Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,353	T→C	G304G (GGA→GGG)	ascG ←	DNA‑binding transcriptional repressor AscG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,353	0	T	C	100.0%	110.6 / NA	34	G304G (GGA→GGG)	ascG	DNA‑binding transcriptional repressor AscG
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/21); total (13/21)

CATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGC  >  NC_000913/2838217‑2838482

cATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCa                                                                                                                                <  2:585153/140‑1 (MQ=255)

       acTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAAttt                                                                                                                         <  2:622745/140‑1 (MQ=255)

       acTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAAttt                                                                                                                         <  1:518186/140‑1 (MQ=255)

        cTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGATGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTc                                                                                                                        <  2:110409/140‑1 (MQ=255)

         tAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTcc                                                                                                                       >  2:314754/1‑140 (MQ=255)

           gTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTg                                                                                                                     >  1:754624/1‑140 (MQ=255)

             gACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGaa                                                                                                                   <  2:431488/140‑1 (MQ=255)

             gACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGaa                                                                                                                   <  1:449180/140‑1 (MQ=255)

                   gACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCAtct                                                                                                             >  1:538170/1‑140 (MQ=255)

                     cATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGGAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAGTAATTTCCTGAATCATCTCa                                                                                                           >  2:279525/1‑140 (MQ=255)

                      aTGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAg                                                                                                          >  1:285267/1‑140 (MQ=255)

                           atgaCAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCAcc                                                                                                     >  2:487029/1‑140 (MQ=255)

                              aCAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGa                                                                                                  >  1:836668/1‑140 (MQ=255)

                                  tGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAAttt                                                                                              >  1:491343/1‑140 (MQ=255)

                                     ttATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGGAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAGTAATTTCCTGAATCATCTCAGTCACCGGAATTTTTa                                                                                           <  1:279525/140‑1 (MQ=255)

                                        tCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGc                                                                                        >  2:273799/1‑140 (MQ=255)

                                            gAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGga                                                                                    >  1:726861/1‑140 (MQ=255)

                                              aGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGgaga                                                                                  >  1:369546/1‑140 (MQ=255)

                                              aGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAAACAGCCGCCCAAAAATTTCCTTAATCATCCCAATCACCGGAATTTTTAAGCTGgaga                                                                                  >  2:839414/1‑140 (MQ=255)

                                                     aTGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCgg                                                                           <  2:357067/140‑1 (MQ=255)

                                                      tgagtgagTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCAGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGa                                                                          <  1:737362/140‑1 (MQ=255)

                                                       gagtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGaa                                                                         <  2:87137/140‑1 (MQ=255)

                                                        agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAAc                                                                        <  2:289165/140‑1 (MQ=255)

                                                            agtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGgtg                                                                    <  2:754624/140‑1 (MQ=255)

                                                                    cggATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGc                                                            <  2:836668/140‑1 (MQ=255)

                                                                       aTCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAAt                                                         <  2:369546/140‑1 (MQ=255)

                                                                         cAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAg                                                       <  1:487029/140‑1 (MQ=255)

                                                                                    tGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCg                                            <  1:364510/140‑1 (MQ=255)

                                                                                      aaGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGaa                                          <  1:380414/140‑1 (MQ=255)

                                                                                             tCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATa                                   >  2:252934/1‑140 (MQ=255)

                                                                                                      agaAATCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACAcc                          >  1:291766/1‑140 (MQ=255)

                                                                                                         aaTCCCCGCCATCGAGCATAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGc                       <  1:839414/140‑1 (MQ=255)

                                                                                                                           tAAAAATCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTAcgccg     <  1:273799/140‑1 (MQ=255)

                                                                                                                             aaaaTCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCcgcg   <  2:285267/140‑1 (MQ=255)

                                                                                                                              aaaTCAGCCGCCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCgcgc  <  2:380829/140‑1 (MQ=255)

CATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGC  >  NC_000913/2838217‑2838482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A