140 (MQ=255)
                                        tCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACa                                                                                             >  1:993007/1‑140 (MQ=255)
                                          aGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATc                                                                                           <  1:755924/140‑1 (MQ=255)
                                          aGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATc                                                                                           <  1:930908/140‑1 (MQ=255)
                                                tcCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGc                                                                                     >  1:117961/1‑140 (MQ=255)
                                                          gAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATc                                                                                           <  1:217981/124‑1 (MQ=255)
                                                          gAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATc                                                                                           >  2:217981/1‑124 (MQ=255)
                                                          gAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCg                                                                           >  2:635502/1‑140 (MQ=255)
                                                          gAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCg                                                                           >  2:798934/1‑140 (MQ=255)
                                                            aTCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGAt                                                                         >  1:1111127/1‑140 (MQ=255)
                                                               aaaCCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGc                                                                      <  1:635502/140‑1 (MQ=255)
                                                               aaaCCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGc                                                                      >  2:156598/1‑140 (MQ=255)
                                                                 aCCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCaa                                                                    <  1:372055/140‑1 (MQ=255)
                                                                  ccGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAg                                                                   <  1:247174/140‑1 (MQ=255)
                                                                    gTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCg                                                                 >  2:911363/1‑140 (MQ=255)
                                                                             aTCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACt                                                        >  2:460736/1‑140 (MQ=255)
                                                                               cGGGCAATTCGCTGTCTGCGGGAGAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                      >  1:242041/1‑140 (MQ=255)
                                                                               cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAg                                                                   >  1:973682/1‑127 (MQ=255)
                                                                               cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAg                                                                   >  2:18893/1‑127 (MQ=255)
                                                                               cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAg                                                                   <  2:973682/127‑1 (MQ=255)
                                                                               cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAg                                                                   <  1:18893/127‑1 (MQ=255)
                                                                               cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                      >  2:842874/1‑140 (MQ=255)
                                                                               cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                      >  2:954356/1‑140 (MQ=255)
                                                                               cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                      >  2:356960/1‑140 (MQ=255)
                                                                               cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                      >  2:994600/1‑140 (MQ=255)
                                                                               cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                      >  2:225726/1‑140 (MQ=255)
                                                                                 ggCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCcg                                                    >  2:1126882/1‑140 (MQ=255)
                                                                                   cAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCcgcg                                                  <  1:798934/140‑1 (MQ=255)
                                                                                        cGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCg                                             <  1:1126882/140‑1 (MQ=255)
                                                                                           tGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCGAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATAGTaa                                          >  2:1041069/1‑140 (MQ=255)
                                                                                                gCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAg                                     <  1:225726/140‑1 (MQ=255)
                                                                                                       aGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGt                                            >  2:374791/1‑126 (MQ=255)
                                                                                                       aGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGt                                            <  1:374791/126‑1 (MQ=255)
                                                                                                       aGCGCACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCa                              >  2:288081/1‑140 (MQ=255)
                                                                                                         cgcACCACATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGa                            >  2:259824/1‑140 (MQ=255)
                                                                                                           caccacATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACg                          <  2:516476/140‑1 (MQ=255)
                                                                                                           caccacATCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACg                          <  2:327457/140‑1 (MQ=255)
                                                                                                                caTCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCg                     <  2:1111127/140‑1 (MQ=255)
                                                                                                                caTCCCCGGCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCg                     <  1:460736/140‑1 (MQ=255)
                                                                                                                       ggCGCGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCa              <  2:993007/140‑1 (MQ=255)
                                                                                                                        gcgcGGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAg             <  2:193639/140‑1 (MQ=255)
                                                                                                                           cgGGCAATCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGACGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCAc          <  2:847009/140‑1 (MQ=255)
                                                                                                                                 aTCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACg    <  2:242041/140‑1 (MQ=255)
                                                                                                                                 aTCGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACg    <  1:356960/140‑1 (MQ=255)
                                                                                                                                   cGCCTCCAGCCGTGGATGCCTTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCTCTCCAGCACCTGACGTa  <  2:94434/140‑1 (MQ=255)
                                                                                                                                   cGCCTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTa  <  1:657339/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
TGCCCGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTA  >  NC_000913/2831925‑2832195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A