Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,236,458	A→G	I167V (ATC→GTC)	ygjP →	putative metal‑dependent hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,236,458	0	A	G	97.5%	132.1 / ‑4.2	40	I167V (ATC→GTC)	ygjP	putative metal‑dependent hydrolase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (24/15); total (25/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

ACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGA  >  NC_000913/3236321‑3236591

aCGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTc                                                                                                                                     >  1:440747/1‑140 (MQ=255)

       ggCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAat                                                                                                                              <  2:444876/140‑1 (MQ=255)

            aCCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGt                                                                                                                         <  2:194439/140‑1 (MQ=255)

               tGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAg                                                                                                                      <  1:272854/140‑1 (MQ=255)

                        aaGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtct                                                                                                             >  2:46959/1‑140 (MQ=255)

                        aaGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtct                                                                                                             <  1:334243/140‑1 (MQ=255)

                          gagCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtcttt                                                                                                           <  2:257855/140‑1 (MQ=255)

                           agCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtctttt                                                                                                          >  1:151134/1‑140 (MQ=255)

                              aTAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTAtcttttctt                                                                                                       >  1:829792/1‑140 (MQ=255)

                                 aCAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTttctttct                                                                                                    >  1:536610/1‑140 (MQ=255)

                                     aGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAg                                                                                                >  2:121413/1‑140 (MQ=255)

                                       cGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAgcg                                                                                              >  1:746194/1‑140 (MQ=255)

                                       cGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAgcg                                                                                              >  1:447223/1‑140 (MQ=255)

                                               cAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAAt                                                                                      <  2:151134/140‑1 (MQ=255)

                                                  ttgtgttgtCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGa                                                                                   >  1:537271/1‑140 (MQ=255)

                                                          tCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTccccc                                                                           <  2:447223/140‑1 (MQ=255)

                                                          tCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTccccc                                                                           >  1:320427/1‑140 (MQ=255)

                                                            atatGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCt                                                                         >  2:355395/1‑140 (MQ=255)

                                                             tatGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCtt                                                                        <  1:121413/140‑1 (MQ=255)

                                                                 gAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGt                                                                    <  1:355395/140‑1 (MQ=255)

                                                                  aaCCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGtt                                                                   <  2:146158/140‑1 (MQ=255)

                                                                    ccccAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTaa                                                                 >  1:857497/1‑140 (MQ=255)

                                                                       cAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGa                                                              >  2:682936/1‑140 (MQ=255)

                                                                        aGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGAt                                                             <  2:746194/140‑1 (MQ=255)

                                                                                  gCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAtg                                                   >  1:736856/1‑140 (MQ=255)

                                                                                   cTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCAtgc                                                  >  1:366963/1‑140 (MQ=255)

                                                                                              cacCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCAttt                                       >  1:64153/1‑140 (MQ=255)

                                                                                                 ccGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGcc                                    >  2:784198/1‑140 (MQ=255)

                                                                                                       gtgGCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCctt                              <  1:46959/140‑1 (MQ=255)

                                                                                                          gCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttctt                           >  1:271676/1‑140 (MQ=255)

                                                                                                          gCTAACGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCcttctt                           <  2:440747/140‑1 (MQ=255)

                                                                                                               cGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCg                      >  1:252415/1‑140 (MQ=255)

                                                                                                               cGCAGTTGTCGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCg                      >  1:161151/1‑140 (MQ=255)

                                                                                                                        cGCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCAGCCTTCTTCTCCGTATTTGttt             >  2:702954/1‑140 (MQ=255)

                                                                                                                         gCTTGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGtttt            >  1:862192/1‑140 (MQ=255)

                                                                                                                            tGGGCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTtct         <  2:857497/140‑1 (MQ=255)

                                                                                                                              ggCAGAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCg       >  2:441637/1‑140 (MQ=255)

                                                                                                                                  gAACAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCgg   <  2:366963/140‑1 (MQ=255)

                                                                                                                                   aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGa  >  2:385508/1‑140 (MQ=255)

                                                                                                                                   aaCAAAGTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGa  >  2:856064/1‑140 (MQ=255)

ACGAGCTGGCGCACCTGAAGGAGAAAGAGCATAACAAAGCGTTTTACCAGTTGTGTTGTCATATGGAACCCCAGTACCACCAGCTTGAGTTCGACACCCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGA  >  NC_000913/3236321‑3236591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A