Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,107,824	G→A	G444G (GGC→GGT)	speC ←	ornithine decarboxylase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,107,824	0	G	A	100.0%	124.4 / NA	38	G444G (GGC→GGT)	speC	ornithine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/22); total (16/22)

AGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGG  >  NC_000913/3107687‑3107954

aGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTAcc                                                                                                                                  >  1:390928/1‑140 (MQ=255)

  tAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCAt                                                                                                                                >  2:371460/1‑140 (MQ=255)

   aaCAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATc                                                                                                                               <  1:371460/140‑1 (MQ=255)

   aaCAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCCCACACTTATGTCGGGTAATCCTGCCCCAATTTAACATc                                                                                                                               >  2:135921/1‑140 (MQ=255)

          tGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAAcg                                                                                                                        >  1:533385/1‑140 (MQ=255)

                gATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAAcgggcggg                                                                                                                  >  1:503904/1‑140 (MQ=255)

                  tCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGAt                                                                                                                >  2:418067/1‑140 (MQ=255)

                    aacaaAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATaa                                                                                                              <  2:250194/140‑1 (MQ=255)

                           tACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCg                                                                                                       >  1:646267/1‑140 (MQ=255)

                             cTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGa                                                                                                     <  1:175467/140‑1 (MQ=255)

                             cTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGa                                                                                                     <  2:263162/140‑1 (MQ=255)

                             cTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGa                                                                                                     <  2:126597/140‑1 (MQ=255)

                             cTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGa                                                                                                     <  1:22349/140‑1 (MQ=255)

                                   cgcgGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCt                                                                                               <  2:503904/140‑1 (MQ=255)

                                     cgGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGa                                                                                                     <  1:522516/132‑1 (MQ=255)

                                     cgGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGa                                                                                                     >  2:522516/1‑132 (MQ=255)

                                        cATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAg                                                                                          <  1:76797/140‑1 (MQ=255)

                                            tCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAgcgcg                                                                                      <  1:729410/140‑1 (MQ=255)

                                            tCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAgcgcg                                                                                      <  1:54393/140‑1 (MQ=255)

                                                 cAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACCGATGTCCGATAAATCCGCCACCATTCACCACCAACAACGGGCCGGATAAACCGGCGGAAGAGCCTACAGCCCGCAAGa                                                                                 >  2:524377/1‑140 (MQ=255)

                                                      ccGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGc                                                                            >  1:35397/1‑140 (MQ=255)

                                                       cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGcc                                                                           >  1:715025/1‑140 (MQ=255)

                                                       cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGcc                                                                           >  2:144841/1‑140 (MQ=255)

                                                         tGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGaa                                                                                <  1:792917/133‑1 (MQ=255)

                                                         tGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGaa                                                                                >  2:792917/1‑133 (MQ=255)

                                                                   cccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCa                                                               <  1:21645/140‑1 (MQ=255)

                                                                        ggCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATccc                                                          <  2:139947/140‑1 (MQ=255)

                                                                           tCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAt                                                       <  1:144841/140‑1 (MQ=255)

                                                                                 cTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAac                                                 <  1:243431/140‑1 (MQ=255)

                                                                                      aaaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACt                                            <  2:646267/140‑1 (MQ=255)

                                                                                                             cTGATGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCActc                     <  2:35397/140‑1 (MQ=255)

                                                                                                                aTGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcc                  >  2:229838/1‑140 (MQ=255)

                                                                                                                aTGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcc                  >  1:377327/1‑140 (MQ=255)

                                                                                                                aTGTCGGATAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcc                  >  1:758005/1‑140 (MQ=255)

                                                                                                                       aTAATCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCAt           >  2:697410/1‑140 (MQ=255)

                                                                                                                          aTCCTGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGaa        >  2:96393/1‑140 (MQ=255)

                                                                                                                              tGCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAAtttt    <  2:377327/140‑1 (MQ=255)

                                                                                                                               gCCACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTg   <  1:697410/140‑1 (MQ=255)

                                                                                                                                ccACAATTTACCATCAACAACGGGCGGGGTAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTCGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTgg  <  2:226908/140‑1 (MQ=255)

                                                                                                                                ccACAATTTACCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTgg  <  2:390928/140‑1 (MQ=255)

AGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGG  >  NC_000913/3107687‑3107954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A