Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,031,084	T→C	intergenic (‑153/‑283)	ygfT ← / → uacT	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit/urate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,031,084	0	T	C	100.0%	212.3 / NA	60	intergenic (‑153/‑283)	ygfT/uacT	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit/urate:H(+) symporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/26); total (34/26)

TTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACATTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCG  >  NC_000913/3030955‑3031215

ttATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTg                                                                                                                                   >  2:619042/1‑132 (MQ=255)

ttATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTg                                                                                                                                   <  1:619042/132‑1 (MQ=255)

ttATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCaa                                                                                                                           >  2:603711/1‑140 (MQ=255)

ttATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCaa                                                                                                                           >  2:448695/1‑140 (MQ=255)

     tcttctCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAagag                                                                                                                      <  2:783293/140‑1 (MQ=255)

       ttctCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAgcg                                                                                                                    <  2:709840/140‑1 (MQ=255)

       ttctCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAgcg                                                                                                                    <  2:467138/140‑1 (MQ=255)

       ttctCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAgcg                                                                                                                    <  1:72075/140‑1 (MQ=255)

          tcAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTa                                                                                                                 >  2:603558/1‑140 (MQ=255)

                         cAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGa                                                                                                  <  1:167456/140‑1 (MQ=255)

                                cGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATg                                                                                           <  1:160070/140‑1 (MQ=255)

                                   gCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGc                                                                                          >  2:563499/1‑138 (MQ=255)

                                   gCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGc                                                                                          <  1:563499/138‑1 (MQ=255)

                                   gCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCga                                                                                        >  1:768757/1‑140 (MQ=255)

                                    cATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCgag                                                                                       >  1:339661/1‑140 (MQ=255)

                                        tatTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTg                                                                                   >  1:500011/1‑140 (MQ=255)

                                           tAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGa                                                                                <  2:227790/140‑1 (MQ=255)

                                           tAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGa                                                                                <  1:603558/140‑1 (MQ=255)

                                                aGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGc                                                                           >  1:754415/1‑140 (MQ=255)

                                                 gCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCGGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGcc                                                                          >  2:797378/1‑140 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAATTTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCa                                                                         >  2:722611/1‑140 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGa                                                                                >  1:304444/1‑133 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGa                                                                                <  2:304444/133‑1 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATg                                                                            >  1:691895/1‑137 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATg                                                                            <  2:691895/137‑1 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATg                                                                            <  1:738222/137‑1 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATg                                                                            >  2:738222/1‑137 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCa                                                                         >  1:131460/1‑140 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCa                                                                         >  1:241105/1‑140 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCa                                                                         >  1:279298/1‑140 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCa                                                                         >  1:842600/1‑140 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCa                                                                         >  2:533046/1‑140 (MQ=255)

                                                  cATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTAAGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCa                                                                         >  2:732263/1‑140 (MQ=255)

                                                   aTGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCg                                                                                         <  2:443049/123‑1 (MQ=255)

                                                   aTGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCg                                                                                         >  1:443049/1‑123 (MQ=255)

                                                    tGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGa                                                                       <  2:754415/140‑1 (MQ=255)

                                                    tGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGa                                                                       <  2:247054/140‑1 (MQ=255)

                                                    tGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGa                                                                       <  2:500011/140‑1 (MQ=255)

                                                       ccGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATg                                                                    >  2:368961/1‑140 (MQ=255)

                                                       ccGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATg                                                                    >  1:275622/1‑140 (MQ=255)

                                                              aaTTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATg                                                             <  1:368961/140‑1 (MQ=255)

                                                                         tGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAata                                                  >  1:190125/1‑140 (MQ=255)

                                                                          gCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAatat                                                 >  2:601318/1‑140 (MQ=255)

                                                                                      aaaTTATTGCCCCTCAATCTAAGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACa                                     <  1:732263/140‑1 (MQ=255)

                                                                                        attattGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGTGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGa                                   <  1:448695/140‑1 (MQ=255)

                                                                                             tGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATa                                 >  2:615476/1‑137 (MQ=255)

                                                                                             tGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATa                                 <  1:615476/137‑1 (MQ=255)

                                                                                             tGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAAtt                              <  2:339661/140‑1 (MQ=255)

                                                                                             tGCCCCTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAAtt                              <  1:603711/140‑1 (MQ=255)

                                                                                                cccTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCa                           <  1:601318/140‑1 (MQ=255)

                                                                                                  cTCAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAATGCGACATGACAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCAtt                         >  2:798276/1‑140 (MQ=255)

                                                                                                    cAATCTATGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGt                       >  1:454069/1‑140 (MQ=255)

                                                                                                           tGCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGTCAGGttc                >  2:728022/1‑140 (MQ=255)

                                                                                                            gCCGCTTTCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGTCAGGttct               >  2:869172/1‑140 (MQ=255)

                                                                                                                 tttCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGTCAGGttctcttct          >  2:5611/1‑140 (MQ=255)

                                                                                                                 tttCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGTCAGGttctcttct          >  2:458533/1‑140 (MQ=255)

                                                                                                                 tttCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGTCAGGttctcttct          <  2:190125/140‑1 (MQ=255)

                                                                                                                 tttCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGTCAGGttctcttct          >  1:482156/1‑140 (MQ=255)

                                                                                                                  ttCATTATTTCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGTCAGGttctcttctc         >  1:181345/1‑140 (MQ=255)

                                                                                                                         tttCAACACTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCg  <  1:533046/140‑1 (MQ=255)

TTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACATTGCCGATCAAAAGAGCGCTATCCAGATAAAAACGATTAAATGCGAGAGTGCGACATGCCAGAATGATTAATGAAATATCAATATGAGAAAATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCG  >  NC_000913/3030955‑3031215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A