Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 163 52.9 1786516 99.3% 1774010 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,120,523 T→C T253A (ACT→GCT)  glcA ← glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,120,5230TC95.2% 203.9 / 0.3 63T253A (ACT→GCT) glcAglycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (30/30);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

ACGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGTAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAAC  >  NC_000913/3120387‑3120659
                                                                                                                                        |                                                                                                                                        
aCGGGACCGCCAGAAGATGTCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAat                                                                                                                                       >  2:61852/1‑140 (MQ=255)
aCGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAat                                                                                                                                       >  1:602461/1‑140 (MQ=255)
 cGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAata                                                                                                                                      <  2:214777/140‑1 (MQ=255)
 cGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAata                                                                                                                                      <  2:232663/140‑1 (MQ=255)
         ccAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAg                                                                                                                              >  1:343312/1‑140 (MQ=255)
            gaagaTGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTc                                                                                                                           <  1:39165/140‑1 (MQ=255)
                aTGTCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGa                                                                                                                       <  1:61852/140‑1 (MQ=255)
                 tGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGAc                                                                                                                      <  1:463341/140‑1 (MQ=255)
                  ggCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGAcc                                                                                                                     <  1:563324/140‑1 (MQ=255)
                   gCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCa                                                                                                                    >  1:171772/1‑140 (MQ=255)
                   gCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCa                                                                                                                    >  1:132591/1‑140 (MQ=255)
                    cTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCaa                                                                                                                   <  1:341186/140‑1 (MQ=255)
                    cTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCaa                                                                                                                   >  1:749956/1‑140 (MQ=255)
                    cTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCaa                                                                                                                   <  1:283402/140‑1 (MQ=255)
                       aTTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCgg                                                                                                                                 >  2:326148/1‑123 (MQ=255)
                       aTTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCgg                                                                                                                                 <  1:326148/123‑1 (MQ=255)
                             ctccaTCGCACCTGCGGATTGTGCCATGCTGATTGCTGTTGCGGTTTTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCGCCAGCGCCGAAGTAATATCCGGCAGTTCCGGACCAATATAGTTag                                                                                                          <  2:376102/138‑1 (MQ=255)
                             caccaTCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTag                                                                                                          <  2:890073/140‑1 (MQ=255)
                                  tCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAata                                                                                                                 <  1:445547/128‑1 (MQ=255)
                                  tCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAata                                                                                                                 >  2:445547/1‑128 (MQ=255)
                                  tCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTa                                                                                                     >  1:597507/1‑140 (MQ=255)
                                  tCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTa                                                                                                     >  1:689144/1‑140 (MQ=255)
                                  tCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTa                                                                                                     >  2:772426/1‑140 (MQ=255)
                                       ccTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGaa                                                                                                >  1:64984/1‑140 (MQ=255)
                                          gCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTGAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGTAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTg                                                                                             >  2:546211/1‑140 (MQ=255)
                                          gCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTg                                                                                             >  2:839176/1‑140 (MQ=255)
                                                tGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGAc                                                                                       <  2:690890/140‑1 (MQ=255)
                                                  tCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAg                                                                                     >  1:61282/1‑140 (MQ=255)
                                                    ccATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCg                                                                                   <  2:749956/140‑1 (MQ=255)
                                                        gCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGc                                                                               >  1:450745/1‑140 (MQ=255)
                                                            aTTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTcc                                                                           >  2:402746/1‑140 (MQ=255)
                                                                 cGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCTATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAg                                                                      >  2:639426/1‑140 (MQ=255)
                                                                 cGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAACGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTc                                                                            <  1:882961/134‑1 (MQ=255)
                                                                 cGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAACGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTc                                                                            >  2:882961/1‑134 (MQ=255)
                                                                   tttCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCa                                                                    >  2:475396/1‑140 (MQ=255)
                                                                    ttCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCaa                                                                   >  2:611665/1‑140 (MQ=255)
                                                                        gTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAg                                                               <  2:64984/140‑1 (MQ=255)
                                                                           tttttCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAgcgc                                                            <  2:597507/140‑1 (MQ=255)
                                                                                cGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTg                                                       >  1:248058/1‑140 (MQ=255)
                                                                                 ggCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTgg                                                      <  1:611665/140‑1 (MQ=255)
                                                                                   ccgccAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGc                                                     >  2:858482/1‑139 (MQ=255)
                                                                                   ccgccAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGc                                                     <  1:858482/139‑1 (MQ=255)
                                                                                     gccAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCAc                                                  <  2:61282/140‑1 (MQ=255)
                                                                                             ttAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCttt                                          <  1:772426/140‑1 (MQ=255)
                                                                                                      aaaGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCAc                                       <  1:589180/134‑1 (MQ=255)
                                                                                                      aaaGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCAc                                       >  2:589180/1‑134 (MQ=255)
                                                                                                        aGCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTcc                               <  1:800666/140‑1 (MQ=255)
                                                                                                         gCGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCa                              <  2:343312/140‑1 (MQ=255)
                                                                                                          cGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAg                             <  1:775458/140‑1 (MQ=255)
                                                                                                          cGAGTGAGACGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAg                             <  2:248058/140‑1 (MQ=255)
                                                                                                                  aCGATACTCACCAGCGCCGAAGTAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCcat                     <  1:546211/140‑1 (MQ=255)
                                                                                                                  aCGATACTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCcat                     <  2:450745/140‑1 (MQ=255)
                                                                                                                       aCTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTg                >  2:558101/1‑140 (MQ=255)
                                                                                                                        cTCACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGc               >  1:573939/1‑140 (MQ=255)
                                                                                                                          cACCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTa             <  1:402746/140‑1 (MQ=255)
                                                                                                                           aCCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCca                      >  2:658909/1‑130 (MQ=255)
                                                                                                                           aCCAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCca                      <  1:658909/130‑1 (MQ=255)
                                                                                                                             cAGCGCCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTAcca          >  1:53900/1‑140 (MQ=255)
                                                                                                                               gcgcCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTAccagc        <  1:839176/140‑1 (MQ=255)
                                                                                                                                cgcCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTAccagcc       >  2:310731/1‑140 (MQ=255)
                                                                                                                                cgcCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTAccagcc       >  2:258229/1‑140 (MQ=255)
                                                                                                                                cgcCGAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTAccagcc       >  2:390940/1‑140 (MQ=255)
                                                                                                                                    gAAGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGaa   <  1:389186/140‑1 (MQ=255)
                                                                                                                                     aaGCAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAAc  >  1:53291/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
ACGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTGCCGTTTCGGTATTTTTCGGCCGCCAGACTTTAAGGAATAAAGCGAGTGAGACGATACTCACCAGCGCCGAAGTAATATCCGGCAGTTCCGGACCAATATAGTTAGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAAC  >  NC_000913/3120387‑3120659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A